Molecular beacon–quantum dot–Au nanoparticle hybrid nanoprobes for visualizing virus replication in living cells
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Here we describe a new hybrid fluorescent nanoprobe composed of a nuclease-resistant molecular beacon (MB) backbone, CdSe-ZnS core-shell quantum dots (QDs) as donors, and gold nanoparticles (Au NPs) as quenchers, for the real-time visualization of virus replication in living cells. By using a Au NP-MB to QD ratio of 6 : 1, a 7.3-fold increase in fluorescent signal was achieved upon target binding. For living cell experiments, a hexahistidine-appended Tat peptide was self-assembled onto the QD surface to provide nearly 100% non-invasive delivery of the QD-MB-Au NP probes within 2 h. By directly visualizing the fluorescent complexes formed with the newly synthesized viral RNA, this QD-MB-Au NP probe provided sensitive and real-time detection of infectious viruses as well as the real-time visualization of cell-to-cell virus spreading.Keywords:
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In this study, a novel ratiometric fluorescent nanoprobe for pH monitoring has been developed by synthesizing red fluorescent Ag 2 S quantum dots (Ag 2 S QDs) and green fluorescent carbon dots (CDs) nanohybrids (Ag 2 S CDs) in one pot using CDs as templates. The nanoprobe exhibits dual-emission peaks at 500 and 670 nm under a single-excitation wavelength of 450 nm. The red fluorescence can be selectively quenched by increasing pH, while the green fluorescence is an internal reference. Therefore, the change of the relative fluorescence intensity (I 500 /I 670 ) in the ratiometric Ag 2 S CDs probes can be used for pH sensing. The results revealed that I 500 /I 670 of Ag 2 S CDs probes was linearly related to pH variation between pH 5.4 and 6.8. Meanwhile, the Ag 2 S CDs probes possessed a good reversibility along with pH changing between 5.0 and 7.0 without any interruption from common metal ions, proteins and other interferences.
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A carbon-based fluorescent nanoparticle is considered to be a new generation nontoxic nanoprobe suitable for various bioimaging and sensing applications. However, the synthesis of such a high-quality nanoparticle is challenging, and its application potential is mostly unexplored. Here we report a vitamin B1 carbonization-based approach for blue and green fluorescent carbon nanoparticles of <10 nm size with a fluorescence quantum of up to 76%. We found that carbonization of vitamin B1 in the presence of phosphate salt at ∼90-130 °C for about 2 h produces highly fluorescent carbon nanoparticles of 1-6 nm size. The particle size and fluorescence property can be controlled by varying the reaction temperature and nature of phosphate salt. Elemental analysis shows the incorporation of a large percentage (up to 48 wt %) of other elements (such as nitrogen, oxygen, phophorus, and sulfur) in the carbon matrix. The chemical structure of vitamin B1 (thiamine) is unique in a sense that it consists of a large number of heteroatoms along with unsaturated bonds and offers low-temperature carbonization with the formation of a nanoparticle having an optimum ratio of sp(2) and sp(3) carbon atoms. These carbon nanoparticles have high colloidal stability and stable fluorescence and have been used as fluorescent imaging probes.
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ABSTRACT In this article, a novel dual-color quantum dot–encoded fluorescent nanoprobe was prepared by the reverse microemulsion method and layer-by-layer assembly method. First, red fluorescence–emitting CdTe quantum dots were encapsulated in silica nanoparticles by the reverse microemulsion method. Yellow fluorescence–emitting quantum dots were deposited on the surface of silica nanoparticles to form a dual-color quantum dot@silica beads/quantum dot nanoprobe. Then capture DNA was linked to a QSQ nanoprobe via covalent bonding. We utilized the quantum dot@silica beads/quantum dot nanoprobe to capture and detect the mutant BRAF DNA sequence through the competitive immunoassay method. The resulting quantum dot@silica beads/quantum dot nanoprobe-capture DNA conjugates showed sequence-specific hybridization with target DNA. Furthermore, a multispectral imaging system was utilized to distinguish the quantum dot optical code in the quantum dot@silica beads/quantum dot nanoprobe. The quantum dot@silica beads/quantum dot nanoprobe was used in human osteoblast-like HepG2 cell imaging. The proposed quantum dot@silica beads/quantum dot nanoprobe and decoding analysis method could be used for targeting imaging, biological assays, and early detection of cancer.
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Abstract The combination of fluorescent nanoparticles and specific molecular probes appears to be a promising strategy for developing fluorescent nanoprobes. In this work, L-cysteine (L-Cys) capped Fe 3 O 4 @ZnO core-shell nanoparticles were synthesized for the highly selective detection of Fe 3+ . The proposed nanoprobe shows excellent fluorescent property and high selectivity for Fe 3+ due to the binding affinity of L-Cys with Fe 3+ . The binding of Fe 3+ to the nanoprobe induces an apparent decrease of the fluorescence. Thus a highly selective fluorescent chemosensor for Fe 3+ was proposed based on Fe 3 O 4 @ZnO nanoprobe. The magnetism of the nanoprobe enables the facile separation of bound Fe 3+ from the sample solution with an external magnetic field, which effectively reduces the interference of matrix. The detection limit was 3 nmol L −1 with a rapid response time of less than 1 min. The proposed method was applied to detect Fe 3+ in both serum and wastewater samples with acceptable performance. All above features indicated that the proposed fluorescent probe as sensing platform held great potential in applications of biological and analytical field.
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Cadmium telluride photovoltaics
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Ratiometric fluorescent probes have shown great potential for optical sensing and have been widely used for bioimaging. However, due to extremely stringent molecular design, most conventional organic ratiometric fluorescent probes can only achieve one single ratio. Currently, the detection of a target in living systems in a multiratiometric manner is prominently challenging. Therefore, it is of great significance to develop a method to construct the multiratiometric fluorescent probes for accurate quantitative detection of specific small molecules. To address this challenge, we developed a unique multiratiometric composite fluorescent probe. Herein, a new triple-ratiometric fluorescent composite nanoprobe CP@GQDs–OH based on OH-functionalized GQDs (GQDs–OH) and red emission ratiometric fluorescent molecular probe (CP) was prepared through π–π stacking. The novel nanoprobe showed high selectivity for SO2 with three linear ratio changes. Significantly, CP@GQDs–OH was successfully applied for the detection of SO2 in the living cells and zebrafishes by the unprecedented triple-ratiometric fashion.
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Бұл зерттеужұмысындaКaно моделітурaлы жәнеоғaн қaтыстытолықмәліметберілгенжәнеуниверситетстуденттерінебaғыттaлғaн қолдaнбaлы (кейстік)зерттеужүргізілген.АхметЯссaуи университетініңстуденттеріүшін Кaно моделіқолдaнылғaн, олaрдың жоғaры білімберусaпaсынa қоятынмaңыздытaлaптaры, яғнисaпaлық қaжеттіліктері,олaрдың мaңыздылығытурaлы жәнесaпaлық қaжеттіліктерінеқaтыстыөз университетінқaлaй бaғaлaйтындығытурaлы сұрaқтaр қойылғaн. Осы зерттеудіңмaқсaты АхметЯсaуи университетіндетуризмменеджментіжәнеқaржы бaкaлaвриaт бaғдaрлaмaлaрыныңсaпaсынa қaтыстыстуденттердіңқaжеттіліктерінaнықтaу, студенттердіңқaнaғaттaну, қaнaғaттaнбaу дәрежелерінбелгілеу,білімберусaпaсын aнықтaу мен жетілдіружолдaрын тaлдaу болыптaбылaды. Осы мaқсaтқaжетуүшін, ең aлдыменКaно сaуaлнaмaсы түзіліп,116 студенткеқолдaнылдыжәнебілімберугежәнеоның сaпaсынa қaтыстыстуденттердіңтaлaптaры мен қaжеттіліктерітоптықжұмыстaрaрқылыaнықтaлды. Екіншіден,бұл aнықтaлғaн тaлaптaр мен қaжеттіліктерКaно бaғaлaу кестесіменжіктелді.Осылaйшa, сaпa тaлaптaры төрт сaнaтқa бөлінді:болуытиіс, бір өлшемді,тaртымдыжәнебейтaрaп.Соңындa,қaнaғaттaну мен қaнaғaттaнбaудың мәндеріесептелдіжәнестуденттердіңқaнaғaттaну мен қaнaғaттaнбaу деңгейлерінжоғaрылaту мен төмендетудеосытaлaптaр мен қaжеттіліктердіңрөліaйқын aнықтaлды.Түйінсөздер:сaпa, сaпaлық қaжеттіліктер,білімберусaпaсы, Кaно моделі.
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