Cells of origin of the spinocerebellar tract in the rat, studied with the method of retrograde transport of horseradish peroxidase

The mechanism of the uptake of horseradish peroxidase (HRP) by damaged post-ganglionic sympathetic axons was studied in vitro. HRP was applied to the damaged axons at the time of nerve injury or after a 3 hours or 17 hours delay. An interval of 3 hours or 17 hours between nerve injury and exposure to HRP had no effect on the localisation of the HRP in the damaged axons or on its retrograde transport to their perikarya. Evidence was found for the pinocytotic uptake of the enzyme by the damaged axons and its accumulation within those axons in elongated cisternae and larger rounded vesicles. In further experiments the damaged axons were treated with HRP at 0 degrees C and then washed in HRP-free medium. The tracer entered the axons in a diffuse form under these conditions but no pinocytotic uptake was observed. However, following 24 hours further incubation at 37 degrees C, HRP could not be found in the perikarya. Treatment at 0 degrees C did not produce any lasting damage to the retrograde transport mechanism. The results of these experiments are compatible with the involvement of pinocytosis in the uptake of HRP in a form suitable for retrograde transport.

Horseradish peroxidase

Axoplasmic transport

Pinocytosis

Free nerve ending

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Citations (3)

Demonstration of nigrothalamic connections in the cat by retrograde axonal transport of horseradish peroxidase

Brain Research (1975)

Eric Rinvik

Horseradish peroxidase

Axoplasmic transport

10.1016/0006-8993(75)90312-1

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Citations (123)

Demonstration of nigro-thalamic connections in the cat by retrograde axonal transport of horseradish peroxidase

Brain Research (1975)

Eric Rinvik

Horseradish peroxidase

Axoplasmic transport

10.1016/0006-8993(75)90113-4

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Citations (0)

Identification of different size motoneurons labeled by the retrograde axonal transport of horseradish peroxidase

Pflügers Archiv - European Journal of Physiology (1977)

Monica Pellegrini O. Pompeiano N. Corvaja

Horseradish peroxidase

Axoplasmic transport

10.1007/bf01063471

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Citations (9)

[Afferent connections to the thalamic centromedian nucleus in the cat as studied by the retrograde axonal transport techniques of horseradish peroxidase (HRP)].

PubMed (1984)

J. Kawahira

Horseradish peroxidase

Axoplasmic transport

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[Demonstration of the neurosecretory hypothalamo-rhombencephalic junction in rats by the retrograde axonal transport of horseradish peroxidase].

PubMed (1978)

F Schober

The retrograde transport of horseradish peroxidase (HRP) was used to demonstrate the neurosecretory hypothalamo-hindbrain connection of the rat. Following HRP injections into the region of the dorsal columns nuclei labeled cells were observed in the caudal part of the paraventricular nucleus and in the lateral hypothalmic area. Hypothalamo-hindbrain projections are predominantly uncrossed.

Horseradish peroxidase

Axoplasmic transport

Hindbrain

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Citations (3)

The uptake and retrograde transport of horseradish peroxidase-polylysine conjugate by ligated postganglionic sympathetic nervesin vitro

Journal of Neurocytology (1981)

Patrick N. Anderson A.R. Medlen J. Mitchell D. Mayor

Horseradish peroxidase

Axoplasmic transport

Conjugate