Pro-urokinase up-regulates the expression of urokinase-type plasminogen activator (u-PA) in human pulmonary arterial endothelial cells
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Objective: To explore the expression of urokinase-type plasminogen activator,(uPA),its receptor(uPAR,)their mRNA in hepatic carcinoma,its effects and mechanism.Methods: The expression of uPA and uPAR with their mRNA in 71 specimens of human hepatic tissue,including C 12,HCC 34 and PTT 25 were detected with immunohistochemistry and RT-PCR.The expression of uPA and uPAR and its mRNA correlating with clinical and pathological significance were compared and analysed.Results: The uPA and uPAR protein with their mRNA in HCC were significantly higher than that in C and in PTT(all P0.01);there was significant difference between PTT and C(all P0.05).Compared by the expression of uPA and uPAR protein with their mRNA in HCC with capsule invaded,intrahepatic and extrahepatic metastasis,there were significant higher than those integral capsule,without intrahe-patic and without extrahepatic metastasis(P0.05 or P0.01,)these expression was not significant correlation with the magnitude of HCC(all P0.05),with lower of Edmondson grade,they were higher stepup,but were not statistically significant.Conclusion: The uPA and uPAR show strongly expression in HCC and they may play significant roles in the generation,development,invasion and metastasis of HCC.
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The urokinase‐type plasminogen activator (uPA) and its inhibitor PAI‐2 form a covalent complex that, upon binding to the uPA receptor (uPA‐R), is cleaved into two fragments of molecular masses 70 kDa and 22 kDa. The 70‐kDa fragment results from the interaction of the B chain of uPA and PAI‐2 whereas the 22‐kDa fragment is the A chain of the enzyme [13]. We prove that, at 37°C, the 70–kDa fragment is released into the medium, whereas the 22–kDa fragment remains bound to the cell surface. uPA complexed with its other specific inhibitor, PAI‐1, is cleaved into fragments of identical sizes, but the 70–kDa component is internalized via the a 2 ‐macroglobulin receptor. At 4°C, both uPA/PAI‐2 complex degradation products remain bound to the uPA‐R. We propose that the 70–kDa molecule, which lacks the uPA binding region for uPA‐R, is bound to uPA‐R via a new binding site, unmasked only when uPA‐R is occupied by uPA/PAI‐2 complexes.
Plasminogen activator inhibitor-1
Cleavage (geology)
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Objective To observe the relation between estradiol and urokinase type plasminogen activator receptor (uPAR) of human hepatoma cells BEL- 7402. Methods The hepatoma cells were treated with varies contentions estradiol ,incubating 24h,48h and 72h. The expression of uPAR were detected using the polyclonal antibody by S- P immunohistochemistry. Results The expression of uPAR after the treatment of estradiol were higher than the control group (P0.05),showing time- dependant and concentration- dependant relationship. Conclusion Estradiol can effectively enhance the expression of uPAR.
Polyclonal antibodies
SuPAR
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Local and systemic changes in hemostasis are associated with allergic diseases. Apart from their well documented role in regulation of plasminogen activation, components of the urokinase system may be involved in modulation of cellular activities during immune inflammatory responses. So far, little has been known about the function of the system in allergic inflammation. In the present study, we assessed circulating levels of the urokinase system components such as urokinase-type plasminogen activator (uPA), soluble form of uPA receptor (CD87), and the inhibitor--plasminogen activator inhibitor type 1. The study comprised of patients suffering from allergic rhinitis, however, without any asthmatic symptoms. Plasma levels of uPA and soluble form of uPA receptor antigens, and plasminogen activator inhibitor type 1 activity were measured in 17 patients with grass pollens-induced intermittent allergic rhinitis and 15 patients with persistent allergic rhinitis due to house dust mite allergy, as well as in 20 sex-matched and age-matched healthy nonatopic participants. We did not observe any statistically significant differences in the levels of the urokinase system components between patients with intermittent allergic rhinitis and persistent allergic rhinitis, and the controls. The circulating levels of uPA, its soluble receptor, and its inhibitor did not differ between allergic rhinitis patients and healthy participants, therefore it seems that the systemic release and activity of the urokinase system molecules may be not significantly changed in the course of nasal allergic inflammation induced by periodic or continuous exposure to a natural allergen.
Allergic Inflammation
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OBJECTIVE To study the effect of vacuum-assisted closure (V.A.C) on the expression of Urokinase-type plasminogen activator (uPA) and Urokinase-type plasminogen activator receptor(uPAR) protein in margin tissue of pigs with acute wounds and patients with chronic wounds. METHODS Acute wounds were created on the two side of five male pigs' back, the experiment wounds on one side received V. A. C treatment and the control side received traditional treatment. Punch biopsies were taken from margin tissue of the wounds in 0, 1, 3, 6, 9, 12, 18, 25 days after the V.A.C treatment. The uPA and uPAR positive cells were stained with immunohistochemical technique . Six human chronic wounds were also treated with the V. A. C treatment, and the samples of extravasate from those wounds were collected in 0, 1, 3, 5, 7 days after the treatment, and the levels of uPA and uPAR expression were examined by enzyme-linked immunosorbent assay (ELISA). RESULTS The expression of uPA and uPAR protein in margin tissues of pigs with acute wounds increased and peaked in 3 days after the treatment with V. A. C, then it presented rapidly downtrend, but the expression and staining in the experiment group were obviously higher than that of the control group. In the six chronic wounds, the high level expression of uPA and uPAR protein was decreased after the treatment with V. A. C. CONCLUSION The V. A. C may increase the expression of uPA and uPAR protein in acute wound keratinocytes and decrease the high expression of uPA and uPAR in chronic wounds.
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Cell surface receptor
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A431 cells
Epidermoid carcinoma
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Cell surface receptor
Cell membrane
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Objective To study the expression of urokinase type plasminogen activator and its receptor in gliomas, and analysis the relationship between uPA/uPAR expression and the grade of human gliomas. Methods In surgical sample of 49 patients with glioma, 3 glioma cell lines and 12 samples of normal brain tissues obtained from craniocerebral injury surgery, the level of uPA/uPAR mRNA were studied using semi quantitative reverse transcription polymerase chain reaction. Results Along with the rise of glioma grade, the uPA mRNA and uPAR mRNA expression rate and level were also upregulated. U251 and other 2 glioma cells had a high level of uPA and uPAR mRNA, but the rate and level of uPA and uPAR mRNA of normal brain tissues were extremely lower. Conclusions The plasminogen system activation was quite higher in gliomas. The high level of uPA and uPAR mRNA reflecte a malignant biological behavior of glioma cells.
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