Automated amino acid determination by high-performance liquid chromatography with 2-(9-anthryl)ethyl chloroformate as precolumn reagent
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Chloroformate
Ethyl chloroformate
Carbamate
Ethyl chloroformate
Chloroformate
Carbazole
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FMOC-Cl is a derivatizing agent widely used both in N-protection reactions of a-amino acids as well as in quantification and detection of amino acids and related molecules using HPLC-PDA or fluorescence analysis. In this work we studied the reaction of FMOC-Cl with gabapentin. However, it was observed that the degradation of gabapentin-N-FMOC and FMOC-Cl to FMOC-OH occur simultaneously in presence of the basic reaction medium which is specific for gabapentin derivatization. The degradation reaction is accelerated in temperatures above 45 °C and also by the time of contact of the reactant and product with basic medium. In this study, gabapentin derivatization reactions were shown to be cleaner and more efficient when an FMOC-Cl/gabapentin ratio of 2:1 was used, at 25 °C over a period of 15 minutes.
Chloroformate
Ethyl chloroformate
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A new method for the separation of amino acids by capillary electrophores is used and 9_(2_carbazole)ethyl chloroformate(CEOC) as derivatization is developed. The effect of some important factors on the separation was studied. Under the selected conditions, good separation was obtained .
Chloroformate
Ethyl chloroformate
Carbazole
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Chloroformate
Ethyl chloroformate
Ethyl ester
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Chloroformate
Ethyl chloroformate
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A simple and rapid method for the separation of amino acids by capillary zone electrophoresis using 9(2carbazole)ethyl chloroformate (CEOC) as derivatization agent was developed. The effect of some key factors such as pH, temperature and concentration of buffer, on the separation of amino acid derivatives was investigated. 12 amino acids were separated with satisfactory results.
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Ethyl chloroformate
Carbazole
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Abstract Six aromatic biogenic amines, three aromatic amino acid precursors, four aromatic hydroxycarboxylic acid catabolytes, and two aromatic glycols were analyzed by capillary GC after derivatization. Instantaneous derivatization was performed by reaction with ethyl chloroformate in a medium of aqueous acetonitrile containing ethanol and pyridine. After extraction of the derivatives into chloroform, the sample was ready for analysis.
Ethyl chloroformate
Chloroformate
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Chloroformate
Ethyl chloroformate
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Micellar electrokinetic chromatography
Chloroformate
Ethyl chloroformate
Sodium dodecyl sulfate
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A method for the analysis of the optical purity of a series of chiral substituted tetrahydroisoquinolines (THIQs) was developed. The method is based on pre-column derivatization of the analytes with the derivatization reagent (–)-(1R)-menthyl chloroformate. The derivatization reaction selectively gives diastereomeric carbamates that are resolvable on an achiral non-polar GC column. The developed technique covers variously substituted THIQs, which differ significantly in volatility, steric and electronic properties. In all cases, the resolution factors (R) exceeded the value of 1.5. The method represents a robust way of analysis of mixtures of THIQs, which are often present in various matrixes such as body fluids, tissues and reaction mixtures.
Chloroformate
Diastereomer
Ethyl chloroformate
Ethylamine
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