Selection of new biological activities from random nucleotide sequences: evolutionary and practical considerations

Genome (1989)

Marshall S. Horwitz Dipak K. Dube Lawrence A. Loeb

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Abstract:

Recent advances in the selection of biologically active DNA sequences from random populations are reviewed. Within the framework of evolution, forces are considered that have precluded the testing of all possible DNA sequences, purely with regard to their functionality as genetic regulatory elements or protein coding sequences. Examples are drawn from cassette mutagenesis of enzyme active sites, protein domain replacement by fusion with random genomic digests, and the selection of bacterial promoters from random DNA. Efforts to derive new activities are examined, and the likelihood of future success is evaluated.Key words: active DNA, nucleotide permutation, DNA evolution.

Keywords:

Molecular evolution

Topics:

RNA and protein synthesis mechanisms

Advanced biosensing and bioanalysis techniques

Bacteriophages and microbial interactions

10.1139/g89-021

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Blackberry extracts inhibit UV-induced mutagenesis in Salmonella typhimurium TA100

Nutrition Research (2006)

Patricia L. Tate Anne Stanner Kelly Shields Samuel Smith Lyndon L. Larcom

10.1016/j.nutres.2005.11.005

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Citations (17)

[ENU induced mutagenesis-large-scale study of gene mutagenesis and function].

PubMed (2001)

Yi Sun Xiaoying Yang

ENU mutagenesis is becoming one of the most powerful approaches for large-scale study of gene function. The mechanism of ENU mutagenesis, factors that affect mutation rates, the strategies of the ENU mutagenesis, screening for the phenotypes, related researches and recent progresses were introduced.

Site-directed mutagenesis

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Mutagenesis in Plant Breeding

George Acquaah

This chapter contains sections titled: Purpose and expected outcomes Brief historical perspectives Types of mutations Mutagenic agents Types of tissues used for mutagenesis Factors affecting the success of mutagenesis Mutation breeding of seed-bearing plants Mutation breeding of clonally propagated species Mutations from tissue culture systems Using induced mutants Limitations of mutagenesis as a plant breeding technique Selected significant successes of mutation breeding Molecular techniques for enhancing efficiency of induced mutagenesis Horticultural applications of mutagenesis General effects of mutagenesis Key successes of induced mutagenesis Outcomes assessment

Mutation Breeding

Site-directed mutagenesis

Directed mutagenesis

Forward genetics

10.1002/9781118313718.ch23

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Citations (0)

Mutagenesis as viewed from another perspective.

PubMed (1990)

Michael M. Lieber

Mutation has generally been considered a random process, not directed. Thus, a mechanism allowing for adaptively responsive mutagenesis has conceptually been precluded. However, an adaptively directed mutagenesis in response to environmental stress can be based upon genetic mutator systems. Such systems, known for years, have been seen as exceptional and not considered in terms of an adaptively responsive mutagenesis. Environmental stress which invokes this type of mutagenesis can thus be regarded as mutagenic itself, and our concept of what is mutagenic must therefore be broadened. Relevant matters are also discussed.

Directed mutagenesis

Site-directed mutagenesis

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Citations (3)

Altered CTP synthetase activity confers resistance to 5-bromodeoxyuridine toxicity and mutagenesis

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis (1986)

Elliot R. Kaufman

Chinese hamster

Insertional mutagenesis

Bromodeoxyuridine

Thymidine