META060 attenuates TNF-α-activated inflammation, endothelial–monocyte interactions, and matrix metalloproteinase-9 expression, and inhibits NF-κB and AP-1 in THP-1 monocytes
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Monocyte
THP1 cell line
Matrix metalloproteinase 9
Proinflammatory cytokine
Aim To investigate the effects of cerivastatin on expression of matrix metalloproteinase (MMP-9) and CD40 in THP-1 cells. Methods THP-1 cells were incubated with increasing concentration of cerivastatin. MMP-9 and CD40 mRNA were detected by RT-PCR, MMP-9 concentration in media were assayed by ELISA. Results Cerivastatin inhibited the expression of CD40 and MMP-9 concentration dependently. At 0.01 μmol/L, the mRNA levels of CD40 and MMP-9 were not significantly decreased. But expression of CD40 and MMP-9 mRNA were significant down-regulated at 1 to 10 μmol/L; Level of MMP-9 concentration in media decreased from 0.469±0.06 to 0.243±0.04 and 0.039±0.01 respectively at 1 or 10 μmol/L(P0.05). Conclusions Cerivastatin inhibited the expression of CD40 and MMP-9 in THP-1 cells, decrease the MMP-9 concentration in media, it suggest that HMG-CoA reductase inhibitors may suppress inflammatory signaling and plaque disruption which responsible for clinical acute coronary syndrome.
Cerivastatin
THP1 cell line
Matrix metalloproteinase 9
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Matrix metalloproteinases (MMPs) are also known as matrixins.Many diseases,such as osteoarthritis,multiple sclerosis,and cancer,are accompanied by changes of activity of MMPs.MMPs play roles in invasion and metastasis of malignant tumors.This article reviews the status and development on relationship between MMP-2,MMP-9 and the occurrence and metastasis of lung cancer.
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Matrix metalloproteinases; Lung cancer; Matrix metalloproteinase-2; Matrix metalloproteinase-9
Matrix metalloproteinase 9
Matrix (chemical analysis)
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Monocyte
Ropivacaine
Matrix metalloproteinase 9
Matrix (chemical analysis)
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Abstract The authors have withdrawn this preprint due to erroneous posting.
THP1 cell line
Inflammatory response
Proinflammatory cytokine
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Matrix metalloproteinase 9
Matrix metalloproteinase inhibitor
Matrix Metalloproteinase 3
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THP1 cell line
Monocytic leukemia
Line (geometry)
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THP1 cell line
Monocytic leukemia
Acute monocytic leukemia
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TSUCHIYA, S., MINEGISHI, N., MINEGISHI, M., SATO, T. and KONNO, T. Adaptation of a Human Monocytic Leukemia Cell Line (THP-1) in a Protein- Free Chemically Defined Medium. Tohoku J. exp. Med., 1986, 150 (4), 455-465 -We have adapted a human monocytic leukemia cell line [THP-1c12(+)] so that it can proliferate in a completely protein-free chemically defined medium of an equal mixture of Dulbecco's modified Eagle's minimum essential medium and Ham's F12. This cell line was designated as THP-1c12(-). When a sufficient number of THP-1c12(-) cells was seeded in culture, the maximum cell density after 6 days of culture was 1×106/ml with a doubling time of 30 hr. Transferrin showed a slight stimulative effect on the growth of THP-1c12(-) cells, but insulin did not. The surface profile of THP-1c12(-) was the same as that of THP-1c12(+), which possessed Mol. Mo5, LeuM3, My9 and Ia-like antigens as well as a large number of Fc receptors. Phagocytic ability with respect to IgG-coated sheep red blood cells was evident in both THP-1c12(+) and THP-1c12(-) cells. A small percentage of THP-1c12(-) cells retained the ability to reduce nitroblue tetrazolium when stimulated with 12-tetradecanoyl-phorbol-13-acetate. Culture supernatant from THP-1c12(-) stimulated the incorporation of 3H-thymidine into its own cells, suggesting an autocrine mechanism by which the growth of THP-1c12(-) cells is induced in a protein-free medium.
THP1 cell line
Monocytic leukemia
Acute monocytic leukemia
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THP1 cell line
Matrix metalloproteinase 9
Matrix (chemical analysis)
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