Tobacco leaf veins as sources of a cucumber mosaic virus for aphids
0
Citation
4
Reference
10
Related Paper
Keywords:
Polyclonal antibodies
Tobacco etch virus
Tobacco mosaic virus (TMV) and Cucumber mosaic virus expressing green fluorescent protein (GFP) were used to probe the effects of salicylic acid (SA) on the cell biology of viral infection. Treatment of tobacco with SA restricted TMV.GFP to single-epidermal cell infection sites for at least 6 d post inoculation but did not affect infection sites of Cucumber mosaic virus expressing GFP. Microinjection experiments, using size-specific dextrans, showed that SA cannot inhibit TMV movement by decreasing the plasmodesmatal size exclusion limit. In SA-treated transgenic plants expressing TMV movement protein, TMV.GFP infection sites were larger, but they still consisted overwhelmingly of epidermal cells. TMV replication was strongly inhibited in mesophyll protoplasts isolated from SA-treated nontransgenic tobacco plants. Therefore, it appears that SA has distinct cell type-specific effects on virus replication and movement in the mesophyll and epidermal cell layers, respectively. Thus, SA can have fundamentally different effects on the same pathogen in different cell types.
Cucumovirus
Movement protein
Protoplast
Tobamovirus
Plasmodesma
Cite
Citations (115)
Four isolates of tobacco mosaic virus (TMV) were purified from the egg-plant,pepper,tomato and tobacco plant growing in the suburb of Shang-hai and Guang-Zhou. The results of the comparative studies on the relationships of the se-rology,molecular wight of coat pro-tein sub-unit,amino acid composi-tion as well as ~(125)I-labled peptide mapping of coat protein showed thatthe four isolates are the member or strains of the tobomoviruses with different ~(125)I-peptide mapping of the coat protein from that of the common of TMV and that of the Shang-hai of ribgrass mosaic virus. The autoradiogram of the peptide mapping of the coat protein is useful for the identification of the member or strain of TMV.
Strain (injury)
Tobacco leaf
Cite
Citations (0)
Tobacco mosaic virus (TMV) and Cucumber mosaic virus expressing green fluorescent protein (GFP) were used to probe the effects of salicylic acid (SA) on the cell biology of viral infection. Treatment of tobacco with SA restricted TMV.GFP to single-epidermal cell infection sites for at least 6 d post inoculation but did not affect infection sites of Cucumber mosaic virus expressing GFP. Microinjection experiments, using size-specific dextrans, showed that SA cannot inhibit TMV movement by decreasing the plasmodesmatal size exclusion limit. In SA-treated transgenic plants expressing TMV movement protein, TMV.GFP infection sites were larger, but they still consisted overwhelmingly of epidermal cells. TMV replication was strongly inhibited in mesophyll protoplasts isolated from SA-treated nontransgenic tobacco plants. Therefore, it appears that SA has distinct cell type-specific effects on virus replication and movement in the mesophyll and epidermal cell layers, respectively. Thus, SA can have fundamentally different effects on the same pathogen in different cell types.
Cucumovirus
Movement protein
Protoplast
Tobamovirus
Plasmodesma
Cite
Citations (12)
The inhibition of plant activator protein on RNA and coat protein of tobacco mosaic virus(TMV) was studied.The concentration of TMV RNA in the treated tobacco was reduced by 28%.Using ELISA and Western blotting assays,the concentration of TMV coat protein in the treated tobacco was reduced by 20.7% and 25.0% respectively.It was also revealed that the polymerization of TMV coat protein could be interfered distinctly by plant activator protein at 22-37℃ in vitro.
Tobamovirus
Cite
Citations (1)
The process of modification of tobacco mosaic virus (TMV) coat protein with a lysine-specific reagent trinitrobenzenesulfonic acid (TNBS) was studied. TMV coat protein molecule is known to contain just two Lys residues (K53 and K68) localized in the same region of TMV coat protein subunit tertiary structure at a distance of about 70Ao from the virion axis. TNBS was used to modify the coat protein of wild type (U1) TMV and that of a coat protein ts-mutant ts21-66, bearing two amino-acid substitutions (I21 T and D66 G), both localized in the 70Ao region. In the mutant coat protein, TNBS modification rate for one of the two Lys residues was found to increase drastically (7 to 8 times) between 30 and 32o at pH 8.0. In U1 coat protein a similar increase was observed for one of Lys residues at 36o and pH 8.6. The results may indicate that the 70Ao region represents the labile section of TMV coat protein molecule, and gradual destruction of this site results first in loss of protein capacity to form well arranged two-cylindrical aggregates and then (after slight heating) in loss of capacity to form ordered helical aggregates.
Coat
Alfalfa mosaic virus
Cite
Citations (0)
Strain (injury)
Coat
Tobamovirus
Cite
Citations (0)
101 virus samples were collected in tobacco producing regions in Xianyang City and Shangzhou City of Shaanxi province in 2001. Five viruses were identified by biological determination, serological assay (Double diffusion and Enzymelinked immiunosorbbent assay) and microscopy observation. They were tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), tobacco etch rirus (TEV), patato virus Y (PVY) and tobacco ringspot virus (TRSV). Tobacco mosaic virus (TMV), cucumber mosaic virus (CMV) and tobacco etch rirus (TEV) were the most common viruses and caused serious losses in fields. Simultaneously the complex infection of tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV), patato virus Y (PVY) and tobacco mosaic virus(TMV) are also a crucial problem to be resolved at present.
Tobacco etch virus
Potato virus Y
Cite
Citations (0)
Cucumovirus
Viral Interference
Tobamovirus
Mosaic virus
Cite
Citations (4)
Mosaic virus
Tobacco leaf
Coat
Mosaic
Alfalfa mosaic virus
Cite
Citations (2)
Cucumber mosaic virus (CMV) is one of the most important plant viruses and a major threat to a wide range of hosts. Prevalence of CMV in Pakistan is alarming for vegetable production especially cucurbits. The present study was done to estimate the prevalence, distribution as well as coat protein base identification of this notorious virus. During 2015-16 incidence of CMV was recorded in cucumber field in the Pothwar region of Pakistan (Rawalpindi, Attock, Jhelum, Chakwal, and Islamabad). During survey 150 samples were collected and tested through DASELISA (Double Antibody Sandwiched Enzyme Linked Immunosorbent Assay). Results show that CMV prevails throughout the region. Maximum disease incidence was recorded in Rawalpindi (50%) followed by Chakwal (46%), Attock (43%), Islamabad (40%) and Jhelum (36%). Virus infectivity was assayed by indicator plants (Capsicum annuum, Cucumis sativus cv, Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum, and Datura stramonium) through mechanical inoculation. Upon mechanical inoculation, plants show Chlorotic lesion, Necrotic lesion, Mosaic, Stunting, Spots. Coat protein (CP) gene-specific forward (CMVF-45) and reverse (CMVR-45) primer amplified 500bp fragments through Polymerase Chain Reaction (PCR).
Cucumis
Cucumovirus
Coat
Identification
Cite
Citations (1)