Optimization of multiplexed PCR on an integrated microfluidic forensic platform for rapid DNA analysis
33
Citation
47
Reference
10
Related Paper
Citation Trend
Abstract:
This study reports the design, prototyping, and assay development of multiplexed polymerase chain reaction (PCR) on a plastic microfluidic device. Amplification of 17 DNA loci is carried out directly on-chip as part of a system for continuous workflow processing from sample preparation (SP) to capillary electrophoresis (CE). For enhanced performance of on-chip PCR amplification, improved control systems have been developed making use of customized Peltier assemblies, valve actuators, software, and amplification chemistry protocols. Multiple enhancements to the microfluidic chip design have been enacted to improve the reliability of sample delivery through the various on-chip modules. This work has been enabled by the encapsulation of PCR reagents into a solid phase material through an optimized Solid Phase Encapsulating Assay Mix (SPEAM) bead-based hydrogel fabrication process. SPEAM bead technology is reliably coupled with precise microfluidic metering and dispensing for efficient amplification and subsequent DNA short tandem repeat (STR) fragment analysis. This provides a means of on-chip reagent storage suitable for microfluidic automation, with the long shelf-life necessary for point-of-care (POC) or field deployable applications. This paper reports the first high quality 17-plex forensic STR amplification from a reference sample in a microfluidic chip with preloaded solid phase reagents, that is designed for integration with up and downstream processing.Keywords:
Lab-on-a-Chip
Cartridge
Sample Preparation
Merge (version control)
Lab-on-a-Chip
Flow Control
Feed forward
Feedback Control
Cite
Citations (6)
Most microfluidic processes in lab-on-a-chip devices are electrokinetic processes. Fundamental understanding of the electrokinetic based microfluidic processes is key to the design and process control of lab-on-a-chip devices. This paper will review basics of the electrical double layer field, and three key on-chip microfluidic processes: electroosmotic flow, sample mixing and sample dispensing.
Lab-on-a-Chip
Microfluidic chip
Cite
Citations (0)
Sample preparation turns out to be one of the important procedures in complex sample analysis by affecting the accuracy, selectivity, and sensitivity of analytical results. However, the majority of the conventional sample preparation techniques still suffer from time‐consuming and labor‐intensive operations. These shortcomings can be addressed by reforming the sample preparation process in a microfluidic manner. Inheriting the advantages of rapid, high efficiency, low consumption, and easy integration, microfluidic sample preparation techniques receive increasing attention, including microfluidic phases separation, microfluidic field‐assisted extraction, microfluidic membrane separation, and microfluidic chemical conversion. This review overviews the progress of microfluidic sample preparation techniques in the last 3 years based on more than 100 references, we highlight the implementation of typical sample preparation methods in the formats of microfluidics. Furthermore, the challenges and outlooks of the application of microfluidic sample preparation techniques are discussed.
Sample Preparation
Sample (material)
Cite
Citations (9)
Cartridge
Cite
Citations (2)
A nozzle plate cartridge for the extraction section and a mixed plate cartridge containing both stainless steel and fluorothene sieve plates for the scrub section were developed. In 3- and 4-in.-diameter glass columns, these cartridges have a urnnium-extraction efficiency as good as, or better than, the present cartridge; they have a scrubbing efficiency, based on the transfer of chloride ion, which is about the same as the present cartridge. (auth)
Cartridge
Data scrubbing
Sieve (category theory)
Inert
Cite
Citations (0)
Aluminum cartridge case has long been considered as the alternative material for brass cartridge. It can efficiently reduce the weight carried by soldiers, help soldiers carry more ammunition with the same cartridge case, and thus improve the combat effectiveness. However, Aluminum cartridge case can be broken off during the course of use, which is a common phenomenon. By using the finite-element method, the paper is intended to analyze the effects on the deformation of cartridge case, the position and magnitude of maximum strain with the headspace variation law ;the maximum headspace of aluminum cartridge; the simulation result that maximum headspace indeed causes break is also verified by the firing experiment.
Cartridge
Ammunition
Brass
Cite
Citations (3)
In order to improve cartridge valve response speed,Working principle of cartridge valve is introduced,and the model of cartridge valve is simulated by AMESim software,drawing conclusions to improve cartridge valve response speed.The results show that stroke of main cartridge valve and the damping between main cartridge calve and pilot-operated valve effect hard to dynamic properties of cartridge valve;and closure time of main cartridge valve has been shorten from 0.341 s to 0.087 s,the effect of optimization is evidential,and have some guidance to improve response speed of cartridge valve.
Cartridge
Cite
Citations (0)
Through similar simulation studying, by using micro-and macro-observation method, analyzing the blasting effect of complex slotted cartridge and conventional slotted cartridge, obtain compound slotted cartridge better directional blasting effect and higher energy efficiency, and less damage to the surrounding rock, more suitable for smooth wall blasting.
Cartridge
Cite
Citations (1)
Sample preparation has long been recognized as a significant barrier to the implementation of macroscopic protocols on microfabricated devices. Macroscopically, such tasks as removing salts, primers and other contaminants are performed by methods involving precipitation, specialized membranes and centrifuges, none of which are readily performed in microfluidic structures. Although some microfluidic systems have been developed for performing sample purification, their complexity may hinder the degree to which they can be implemented. We present a method of microchip-based sample purification that can be performed with even the simplest microfluidic designs. The technique is demonstrated by removing primers from a sample of amplified DNA, leaving only the product DNA. This provides a new sample preparation capability for microfluidic systems.
Sample Preparation
Sample (material)
Cite
Citations (32)