The Pea Gene LH Encodes ent-Kaurene Oxidase
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Abstract The pea (Pisum sativum) homolog, PsKO1, of the Arabidopsis GA3 gene was isolated. It codes for a cytochrome P450 from the CYP701A subfamily and has ent-kaurene oxidase (KO) activity, catalyzing the three step oxidation of ent-kaurene to ent-kaurenoic acid in the gibberellin (GA) biosynthetic pathway when expressed in yeast (Saccharomyces cerevisiae). PsKO1 is encoded by the LH gene because in three independent mutant alleles, lh-1, lh-2, and lh-3, PsKO1 has altered sequence, and the lh-1 allele, when expressed in yeast, failed to metabolize ent-kaurene. The lh mutants of pea are GA deficient and have reduced internode elongation and root growth. One mutant (lh-2) also causes a large increase in seed abortion. PsKO1 (LH) is expressed in all tissues examined, including stems, roots, and seeds, and appears to be a single-copy gene. Differences in sensitivity to the GA synthesis inhibitor, paclobutrazol, between the mutants appear to result from the distinct nature of the genetic lesions. These differences may also explain the tissue-specific differences between the mutants.Keywords:
Paclobutrazol
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A research investigation was conducted to find out how Nickel's different stresses affected the morphological alterations in Pisum sativum. Pisum sativum has a complex stem structure. In the transactional view, the stem of Pisum sativum is usually more or less square-shaped. The root's transverse section has a form which is slightly rounded. The structural abnormalities of Pisum sativum treated with different concentrations of Nickel indicated its toxic effect on the anatomical structure of the root and stem. Pisum sativum treated with lower to higher doses of Nickel showed few specific effects on stem anatomy. Plants treated with nickel had many structural changes in their roots.
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Abstract The relation between the action of gibberellin A 3 (GA 3 ) and the synthesis of DNA, RNA and proteins in plant cells, is known in several plant species (2,4,6). However, previous investigations have not shown the effect of GA 3 on each of these syntheses in tissue from the same plant. This communication shows the influence of GA 3 on each of these substances.
Plant cell
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In different growth period,we spray different concentraction mangic acid solution on the surface of leaves of Pisum Sativum L.. The result show that the activity of SOD, CAT is promoted,the content of MDA and the speed of O~(·-)_2 production are reduced in seedling, buding and flowering period.We find that if spraying 0.1%～0.2% mangic acid solution on the surface of leaves in seedling, buding and flowering period,the production of Pisum Sativum L.was promoted.
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Based on the citation of the literature,it was recounted that the research progress of Erysiphe pisi DC.of Pisum sativum L.at home and abroad,and the research status of Erysiphe pisi DC.of Pisum sativum L.at present in China.In addition,the Erysiphe pisi DC.of Pisum sativum L.has been prospected.
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In different growth periods,the different concentractions of manganese sulfate solutions are sprayed on the surface of leaves of Pisum Sativum.The results show that the activity of SOD,CAT is promoted,the content of MDA and the speed of O·-2 production are reduced in seeding,buding and flowering periods.Spraying 0.1%-0.2% of manganese sulfate solution on the surface of leaves of Pisum Sativum is best before flowering period.
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