Comparative profile of paddy straw pretreated with standard and isolated lignocellulolytic fungal cultures
5
Citation
11
Reference
20
Related Paper
Citation Trend
Abstract:
The ligno-cellulosic biomass is the most abundant and also the most renewable biomaterial on earth. The development of alternative energy technology such as bioconversion of biomass is critically important because of the rising prices of crude oil, security issues regarding the oil supply and air pollution. Many microorganisms in nature are able to attack and degrade lignin, thus making access to cellulose easy. Such organisms are abundantly found in forest leaf litter/composts and especially include the wood rotting fungi, actinomycetes and bacteria. These microorganisms possess enzyme systems to attack, depolymerize and degrade the polymers in lignocellulosic substrates. Fungi such as Trichoderma reesei and Trichoderma harzianumproduce large amounts of extracellular cellulolytic enzymes, whereas higher fungi such as basidiomycetes (e.g. Phanerochaete chrysosporium, Coriolus versicolor, Pleurotus ostreatus, Fusarium sp.) have unique oxidative systems which together with ligninolytic enzymes are responsible for lignocellulose degradation. These lignocellulolytic fungi can prove extremely useful in delignification of lignocellulosic biomass. The present work reports the comparison in chemical compostion of paddy straw pretreated with Coriolus versicolor MTCC 138 (standard) andFusarium sp. (isolated from compost/digested slurry/ plant debris). Lignin loss observed was 27.1 and 17.5% on 20th day in paddy straw pretreated with C. versicolor MTCC 138 and Fusarium sp., respectively. Thus, fungal pretreatment for lignocellulosic substrate can be developed to facilitate efficient degradation of lignocellulosic biomass.
Key words: Delignification, fungal pretreatment, lignocellulosic biomass.Keywords:
Lignocellulosic Biomass
Pleurotus
Trametes versicolor
Bioconversion
Trichoderma
Pleurotus ostreatus
Hemicellulose
Pleurotus
Cite
Citations (72)
Pleurotus ostreatus
Cite
Citations (0)
In Europe, the agricultural wastes represent a significant potential for the development of biorefineries in different sectors such as cereals. Recovery of phytochemicals as well as the energetic valorization of the plant matrixes needs the demolition of the wall cell plants. Hydrolitic demolition by lignocellulosic enzymes is one of the most studied approach. White rot fungi such as Pleurotus ostreatus produce a wide range of extracellular enzymes to degrade complex lignocellulosic substrates into soluble substances that can be used as nutrients.The objective of this study is to induce the production of lignocellulosic enzymes through the growth Pleurotus ostreatus in solid state fermentation using agro-food wastes as substrates: rice husks and wheat straw. The activities of cellulase, xylanase, peroxidase, laccase, and arylesterase are determinate by specific colorimetric assays.All trials showed a scarce productivity of cellulase while all the detected enzymatic activities resulted higher using rice husk as a substrate than in presence of wheat straw. Pleurotus ostreatus exhibited a prevalent production of arylesterase activity and, in particular, the contemporary presence of significant xylanase and feruloilesterase activities was probably due to the typical ferulic bond and diferulic bridge in the heteroxylane structure of monocot’s plant cell walls such as rice and wheat. Moreover, in terms of yields arylesterase activities for both substrates, are prevalent on other activities.Our findings showed that the enzymatic production was strictly dependent to the periodic removal of the produced enzymes. The development of new solid state bioreactor design for a steady state production of enzymes from Pleurotus ostreatus could open an interesting industrial approach.
Husk
Solid-State Fermentation
Rice straw
Cite
Citations (13)
Three white-rot fungi: Daedalea elegans, Polyporus giganteus, and Lenzites betulina were screened for their lignin degrading abilities on rice straw, maizecob, sawdust of Terminalia superba, and sugarcane bagasse at different time intervals (30, 60, and 90 days). All the fungi demonstrated varying levels of ligninolytic capability with different degrees of lignin degradation in all the fermented substrates. A significant difference (p<0.05) was observed in the mycelia extension of Daedalea elegans grown on the different agro-industrial wastes. D. elegans gave maximum extension of 4.5 cm on sugarcane bagasse. The highest lignin reduction of 92.9% (p<0.05) was recorded in maize cob fermented with Daedalea elegans after 90 days. On the basis of lignocellulosic material degraded, it is concluded that the white-rot fungi offer a better alternative to conventional ways of disposing these waste substances. This paper considers the ability of indigenous white-rot fungi to degrade lignin as a way of using them in effective waste management.
White rot
Bagasse
Corncob
Sawdust
Polyporus
Cite
Citations (9)
The crops straw is an important biological resource. Because of its particular composition and structure, the hardly-degradation is always the problem of straw conversion and application. So Attention is paid to the desradation and convertion of the straw into other useful materials.According to the PDA plate culture, shake-flask culture and solid-state fermentation with straw,the best strain which had growth advantage and yielded high activity lignin-degrading enzymes was chosen from 6 common white-rot funguses. In order to degrade and utilize the straw obviously,the two-step mixed fermentation by Pteurorus ostreatus and Trichoderma koningii was used. Through the different combination ways,H6-T10 group can get the best straw degradation result. The lignin degradation rate reached 44.77%, and the cellulose degradation rate was 41.48%.
Solid-State Fermentation
Degradation
Cite
Citations (3)
Lignocellulose is the most abundant natural polymer on earth. It is the main agro-industrial waste component, it has no added value, and in some cases is the main cause of environmental problems. The fungi can be used in the transformation of residues and different biotechnological processes. In the present research, seven strains of white rot fungi collected in Ibague (Colombia) were isolated. The production of ligninolytic and cellulolytic enzymes as well as their capacity to transform lignocellulose from banana peel, rice husk and cocoa pod husk were evaluated. Strains of Pycnoporus sanguineus, Hexagonia glabra and Lentinus crinitus were selected from the enzymatic screening and showed high production of ligninolytic enzymes. The evaluation of agricultural waste degradation was carried out by Fourier-transform infrared spectroscopy and the results showed the ability of the tested fungi to, preferentially, degrade lignin or cellulose in each substrate. Based on the results, different biotechnological potentialities for each strain were proposed.
White rot
White (mutation)
Cite
Citations (0)
Stalk
Pleurotus
Lignocellulosic Biomass
Banana peel
Bioconversion
Pleurotus ostreatus
Cite
Citations (64)
Pleurotus ostreatus
Pleurotus
Phanerochaete
Cite
Citations (79)
Cite
Citations (2)
Pleurotus
Trametes versicolor
Phanerochaete
Corn stover
Lignocellulosic Biomass
Stover
Cite
Citations (75)