Investigation of hippocampal volume and trauma symptoms in older and young adults
Shireen SindiAlexandra FioccoRobert‐Paul JusterIsabelle Ouellet‐MorinMarie France MarinCatherine LordJens C. PruessnerSonia Lupien
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Objective:To explore the method of culturing rat hippocampal slice in vitro and to observe the changes of neurons' morphology and reactivity in cultured slice.Methods:Organotypic hippocampal slices were prepared by taking brains from 6-9 day old wistar rats.After the hippocampi was isolated and chopped into 400μm thickness,slices were transferred to Millipore inserts and placed in six-well trays in CO_2 incubators at 37℃.Then the cultured hippocampal slices were observed for the changes of morphology by macroscopic observation and using inverted phase contrast microscope;Expression of Fos protein in cultured hippocampal slice was detected by immunocytochemistry for observation of the internal response of hippocampal neurons to the damage;The electrophysiological activity of hippocampal neurons were detected by using patch clamp technique. Results:(1)The number of neurons in cultured hippocampal slices was increased,and the thickness of cultured hippocampal slices was decreased markedly along with the culturing period in vitro.After being cultured for 4 weeks,the thickness of hippocampal slices was decreased to about 150μm.(2)With the treatment of pilocarpine to induce seizure-like activity,the number of neurons which expressing Fos protein was increased in CA1 area of the cultured hippocampal slice.(3)With the application of pathch clamp technique,the changes of the ion currents of pyramidal neurons in CA1 area were recorded by the whole-cell recording after culturing for 1 week,2 weeks,3 weeks and 4 weeks respectively.Conclusion:Cultured hippocampal slice in vitro could retain satisfactory liveness and function for at least 4 weeks.
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Objective To investigate the effect of acanthopanacis senticosi polysaccharides(ASPS)on apoptosis of rat hippocampal neurons.Methods The hippocampal neurons were isolated from SD rats aged less than 3 days and cultured,then induced to apoptosis by H2O2.After treated with ASPS,the changes of cell form were observed,the viability of hippocampal neurons was detected with MTT method and the expressions of Bax and Bcl-2 were detected by immunohistochemical method.ResultsThe cell injury of hippocampal neurons in ASPS-treated group was slighter than that in model group.The viability of hippocampal neurons in ASPS-treated group(0.46)was higher than that in model group(0.36)(P0.01).The hippocampal neurons in ASPS-treated group had higher expression of Bcl-2 and lower expression of Bax.ConclusionASPS enhances the anti-apoptotic capacity of hippocampal neurons in a concentration-dependent manner.
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Objective:To evaluate the effect of Polychlorinated Biphenyls(PCB) in cultured hippocampal neuronic cells of rats.Methods:After rats' hippocampal cells were cultured with different PCB, MTT was used to observe the growth and development of hippocampal cells.Results:It was obvious that hippocampal neuronic cells was inhibited in the highest groups of PCB,10 -4 group was apparently lower than that of control group(P0.01);10 -7 and 10 -6 groups were lower than that of control group(P0.05);The live rate of cell was reduced.Conclusion:PCB had strong nerve toxicity to hippocampal neuronic cells.
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Objective To observe the protective effect of brain-derived neurotrophic factor(BDNF) on Aβ25-35-induced primarily cultured hippocampal neuron injury.Methods Hippocampal cells taken from new-born Wistar rats were incubated in vitro,into which different concentrations of BDNF and aggregated Aβ25-35 were added to act on the cultured hippocampal neurons.Effect of BDNF on the survival rate of hippocampal neurons and apoptosis of hippocampal cells induced by Aβ25-35 was observed by MTT and Hoechst 33342 staining,respectively.Results The damage of hippocampal cells was less severe in BDNF treatment group than in Aβ25-35 model group,and the hippocampal cells grew well under microscope.MTT showed that the survival rate of hippocampal cells increased with the increased concentration of BDNF.Hoechst 33342 staining showed that the apoptosis rate of hippocampal cells decreased with the increased concentration of BDNF,which was lower in BDNF treatment group than in Aβ25-35 model group(P0.01).Conclusion BDNF can protect the cultured hippocampal neurons against injury induced by Aβ25-35 due to its neurotrophic activity.
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Objective:To investigate the protective effects of naoyikang on D-gal-induced injury in cultured hippocampal neurons in neonatal SD rat.Methods: By way of seropharmacology to collect the sera containing Naoyikang.Cultured hippocampal neurons were treated with D-gal.The protective effect of naoyikang were evaluated by cell morphology and colorimetric MTT assay.Results:Naoyikang can significantly increased the number of hippocampal neurons.Conclusion:Naoyikang can protect hippocampal neurons injuried from D-gal-induced injury.
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