Chromosomal behavior of anther culture derived plants of rice
14
Citation
11
Reference
10
Related Paper
Citation Trend
Keywords:
Polyploid
Callus
Plant biochemistry
Doubled haploidy
The purpose was to find the optimum conditions and formula of inducing loquat anther callus.With loquat anther as tested material,the influences of temperature,sucrose concentration and hormone on inducing loquat anther callus were studied by complete combination design.Low temperature treatment made little influences on callus formation.Compared with CK,the callus induction was lower along with low-temperature time prolonging.The effect of inducing anther callus with 30 g/L sucrose was better,with the induction rate of 62.5%.The induction rate of MS medium added with 1 mg/L 2,4-D and 2 mg/L 6-BA was highest,being 90.00%.The medium of MS+0.25 mg/L6-BA +1 mg/LNAA was most favorable to callus proliferation.The sucrose with high concentration was not favorable to callus formation.2,4-D and 6-BA with certain concentration had greater influences on loquat anther callus and 2,4-D played leading role.
Callus
Cite
Citations (0)
Agarose-solidified media containing maltose and glutamine were used to produce double-haploid lines, by anther culture, from Australian spring barley (Hordeum vulgare L.) genotypes. Three sets of F1 and two sets of F2 breeder's hybrids were used. Genotype differences were significant for the proportion of anthers responding, and the number of green and albino regenerants per 100 anthers plated. It was confirmed that the environmental conditions experienced by the donor plants (time of year, and field or glasshouse) had a large impact upon explant performance in culture. A minimum level of anther culture efficiency was achieved to allow a breeding program to produce all its inbred lines by this method. The procedure has the potential to save research costs, and deliver improved genotypes to the grower up to four years earlier than conventional methods.
Doubled haploidy
Somaclonal variation
Plant Breeding
Cite
Citations (19)
Doubled haploidy
Plantlet
Plant Breeding
Cite
Citations (0)
The Objectives of rice breeding program in Indonesia is to obtain high yielding varieties, tolerant, resistant to abiotic and biotic stress and appropriate to planting system in specific area. To accelerate the obtainment of the varieties, a combination of conventional and no conventional breeding method can be used. One alternative procedure is anther culture. Doubled haploid lines can be obtained through cholchicine treatment or rattooning of haploid plants. Plants with high heterozygositas (F1 or F2) can be used as anther source to obtain genetic variability of doubled haploid plants. High degree of homozigousity can be obtained at the first (DHO) generation of doubled haploid plants, less than on year. Evaluation of agronomic characters follows m DHI and DH2 generations. Compared to conventional methods, the use of anther culture in rice breeding program has several advantages including efficiency of selection process, reduction of cost, time and labor. Effort to optimize anther culture method and its rize in rice breeding program in Indonesia is discussed. Key words: Anther culture, Rice
Doubled haploidy
Breeding program
Rice plant
Cite
Citations (9)
Doubled haploids can improve the efficiency of breeding and genetic study in apple ( Malus × domestica Borkh.). Seventeen homozygous genotypes were obtained by in vitro anther culture from ‘Senshu’ apple. Flow cytometry analysis revealed that the ploidy level of the anther-derived plantlets was diploid. Simple sequence repeat (SSR) analysis determined the origin and homozygous status of the anther-derived plantlets. The results of S-RNase polymerase chain reaction (PCR)-digestion analysis reinforced the homozygous state. The morphological characteristics and reproductive potential of the doubled haploids were investigated. It is especially interesting that one of the doubled haploids derived from anther culture had fertile pollen grains (rate of in vitro germination was 20.6%–33.0%), and several progenies were obtained from the cross between ‘Prima’ apple and the doubled haploid. The hybridism of these seedlings was confirmed by SSR analysis. Furthermore, the female gametes of the doubled haploid showed slight fertility. Thus, this doubled haploid will be valuable for breeding and genetic studies.
Doubled haploidy
Malus
Polyploid
Cite
Citations (9)
[Objective]The present experiment was aimed to study the effects of different hormone combinations on callus induction in anthers taken from tomato thermo-sensitive genic male sterile lines(TSGMSLs) and their hybrid combinations.[Method]Tomato TSGMSLs,viz.,Da 107 and Di D4,and their hybrid combinations,viz.,Da 107×Niufanqie and Di D4×Hongkunlun were used in the studies.Combinations of different hormones(CPPU,TDZ,KT) in basic MS medium(containing 1.0 mg/L 2,4-D+1.0 mg/L 6-BA) were used for anther callus induction.[Result]Adding different CPPU,TDZ or KT concentrations in basic MS medium significantly improved the induction of anther callus,and the suitable concentration of CPPU,TDZ and KT was 0.4-0.6,0.03 and 1.5 mg/L,respectively.Under the same hormone combination conditions,the anthers taken from tomato TSGMSLs showed better callus induction compared to those taken from hybrid combinations,the order of the average rate of callus induction and average diameter of callus was:Di D4Da 107Di D4×HongkunlunDa 107×Niufanqie.[Conclusion]Anthers of different tomato genotypes differed in their sensitivities to different hormones.Furthermore,different combinations of hormones also differed in affecting induction of tomato anther callus,out of them,the combinations of KT and 2,4-D+6-BA showed best results.
Callus
Cite
Citations (0)
Effects of hormone,florescence,corolla state,light and pH value on inducing callus of Patriot blueberry anther in vitro were studied.The results showed that:WPM+BA 0.5 mg/L+2,4-D 1.0 mg/L medium was most suitable for anther callus induction.Corolla with 2:1 of length and width during full flowering stage was the best period for sample collection.Culture under darkness condition promoted the callus induction of blueberry anther,with an induction rate of 56.67%.There were no obvious differences in callus induction rate when medium pH value varied from 4.98 to 5.50.
Callus
Darkness
Cite
Citations (0)
In order to further develop and utilize the resource of Rhodiola sachalinensis,taking its callus induction anther as materials,the effect of different medium,different type and concentration of plant hormone and cytokin on anther callus growth were studied.The results showed that on anther callus growth had no significant difference,the lower concentration of IAA could promote the grouth of anther callus,and the effect of 2,4-D was higher than that of NAA.The higher concentration of 6-BA could promote the qrowth of anther callus.With the same basal medium,6-BA was more suitable for the growth of anther callus than KT.
Callus
Cite
Citations (0)
Doubled haploidy
Common wheat
Cite
Citations (37)
The aim of this study was to compare the efficiency of broccoli anther and microspore culture methods for doubled haploid (DH) lines production. We evaluated the main influencing factors and optimized the culture methods to improve embryo induction and plant regeneration for efficient doubled haploid production in broccoli breeding. Six broccoli hybrids were used in this study. Our results show that generally, the efficiency of androgenic embryogenesis and regeneration in microspore culture is higher than that in the anther culture. Moreover, the microspore culture eliminated the possibility of plantlets coming from diploid tissue. In this study, the four-day cold pre-treatment yielded the highest number of embryos in both anther and microspore culture methods; the embryo yield at 32.5°C for 24 h was the highest in anther and microspore culture. Optimal plating densities were 30 anthers per dish in anther culture and 4×105 microspores per ml in microspore culture. In androgenic embryo production, the PG-96 medium proved to be more effective than NLN medium. Sucrose concentration at 10% for anther culture and 13% (w/v) for microspore culture was recommended. A total of 70 regenerants were obtained from three genotypes including doubled haploids, haploids and aneuploids. Key words: Anther, broccoli, doubled haploid, microspore, plant regeneration.
Doubled haploidy
Microspora
Cite
Citations (6)