Chemical Optimization of Protein Extraction from Sweet Potato (Ipomoea batatas) Peel
19
Citation
31
Reference
10
Related Paper
Citation Trend
Abstract:
Abstract: Proteins isolated from sweet potatoes ( Ipomoea batatas ) have been shown to possess antidiabetic, antioxidant, and antiproliferative properties. The objective of this study was to chemically optimize a process for extracting proteins from sweet potato peel. The extraction procedure involved mixing peel with saline solvent to dissolve proteins and then precipitating with CaCl 2 . Quadratic and segmented models were used to determine the optimum NaCl concentration and peel to solvent ratio to maximize protein solubility while minimizing solvent usage. A segmented model was also used to optimize the concentration of CaCl 2 used for precipitation. The highest yield was obtained by mixing blanched peelings with 59.7 mL of 0.025 mM NaCl per g peel and then precipitating with 6.8 mM CaCl 2 . The results of this study show that potentially valuable proteins can be extracted from peel generated during processing of sweet potatoes and industrial costs can be minimized by using these optimum conditions. Practical Application: Potentially valuable proteins can be extracted from sweet potato peel, a waste product of sweet potato processing.Keywords:
Ipomoea
Protein isolate
We explain the concept of solubility synergistic solvation (also known as parabolic solubility), wherein a binary solvent mixture exhibits a higher solubility than either of the component solvents alone. In cases where a compound has poor solubility in pure solvents, a binary solvent mixture may be used to enhance its solubility; however, the obvious challenge is how to identify such solvent pairs efficiently and economically as the number of all possible binary solvent combinations is prohibitively large. After analyzing 33 879 pieces of compound solubility data that were collected from solvent mixtures, we conclude that synergistic solvation may be more common than generally appreciated, the magnitude of which oftentimes is significant enough to make the use of binary mixtures more advantageous than pure solvents. For charged solutes, we confirmed that adding a small amount of water to organic solvents would often improve their solubility substantially. As for noncharged solutes, we identified four "privileged solvent pairs" that are strongly synergistic. In this report, four case studies are discussed which illustrate how synergistic effects have been used to support chemical process development in different ways.
Hildebrand solubility parameter
Cite
Citations (29)
Using different concentrations of NAA to deal with 5 kinds of Ipomoea L.branch,to research the influence on 5 kinds of plants' survival rate,roots and buds.The results showed that Ipomoea cairica and Ipomoea obscura were hard to survive,another 3 plants' survival rate from high to low were Ipomoea pes-caprae,Ipomoea stolonifera,Ipomoea triloba.NAA had effect on the survival rate,roots and buds,and the most sensitive plant for NAA was Ipomoea triloba.
Ipomoea
Convolvulaceae
Ipomoea aquatica
Cite
Citations (0)
Ipomoea
Panama
Convolvulaceae
Cite
Citations (0)
The solubility of a drug depends upon the solvent. Hydrophilic drug are soluble in hydrophilic solvent while hydrophobic drugs are soluble in hydrophobic solvent, sometimes drugs contain both hydrophilic and hydrophobic part and single solvent that in sufficient to improve the solubility of drug is required. Frequently a soluble is more soluble in a mixture of solvents that in a single solvent alone, this phenomenon is known as co solvency and the solvent in combination increase the solubility of the solute is called the co solvents. The aim of present study was to study the effect of co solvents on the solubility of benzoic acid. From the graph it has been seen that the solubility of benzoic acid increases with the increased of concentration of co-solvent. When the straight line was extrapolated to Y axis, the intercept gives the solubility of benzoic acid in water. Key words: Solubility, poorly soluble drugs, benzoic acid, IPA, co solvents
Benzoic acid
Cite
Citations (0)
Ipomoea
Convolvulaceae
Convolvulus
Ipomoea aquatica
Cite
Citations (2)
The physicochemical characteristics offive sweet potato varieties were studied and compared to potato starch. The parameters studied were starch yield, amylose content, proximate content, swelling power and solubility and yneresis. The starch content offive sweet potato varieties ranged between 23.21Yrr 29.7% The Ipomoea batatas variety 4 gave the highest yield of starch (29.7%) while Ipomoea batatas variety 3 gave the lowest yield (23.2%). The amylose contents ranged between 20. J3lYrr 25.73% Amylose content of Ipomoe~ batatas variety 5 was the highest (25.73%) while starch from Ipomoea batatas showed the lowest amylose content (20.13%). The moisture contents ranged were 8. 761Yrr9. 05% The moisture content of Ipomoea batatas variety 4 was the highest (9.05%) and the lowest was Ipomoea batatas variety 1 (8.76%). The ash content varied between 0.47IYrrO.52% Ipomoea batatas variety 3 showed the highest ash content (0.52%) and the lowest ash content was found for Ipomoea batatas variety 4 (0.47%). The lipid content varied between o. 091YrrO. 12% Ipomoea batatas variety 2 gave the highest lipid content (0.12%) while the lowest was found for Ipomoea batatas variety 3 (0.09%). The protein contents were in the range of o. 171YrrO. 19% Ipomoea batatas variety 4 gave the highest protein content (0.19%) while Ipomoea batatas variety 1 gave the lowest protein content (0.17%). The swelling power ofIpomoea batatas variety 3 starch was the highest among all samples of sweet potato starches at all the temperature ranged studied while Ipomoea batatas variety 5 showed the lowest swelling power. For solubility, it was found that Ipomoea batatas variety 5 showed the
highest solubility and the lowest wasfoundfor Ipomoea batatas variety 3 at all the temperature ranged studied. Syneresis for Ipomoea batatas variety 5 starch was the highest while Ipomoea batatas had the lowest value ofsyneresis tendency among the sweet potato starches studied.
Ipomoea
Convolvulaceae
Cite
Citations (0)
Abstract Background As a major family of plant-specific transcription factors, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes play crucial regulatory roles in plant growth, development, and stress tolerance. SPL transcription factor family has been widely studied in various plant species, however, there are no systematic studies on SPL genes in genus Ipomoea . Results In this study, a total of 29, 27, 26, 23 SPL genes were identified in Ipomoea batatas , Ipomoea trifida , Ipomoea triloba , and Ipomoea nil , respectively. Phylogenetic analysis indicated that Ipomoea SPL genes could be clustered into eight clades. SPL members within the same clade showed similar gene structures, domain organizations, and cis-acting element compositions, suggesting similarity of biological function potentially. Evolutionary analysis revealed that segmental duplication events played a major role in the Ipomoea genus-specific expansion of SPL genes. Of these Ipomoea SPL genes, 69 were predicted as the target genes of miR156, and 7 IbSPL genes were further confirmed by degradome data. Additionally, IbSPL genes showed diverse expression patterns in various tissues, implying their functional conservation and divergence. Finally, by combining the information from expression patterns and regulatory sub-networks, we found that four IbSPL genes ( IbSPL16 / IbSPL17 / IbSPL21 / IbSPL28 ) may be involved in the formation and development of storage roots. Conclusions This study not only provides novel insights into the evolutionary and functional divergence of the SPL genes in all available sequenced species in genus Ipomoea , but also lays a foundation for further elucidation of the potential functional roles of IbSPL genes during storage root development.
Ipomoea
Functional divergence
Convolvulaceae
Cite
Citations (0)
As a major plant-specific transcription factor family, SPL genes play a crucial role in plant growth, development, and stress tolerance. The SPL transcription factor family has been widely studied in various plant species; however, systematic studies on SPL genes in the genus Ipomoea are lacking. Here, we identified a total of 29, 27, 26, and 23 SPLs in Ipomoea batatas, Ipomoea trifida, Ipomoea triloba, and Ipomoea nil, respectively. Based on the phylogenetic analysis of SPL proteins from model plants, the Ipomoea SPLs were classified into eight clades, which included conserved gene structures, domain organizations and motif compositions. Moreover, segmental duplication, which is derived from the Ipomoea lineage-specific whole-genome triplication event, was speculated to have a predominant role in Ipomoea SPL expansion. Particularly, tandem duplication was primarily responsible for the expansion of SPL subclades IV-b and IV-c. Furthermore, 25 interspecific orthologous groups were identified in Ipomoea, rice, Arabidopsis, and tomato. These findings support the expansion of SPLs in Ipomoea genus, with most of the SPLs being evolutionarily conserved. Of the 105 Ipomoea SPLs, 69 were predicted to be the targets of miR156, with seven IbSPLs being further verified as targets using degradome-seq data. Using transcriptomic data from aboveground and underground sweet potato tissues, IbSPLs showed diverse expression patterns, including seven highly expressed IbSPLs in the underground tissues. Furthermore, the expression of 11 IbSPLs was validated using qRT-PCR, and two (IbSPL17/IbSPL28) showed significantly increased expression during root development. Additionally, the qRT-PCR analysis revealed that six IbSPLs were strongly induced in the roots under phytohormone treatments, particularly zeatin and abscisic acid. Finally, the transcriptomic data of storage roots from 88 sweet potato accessions were used for weighted gene co-expression network analysis, which revealed four IbSPLs (IbSPL16/IbSPL17/IbSPL21/IbSPL28) clusters with genes involved in "regulation of root morphogenesis," "cell division," "cytoskeleton organization," and "plant-type cell wall organization or biogenesis," indicating their potential role in storage root development. This study not only provides novel insights into the evolutionary and functional divergence of the SPLs in the genus Ipomoea but also lays a foundation for further elucidation of the potential functional roles of IbSPLs on storage root development.
Ipomoea
Convolvulaceae
Cite
Citations (13)
Ipomoea
Convolvulaceae
Convolvulus
Ipomoea aquatica
Cite
Citations (0)
The effect of ultra high pressure(UHP)on solubility of isolated peanut protein was studied in this paper.The solubility of peanut protein were investigated under different condition of pressure, time,protein concentration,pH value.The results show that the solubility of isolated peanut protein is better with the higher treatment pressure and longer treatment time;The solubility of isolated peanut protein increased with the concentration and pH increase.
Protein isolate
Cite
Citations (1)