Antioxidant and anticancer activity of 3,5-dihydroxy-4-isopropylstilbene produced by Bacillus sp. N strain isolated from entomopathogenic nematode
Sasidharan Nishanth KumarBala NambisanB. Santhosh KumarNisha Girija VasudevanChellapan MohandasVino T. CheriyanRuby John Anto
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HeLa
Acridine orange
Hydroxyl radical
MTT assay
Objective To investigate the effect of SAHA(suberoylanilide hydroxamic acid)on p53 wild type HeLa cells and p53 knock-down HeLa-E3 cells.Methods The cell's proliferation were determined by MTT after 24,48 and 72 h incubation;the cell cycle distribution and cell death were detected by flow cytometry and typan-blue assay,respectively.Results SAHA significantly suppressed the in vitro proliferation of HeLa and HeLa-E3 cells in a time and dose dependent manner.G2/M arrest and cell death of HeLa cells were also elevated by SAHA in 24 h,while HeLa cells were more sensitive to SAHA than HeLa-E3 cells.Conclusion The results show that the sensitivity of tumor cells to SAHA were influenced by p53.
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Background: When diagnosing and treating male infertility it is important to determine whether there are defects in the maturation process of sperm nuclei. Using nutritional supplements can improve the morphological and physiological condition of the spermatozoa. In recent years there has been an increase in the usage of supplements with different compositions which strives to determine the best combination and avoid side effects. Aim: To study the effect of PAPA nutritional supplement on the levels of DNA fragmentation of sperm cells tested with acridine orange test (single stranded DNA against double stranded DNA) in men with sub/infertility. Materials and methods: 48 men with confirmed sub/infertility underwent treatment for three months with nutritional supplement PAPA containing 9 micronutrients. The differences in levels of DNA fragmentation were determined with acridine orange test, which was conducted before and after the treatment. Results: The results were statistically significant (p<0.001) showing an increase in the number of green spermatozoa (normal DNA), and a decrease of damaged ones (orange and red). After treatment the level of sperm DNA fragmentation decreased by 10.2%. Conclusion: Men with confirmed sub/infertility that took nutritional supplement PAPA for three moths showed a decrease in DNA fragmentation levels of 10.2% determined by AO test which implies an improvement of male fertility levels.
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Fragmentation
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Objective To investigate the apoptosis inducing effect of N-4-hydroxyphenyl retinode(4-HPR)and Cisplatin on human HeLa cell lines.Methods MTT assay was employed to examine the growth suppression of the cells.TEM was used to observe the ultrastructural change of HeLa cells treated with 4-HPR and Cisplatin.The apoptosis rate and change in cell cycle of HeLa cells treated with 4-HPR or/and Cisplatin were examined by flow cytometer.Results 4-HPR and Cisplatin both could induce apoptosis of HeLa cells;combined use of 4-HPR and Cisplatin could enhance HeLa cells apoptosis.Conclusion 4-HPR and Cisplatin could induce apoptosis of HeLa cells,and their effect is synergetic.
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[Objective]The purpose was to study the scavenging effects of Fructus Ulmns pumila water-extraction and ethanol-extraction on superoxide anion and hydroxyl free radical.[Method]Fructus ulmns pumila extractions were made to analyze the effect of extractions on scavening rate of superoxide anion and hydroxyl radical.[Result]The results showed that whether Fructus Ulmns pumila water-extraction or ethanol-extraction had scavenging effects on superoxide anion and hydroxyl radical.Fructus Ulmns pumila water-extraction had higher scavenging effects on superoxide anion than on hydroxyl radical,but Fructus Ulmns pumila ethanol-extraction had stronger scavenging effects on hydroxyl radical than on superoxide anion.[Conclusion]Fructus ulnms pumila water-extraction and ethanol-extraction have all the better ability of cleanning out hydroxyl radical and controlling superoxide anion.
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Scavenging
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Objective To observe the inhibition effect of the extract B of Polygonatum odoratum(EB-PAOA) on human cervix cancer Hela cells.Methods Cervix cancer Hela cells were cultured in vitro and then different concentration of EB-PAOA were added to Hela cells.The growing curve was drawn.MTT assay was adopted to determine the inhibition rate to Hela cells.Results EB-PAOA can inhibit the proliferation of Hela cells,and the inhibition rate is of time and dose dependent.Conclusions EB-PAOA has obvious inhibition effect on Hela cells.
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Objective To explore the effects of iNOS inhibitor 1400 W alone or combined with cisplatin to cervical cancer cell line HeLa. Methods Cervical cancer cell line HeLa was resuscitated and incubated,MTT was used to investigate 1400 W and cisplatin’s effects on HeLa cell growth; flow cytometry was used to detect the HeLa cell apoptosis rate and RT-PCR was used to detecte the E6, p53 mRNA expression of HeLa cell. Results Both cisplatin and 1400 W showed a dose-dependent inhibitory effect on HeLa cell proliferation(P0.05). 1400 W could not increase HeLa cell’s apoptosis, while 1400 W combined cisplatin could both induce apoptosis and decrease E6, p53 mRNA expression of HeLa cell(P0.05). Conclusions 1400 W combined with ciplatin can increase the HeLa cell apoptosis rate than the group cisplatin used alone.Combined with iNOS inhibitor, it can enhance the cisplatin effect on tumor cell. This is possible a promising adjuvant therapy for cervical caner.
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フッ素による培養細胞の増殖抑制機構を検討するため, HeLa細胞ならびにフッ素耐性HeLa細胞 (FR細胞) を用いて実験を行った。耐性細胞は最高2mMのNaFを添加した培地でHeLa細胞を継代し樹立したものである。その結果次の成績が得られた。1. HeLa細胞の増殖は, 0.5mMNaFでわずかに抑制され, 2mMNaFでは全く阻止された。FR細胞は, 2mM NaFでも対照と同じ増殖を示した。2. 2mMNaF存在下でHeLa細胞のタンパク質合成は強く阻害されたが, FR細胞でのタンパク質合成阻害はわずかであった。3. HeLa細胞とFR細胞の細胞質のタンパク質組成をSDS-ポリアクリルアミドゲル電気泳動 (SDS-PAGE) で調べたところ, クーマシーブルー染色で, 両細胞間のペプチドバンドのパターンに明らかな違いは認められなかった。4. HeLa細胞とFR細胞を, 2mM NaF処理した後合成されたタンパク質をSDS-PAGE, オートラジオグラフィーで調べたところ, HeLa細胞では, 分子量約77000ダルトンの強くラベルされる特異的なペプチドが認められた。FR細胞では, NaFの作用をうけないHeLa細胞と同様なペプチドバンドのパターンが見られた。5. この特異的な77000ダルトンのペプチドは, フッ素処理時間の増加と共に強くラベルされる傾向を示した。6. 77000ダルトンのペプチドの細胞内局在は, 形質膜, ミトコンドリア分画, ミクロゾーム分画に認められたが, 核, 可溶性分画には認められなかった。
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Bacteriostatic activity of Acridine Orange against E. coli was inhibited by the addition of anionic surfactants. The inhibitory activity of surfactants decreased with a decrease in the carbon number of alkyl chain of the surfactants. The apparent concentration of Acridine Orange to inhibit the growth of E. coli was increased by the addition of surfactants. It was concluded that the complex formation between Acridine Orange and surfactants resulted in a decrease of free Acridine Orange in the culture media.
Acridine orange
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Acridine derivatives
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Objective To observe the effects of different concentration of TRAIL on cervical cancer HeLa cells.Methods With different concentration of TRAIL role in HeLa cells,the survival fraction of HeLa cells was measured by MTT assay in Chongqing Medical University in 2006.Structures of apoptosis cells were observed by AO/EB fluorescence microscopy.Cell cycle of HeLa cells and apoptosis rates were determined by FACS.Results After treating HeLa cells for 24 h by TRAIL,a good dose-effect relationship was demonstrated by the role of TRAIL in the rate of cell growth inhibition and apoptosis;early and late apoptosis cell can be seen by AO/EB stainning and the hypodiploid peaks were found by FACS checking.The cell cycles were changed.Conclusion The proliferation of HeLa cells can be inhibited and the apoptosis can be induced by TRAIL.
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