Visualizing the Quadruplex: From Fluorescent Ligands to Light-Up Probes
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G-quadruplex
The effects of four different temperature such as T1(18℃ day/12℃ night),T2(24℃ day/18℃ night),T3(30℃ day/24℃ night),T4(36℃ day/30℃ night) treatments on soluble protein and nucleic acid were studied in Chinese Kale.The results showed that the content of soluble protein was the highest in 36℃ day/30℃ night treatment and the lowest in 30℃ day/24℃ night treatment.The contents of total nucleic acid,DNA and RNA in the stem apex decreased with prolongation of temperature.with prolongation of temperature treatment was prolonged,the contents of total nucleic acid and RNA in all treatments rose gradually after decreasing except for 36℃ day/30℃ night treatment which decreased continuously.The content of DNA showed a changing trend such as decreasing-increasing-decreasing except for 36℃ day/30℃ treatment which increased after decreasing.Low temperature could accelerated the synthesis of nucleic acid,maintained the stability of the nucleic acid,but high temperature inhibited the synthesis of the nucleic acid,DNA and RNA,and subsequently led to the decreasing of nucleic acid contents.
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This chapter contains sections titled: Introduction Detection of nucleic acid targets by nucleic acid probes Functional nucleic acids Fluorescent nucleic acid sensors Colorimetric nucleic acid sensors Electrochemical nucleic acid sensors Piezoelectric nucleic acid sensors Conclusion and perspectives References
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Molecular beacon
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G-quadruplex
Moiety
A-DNA
Sequence (biology)
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Nucleic acid structure
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Abstract The noncovalent interactions of night blue (NB) with several nucleic acids in buffer medium of Britton‐Robinson at pH 4.1 have been studied by spectroscopic methods. It is shown that the binding of NB with nucleic acids involves the J ‐aggregation of NB molecules on the surface of nucleic acids. The aggregation was encouraged by polyanions nucleic acids, in which nucleic acids served for acting templates. In this connection, a new method of nucleic acids with sensitivity at nanogram level is proposed based on the measurement of enhanced resonance light scattering (RLS). The linear range of ctDNA, fsDNA and yRNA is 0.01—2.5, 0.03—2.5 and 0.04—1.0 μg/mL, respectively, and the corresponding detection limits (3s̀) are 9.4, 7.3 and 5.7 ng/mL at 2.5 × 10– 5 mol/L of NB. Synthetic and real samples were analyzed with satisfactory results.
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CONTENTS I. Introduction 144 II. Structure and properties of nucleic acids 144 III. Effect of metal ions on the properties of nucleic acids 146 IV. Compounds of nucleic acids with metals 147 V. Structure of complexes of metals with nucleic acids 148 VI. Stability of complexes of metals with DNA 150
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Nucleic acid methods
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The noncovalent interactions of night blue (NB) with several nucleic acids in buffer medium of Britton-Robinson at pH 4.1 have been studied by spectroscopic methods.It is shown that the binding of NB with nucleic acids involves the J-aggregation of NB molecules on the surface of nucleic acids.The aggregation was encouraged by polyanions nucleic acids,in which nucleic acids served for acting templates,In this connection,a new method of nucleic acids with sensitivity at nanogram level is proposed based on the measurement of enhanced resonance light scattering (RLS).The linear range of ctDNA,fsDNA and yRNA is 0.01-2.5,0.03-2.5 and 0.04-1.0 μg/mL,respectively,and the corresponding detection limits(3σ）are 9.4,7.3 and 5.7ng/mL at 2.5×10^05mol/L of NB.Synthetic and real samples were analyzed with satisfactory results.
Nucleic acid structure
Nucleic acid quantitation
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