Effect of treatment on oligoclonal IgG bands and intrathecal IgG synthesis in sequential cerebrospinal fluid and serum from patients with subacute sclerosing panencephalitis
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Subacute sclerosing panencephalitis
Immunofixation
Intrathecal
Immunofixation
Haptoglobin
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Intrathecal
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In the cerebrospinal fluid of 26 children with SSPE the levels of prealbumins and total proteins were determined and electrophoretic separation of proteins was done. The usefulness is discussed of the percent proportion of prealbumins in the cerebrospinal fluid as a criterion indicating damage to the blood-cerebrospinal fluid barrier with increased penetration of proteins in SSPE. It is concluded that in diseases in which biosynthesis occurs of proteins in the central nervous system the percent proportion of prealbumins fails to reflect the integrity of the brain-cerebrospinal fluid barrier.
Subacute sclerosing panencephalitis
Cerebrospinal fluid proteins
Penetration (warfare)
CSF albumin
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Immunofixation
Polyacrylamide
Thin layers
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This paper describes the detection of oligoclonal bands of IgG immunoglobulins in cerebrospinal fluids (CSF) using isoelectric focusing (IEF) and immunofixation with the PhastSystem equipment. The proteins were separated by IEF on polyacrylamide gels, pH 3-9. The IgG immunoglobulins were then fixed by overlaying the gel with antihuman IgG antiserum. Fixed immunoglobulins were detected with silver staining in the PhastSystem Development Unit. One microliter of serum or CSF containing 15 mg/L of IgG was found to be optimal. The procedure had good reproducibility both with regard to IEF and silver staining of the fixed immunoglobulins. The manpower need was reduced, and the procedure has been found to be useful in the clinical laboratory.
Immunofixation
Silver stain
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Isoelectric focusing of monoclonal antibodies in agarose gel is one of several ways to characterize a hybridoma. Isoelectric focusing is performed on each monoclonal antibody, and the mouse immunoglobulin bands are immobilized by soaking the gel with rabbit antimouse immunoglobulin before staining with Coomassie Blue.
Immunofixation
Agarose
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Subacute sclerosing panencephalitis
Immunofixation
Kappa
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Immunofixation
Kappa
Polyclonal antibodies
Immunoperoxidase
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Immunofixation
Protease inhibitor (pharmacology)
Alpha (finance)
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ORM phenotypes were studied by hybrid isoelectric focusing (HIEF) followed by immunofixation and silver staining. The method was applied to the study of ORM in human serum and minute bloodstains. Given the promising results observed, the use of HIEF for forensic purposes is recommended.
Immunofixation
Orosomucoid
Silver stain
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