Peroxisome proliferator-activated receptors (PPARs) in skin health, repair and disease
185
Citation
70
Reference
10
Related Paper
Citation Trend
Keywords:
Human skin
Sebaceous gland
To establish an in vitro culture model of single human hair follicle, and observe their morphological and histological changes.Human hair follicles were isolated from the volunteer patients. After dissecting follicles into single, follicles in growth phase were cultured in Williams E without any serum. This experiment included 3 groups: single follicle without sebaceous gland and other surrounding tissue (control group); single follicle with sebaceous gland and without the other surrounding tissue( experiment group A); single follicles with sebaceous gland and the other surrounding tissue (experiment group B). The survival rate, survival time, growth rate, multiplication capacity and apoptosis of cultured follicles and their morphological and histological changes were observed sequentially.The hair follicles in experiment groups showed a better viability and a higher growth rate than those in control group. And the follicles in group B could keep growing for more than 25 days, which was longer than those in group A. Moreover, the sebaceous gland and the other surrounding tissue in group B showed great induction effect on follicle-cell proliferation and anti-apoptosis.The in vitro culture model of signal human hair follicles (single follicles including epidermis, sebaceous gland and the other surrounding tissue) had optimized internal environment which is similar to in vivo internal environment.
Sebaceous gland
Epidermis (zoology)
Cite
Citations (1)
Abstract Terence Kealey first pioneered the isolation and organ maintenance of human eccrine and sebaceous glands in the early to mid‐1980. This led to subsequent methods describing the isolation and culture of human hair follicles, the human pilosebaceous unit as well as the sebaceous duct. The importance of these models in the study of the biology of human skin glands and appendages has been demonstrated in numerous publications and their importance as models for animal replacement, refinement and reduction (3Rs) is increasingly important. In particular, in vitro (ex vivo) hair follicle culture has played a significant part in helping elucidate the role of signalling molecules in regulating hair growth and hair fibre formation and has been especially useful in understanding metabolic aspects of hair growth. However, obtaining sufficient numbers of hair follicles is becoming increasingly difficult as plastic surgery becomes less invasive and smaller skin samples provided. There is therefore an urgent requirement for the next generation of in vitro models using cell lines and tissue engineering, and this has led to the development of immortalised cell lines as well as attempts to model hair follicle embryogenesis in vitro and development of skin on a chip.
Organ culture
Sebaceous gland
Human skin
Ex vivo
Dermal papillae
Cite
Citations (32)
Skin samples of nine body areas from each of four adult and two young onehumped camels were collected and standard histological techniques were followed to study the histomorphology of the skin. The epidermis of the camel skin possessed four layers characteristics of the epidermis in the skin of mammals. The dermis was divided indistinctly into superficial and deep reticular layers and the dermis was composed of collagen elastic and reticular fibers; hair follicles, sebaceous and sweat glands and arrector pili muscles. Hair follicles distributed singly and evenly in the upper lip while they were grouped in distinct clusters in other body areas studied. The tactile hairs were present in the upper lip. Multilobulated sebaceous glands were associated with each tactile hair follicle. Sebaceous glands were simple, branched alveolar gland and were associated with hair follicles. Each group of secondary hair follicles had its own sebaceous gland unit. No glands were observed in the skin areas without hair follicles. Sweat glands were present in all areas in which they were associated with the large primary hairs and none were seen with small secondary hairs or in the upper lip.
Sebaceous gland
Reticular connective tissue
Epidermis (zoology)
Dermal papillae
Vellus hair
Cite
Citations (1)
Generation of skin appendages in engineered skin substitutes has been limited by lack of trichogenic potency in cultured postnatal cells. To investigate the feasibility and the limitation of hair regeneration, engineered skin substitutes were prepared with chimeric populations of cultured human keratinocytes from neonatal foreskins and cultured murine dermal papilla cells from adult GFP transgenic mice and grafted orthotopically to full-thickness wounds on athymic mice. Non-cultured dissociated neonatal murine-only skin cells, or cultured human-only skin keratinocytes and fibroblasts without dermal papilla cells served as positive and negative controls respectively. In this study, neonatal murine-only skin substitutes formed external hairs and sebaceous glands, chimeric skin substitutes formed pigmented hairs without sebaceous glands, and human-only skin substitutes formed no follicles or glands. Although chimeric hair cannot erupt readily, removal of upper skin layer exposed keratinized hair shafts at the skin surface. Development of incomplete pilosebaceous units in chimeric hair corresponded with upregulation of hair-related genes, LEF1 and WNT10B, and downregulation of a marker of sebaceous glands, Steroyl-CoA desaturase. Transepidermal water loss was normal in all conditions. This study demonstrated that while sebaceous glands may be involved in hair eruption, they are not required for hair development in engineered skin substitutes.
Dermal papillae
Sebaceous gland
Human skin
Artificial skin
Cite
Citations (46)
Sebaceous gland
Epidermis (zoology)
Infundibulum
Inner root sheath
Cite
Citations (3)
Sebaceous gland
Inner root sheath
Cite
Citations (0)
Hair follicle(HF) is a tissue to produce hair,which continuously cycles through periods of intensive growth(Anagen),apoptosis-driven regression(Catagen),and resting(Telogen).The HF and sebaceous gland constitute the pilosebaceous unit of the skin.This paper described the factors for regulation of HF periodicity,sebaceous gland development as well as hair lustrousness and hair felting at molecular level.These can provide significant thoughts in enhancing the production and quality for cashmere.
Sebaceous gland
Cite
Citations (0)
The epithelial cells and dermal cells of hair follicle are much similar to the keratinocytes and dermal fibroblast of skin in phenotype respectively, but it has great proliferation ability and differentiation potential, as well as biological property. And the dermal cells of hair follicle share some property of stem cells. So there is a great particular advantage as seed cells for skin tissue engineering in which it could constitute artificial skin with affiliated organs such as hair follicle and sebaceous gland.
Sebaceous gland
Dermal fibroblast
Artificial skin
Cite
Citations (0)
Cite
Citations (16)
Sebaceous gland
Hair shaft
Cite
Citations (0)