Studies on a Temperature-sensitive Irreparable Mutant of <i>Neurospora Crassa</i>

The Japanese Journal of Genetics (1960)

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Abstract:

(1) Three temperature-sensitive mutants were obtained from a microconidial wild type strain of Neurospora crassa. One of them is irreparable at 34°C; that is, it grows as well as the wild type at 23°C, but does not grow on any (including omplete) medium so far tested at 34°C. This mutant was the major subject of this report.(2) The temperature-sensitive irreparable mutant b39 differs from the parent strain by a single gene mutation. The gene responsible for the irreparable character is located on the first linkage group (mating type chromosome), about 30cM left of the centromere.(3) This mutant does not inhibit the growth of the other temperature-sensitive strains in the heterocaryotic condition.(4) The possibility of the accumulation of inhibitory substances by the mutant is unlikely, because the culture filtrate of the mutant did not inhibit the growth of the mutant at an intermediate temperature.(5) The growth characteristics of the mutant when the culture was transfered from 23°C to 34°C, and from 34°C to 23°C were determined.(6) The nature of the defect in an irreparable mutant was discussed.

Keywords:

Neurospora

Wild type

Crassa

Strain (injury)

Topics:

Fungal and yeast genetics research

Microbial Metabolic Engineering and Bioproduction

Photosynthetic Processes and Mechanisms

10.1266/jjg.35.49

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PHASE-SPECIFIC GENES FOR MACROCONIDIATION IN NEUROSPORA CRASSA

Genetics (1974)

Claude P. Selitrennikoff Robert E. Nelson Richard W. Siegel

ABSTRACT Two new mutant genes in Neurospora crassa prevent the formation of free macroconidia from proconidial chains. These genes, called conidial separation-1 and conidial separation-2, are phase-specific, playing no role in either the sexual life cycle or other aspects of the asexual life cycle. A cell-wall-associated autolytic activity was found to increase in wild-type cultures at the time of active formation of free conidia from proconidial chains; no such increase was detected in mutant cultures. It appears that the products of these genes are both essential for and unique to macroconidiation.

Crassa

Neurospora

10.1093/genetics/78.2.679

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Citations (65)

[4] Genetic and microbiological research techniques for Neurospora crassa

Methods in enzymology on CD-ROM/Methods in enzymology (1970)

Rowland H. Davis Frederick J. de Serres

Neurospora

Crassa

Filamentous fungus

Germ tube