The nucleotide sequence of RNA3 and RNA4 of olive latent virus 2
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Abstract:
Olive latent virus 2 (OLV2), a virus with particle shapes resembling those of alfalfa mosaic alfamovirus (AMV), has four major RNA species, two of which (RNA3 and RNA4) were completely sequenced. RNA3 was a bicistronic molecule containing two clear-cut ORFs, one of which (ORF1) coded for a 36.5 kDa polypeptide with conserved motifs of the '30K superfamily' movement proteins and the other (ORF2) encoded a 20 kDa polypeptide identified as the viral coat protein. RNA4, which was a little smaller than RNA3 (2078 nt versus 2438 nt), also differed from RNA3 in a few positions, but its in vitro translation did not produce any protein. By contrast, an additional RNA, 1042 nt in size with strong sequence homology with RNA3 and RNA4, was identified in RNA extracts from infected plants. This RNA, which may be a subgenomic form of RNA3 carrying the coat protein cistron, is apparently encapsidated to a very small extent, or not at all. Comparative computer-assisted analysis of virus-coded protein sequences disclosed homologies suggesting that OLV2 may belong to the family Bromoviridae, but as an entity separated from the currently known genera.Keywords:
Sequence (biology)
Latent Virus
Latent Virus
Mosaic virus
Alfalfa mosaic virus
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Latent Virus
Virus latency
Viral Shedding
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A latent influenza infection was produced experimentally in three ways: after experiencing the disease, after immunization with a live virus, as a result of vertical transmission of the virus persisting in females. In the latent influenza infection forming after the disease the duration of virus persistence was 112 days postinfection. The persisting virus from the animals receiving a single immunization was isolated only up to 35 days postinoculation. Both after the disease and immunization with a live virus the persisting infectious virus was found in the lungs in low titres not exceeding 1 lg EID50/0.2 ml. In contrast, a latent influenza infection in mice born to mothers-virus carriers was characterized by virus persistence in the blood and viscera in titres of 10(1) to 10(2) EID50/0.1 ml. Features of influenza virus persistence and conditions of its formation in mammals by the three ways mentioned are discussed.
Persistence (discontinuity)
Latent Virus
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There is potential for flight time based DNA sequencing involving disassembly into individual nucleotides which would pass through a nanochannel with two or more detectors. We performed molecular dynamics simulations of electrophoretic motion of single DNA nucleotides through 3 nm wide hydrophobic slits with both smooth and rough walls. The electric field (E) varied from 0.0 to 0.6 V/nm. The nucleotides adsorb and desorb from walls multiple times during their transit through the slit. The nucleotide–wall interactions differed due to nucleotide hydrophobicities and wall roughness which determined duration and frequency of nucleotide adsorptions and their velocities while adsorbed. Transient association of nucleotides with one, two, or three sodium ions occurred, but the mean association numbers (ANs) were weak functions of nucleotide type. Nucleotide–wall interactions contributed more to separation of nucleotide flight time distributions than ion association and thus indicate that nucleotide–wall interactions play a defining role in successfully discriminating between nucleotides on the basis of their flight times through nanochannels/slits. With smooth walls, smaller nucleotides moved faster, but with rough walls larger nucleotides moved faster due to fewer favorable wall adsorption sites. This indicates that roughness, or surface patterning, might be exploited to achieve better time-of-flight based discrimination between nucleotides.
Dynamics
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Bromodeoxyuridine
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Lymphocytic choriomeningitis virus has been used to set up a model of a latent virus infection in mice. It has been shown to be possible to induce reproducible virus infections in mice which remain completely symptom-free in spite of levels of viral growth equal to those found in the sick animal, by the inoculation of mice within a few hours of birth. This is a convenient method of producing a latent infection in the mice. The effect of the age of the mice at the time of intracerebral inoculation was studied with respect to the pattern of disease produced. Several methods were tried without success in order to induce overt disease in the latently infected animals. The virus did not cause any demonstrable cytopathogenic effect on mouse tissue and several other types of animal tissue. A slight cytopathogenic effect was observed in a strain of human cells in vitro. Virus persisted for weeks in some of the tissue cultures, without damaging the tissue but with the production of active virus. The bearing of the results obtained is discussed in relationship to current concepts of latent virus infection and particularly immunological tolerance. A concept of a special variety of latency is introduced and the name "vital" infection suggested for this.
Latent Virus
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Perchloric acid
Ribose
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We have successfully ligated the 12 nucleotide fragment (nucleotides 46-57) with the p19p nucleotide fragment (nucleotides 58-76) to form a 31p nucleotide fragment (nucleotides 46-76) which after 5'-phosphorylation was in turn joined to the 10 nucleotide fragment (nucleotides 36-45) to give the 3'-half molecule of yeast alanine tRNA.
Alanine
Fragment (logic)
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In this chapter we describe methods for the chemical synthesis of modified nucleosides and nucleotides. Because most naturally nucleosides and nucleotides are commercially available, syntheses have been directed at the generation of nucleosides containing modified bases and sugars and nucleotides in which oxygen atoms in the phosphate ester are replaced by other heteroatoms. The enzymatic processes associated with nucleotide metabolism are described and the roles of nucleotides in biology are discussed. Finally, the biological properties of nucleosides as antiviral and anticancer agents are highlighted.
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