Isoelectric points of proteins, determined by isoelectric focusing in the presence of urea and ethanol

This experiment used isoelectric focusing electrophoresis(IEF)and HPLC methods, through the determination of the rhIGF-1 isoelectric point as an example, established a rapid determination of protein isoelectric point method. The gel was 7.5% of the gel concentration, 3% of the crosslinking degree, and 6% of the Amoholine, and determined the isoelectric point of rhIGF-1 by the Alpha imager TM 2200 and HPLC. The results showed that the isoelectric point of rhIGF-1 was 8.20.The pH gradient curve linear regression equation was Y=0.0664X+3.8217, The correlation coefficient r was 0.9956. That was mean the result was accurate.

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PI Mtoyoura: A new PI M subtype found by separator and hybrid isoelectric focusing

Electrophoresis (1988)

Isao Yuasa Kazuyuki Suenaga Kazuo Umetsu Osamu Inagaki Jun Ikebuchi

In the course of a population study of alpha 1-antitrypsin polymorphism by separator isoelectric focusing, a variant phenotype having a somewhat narrower spacing than PI M1M3 was observed in a Japanese blood donor. Family studies by hybrid isoelectric focusing in a carrier ampholyte-supplemented immobilized pH gradient from 4.35-4.65 revealed that the products of the responsible gene, PI*Mtoyoura, were extremely close but slightly cathodal to those of PI*M1. The difference in isoelectric point between them corresponded to the resolving limit of isoelectric focusing. For this reason, although the propositus' father was deduced to have the genotype PI*M1/PI*Mtoyoura, the products of these two genes failed to form a double band pattern. Thus, the gene frequency for PI*Mtoyoura was unknown. These findings, however, indicate that a further microheterogeneity in the PI M subtype exists at least in the Japanese.

Separator (oil production)

10.1002/elps.1150090311

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Isoelectric points and conformation of proteins

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics (1971)

Nobuo Ui

10.1016/0005-2795(71)90273-x

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Isoelectric focussing of normal human erythrocyte pyruvate kinase (PK) and PK variants with abnormal electrophoretic patterns

Clinica Chimica Acta (1976)

Etsuko Oda Koji Nakashima Kenji Shinohara Shiro Miwa

10.1016/0009-8981(76)90407-1

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Isoelectric points of proteins, determined by isoelectric focusing in the presence of urea and ethanol

Journal of Chromatography A (1979)

W.J. Gelsema C.L. De Ligny N.G. van der Veen

10.1016/s0021-9673(01)95297-5

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Citations (53)

General Aspects of Isoelectric Focusing of Proteins

Protides of the biological fluids (1970)

Olof Vesterberg

10.1016/b978-0-08-015566-1.50065-4

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Distribution ofα 1 phenotypes in Japanese: Description of Pi M subtypes by isoelectric focusing

The Japanese Journal of Human Genetics (1979)

Kazuhiko Miyake Hiroshi Suzuki Hiroshi Oka Toshitsugu Oda Shoji Harada

10.1007/bf01888921

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STUDY ON THE ISOELECTRIC POINT OF LOW TEMPERATURE INDUCED PROTEIN OF STRAW MUSHROOM MEASURED BY ISOELECTRIC FOCUSING ELECTROPHORESIS

Journal of Zhengzhou Grain College (2000)

An Zhao

The isoelectric point of low temperature induced protein of straw mushroom was measured by isoelectric focusing electrophoresis with wide range pH3～10 and narrow range pH5～8 respectively.The isoelectric piont of the protein is 6.79,and the coefficients r of pH gradient curve is 0.937.

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