Orally active desulfated low molecular weight heparin and deoxycholic acid conjugate, 6ODS-LHbD, suppresses neovascularization and bone destruction in arthritis
Seung Rim HwangDong-Hyun SeoTaslim A. Al-HilalOk-Cheol JeonJin Hee KangSung-Hyun KimHan Sung KimYoung‐Tae ChangYoung Mo KangVictor C. YangYoungro Byun
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Umbilical vein endothelial cells are known to be able to produce interleukins and colony stimulating factors. In the present work supernatant of human umbilical vein endothelial cell culture have been administered to neoplastic patients treated with chemotherapy to reduce the iatrogenic inhibition of hemopoiesis. While no undesired effect could be observed, neutrophil count was favourably influenced by endothelial cell supernatant administration. Such data can be considered useful in order to reduce collateral effect of antineoplastic therapy.
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AIM: To investigate the injuring effect of DMSO-soluble particles from cigarette smoke(DSP) on human umbilical vein endothelial cells. METHODS: Human umbilical vein endothelial cell line EA. hy 926 was used as target cells in the study. The growth and viability of the cells treated with various dosages (1, 2, 4 or 4 mL/L) of DSP and low dose (2 mL/L) of DSP at different time points were evaluated by MTT colorimetric assay and celllular protein assay in 96-well plates. Transmission electron microscopy study was carried out to observe the ultrastructure of human umbilical vein endothelial cells under DSP treatment.RESULTS: DSP inhibited the proliferation of human umbilical vein endothelial cell line EA. hy 926. Under DSP treatment, the reducing cellular protein and increasing cell death(mainly necrosis) were observed in time-dependent and dosage-dependent manners.CONCLUSIONS: These results indicated that the toxic effect of DSP caused functional disturbance and structural damage of human endothelial cells.
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AIM:To observe the effects of porphyromonas gingivalis(Pg) to the proliferation and apoptosis of human umbilical vein endothelial cells(HUVECs) in vitro,so as to evaluate the virulence of Pg to HUVECs.METHODS:An aeropack was used to culture Pg,and HUVECs were derived from primary culture of human umbilical vein.The MTT was employed to assay the proliferation of HUVECs.The apoptosis of HUVECs was detected by in situ cell apoptosis detection kit I.RESULTS:Pg obviously inhibited the proliferation of HUVECs compared with lipopolysaccharide,and apoptosis can also be observed in HUVECs after treated by Pg.CONCLUSION:The Pg might injure the human umbilical vein endothelial cells(HUVECs) and to aggravate the inflammatory reaction in periodontitis,which could be one of the pathologic mechanisms in cardiovascular disease.
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Objective:To abserve the effect of rapamycin on the proliferation of human umbilical vein endothelial celI(HUVEC)in vitro.Methods:HUVEC were exposed to different concentrations of rapamycin (0.1ng/ml-20ng/ml) solution,and the influence of rapamycin on cell proliferation induced by VEGF was investigated by MTT assaying method.Results:Various concentrations rapamycin inhibited cell proliferation.Conclusion:rapamycin had the concentration dependent inhibitory effect in HUVEC.The mechanism of rapamycin on neovascularization is to inhibit the proliferation of HUVEC.
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Objective To investigate the effects of FAP on human umbilical vein endthelial cell proliferation and angiogenesis in vitro.Methods Human umbilical vein endothelial cell EA.hy926 was treated with different concentrations of FAP(0,300,600,1200 pmol/L).Cell proliferation was determined by MTT assay.Cell migration was assessed by a wound healing assay.And tube formation was studied with a three-dimensional gel model.Results Compared with the normal control group,FAP promoted endothelial cell migration and proliferation(P0.05),promoted tube formation of endothelial cell on the gel(P0.01).The effect of 600 pmol/L FAP was significantly higher than that of other groups(P0.01).Conclusion FAP can promote human umbilical vein endothelial cell proliferation,migration and angiogenesis in vitro.It provides a basic theoretical depiction of the role of FAP in angiogenesis.
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Purpose To explore the role of green tea on basic fibroblast growth factor (bFGF) in human breast cancer cells and human umbilical vein endothelial cells (HUVECs) and its mechanisms. Methods bFGF concentrations were determined by ELISA. Northern blot hybridization was performed to detect the expression of mRNA. Results 40 μg/mL GTE or EGCG could decrease the levels of bFGF peptide secreted into conditioned media as well as the bFGF peptide levels in both HUVECs and human breast cancer cells; this effect was dose dependent. 40 μg/mL GTE and EGCG decreased the mRNA levels of bFGF in MDA-MB231 cells. Conclusions Green tea can inhibit bFGF expression in human breast cancer cells and human umbilical vein endothelial cells through multiple levels. This may be the partial mechanism for green tea to inhibit angiogenesis in cancers.
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ABSTRACT Therapeutic angiogenesis is a promising approach to treat ischemic skin flaps. We delivered basic fibroblast growth factor (bFGF) to the recipient bed of a rat dorsal skin flap by a drug delivery system with acidic gelatin hydrogel microspheres (AGHMs), and assessed augmentation of neovascularization and flap viability. An axial skin flap was elevated on the back of male Sprague–Dawley rats, and bFGF solution or bFGF‐impregnated AGHMs were injected into the recipient bed. The dose of bFGF in the bFGF solution was set to 15 (Sol‐15 group), 50 (Sol‐50 group), or 150 μg (Sol‐150 group). Correspondingly, 2 mg AGHMs were impregnated with 15 (AGHM‐15 group), 50 (AGHM‐50 group), or 150 μg (AGHM‐150 group) bFGF. Other groups of animals received phosphate‐buffered saline (Sol‐Cont group) or phosphate‐buffered saline‐impregnated AGHMs (AGHM‐Cont group) as controls. Seven days later, analyses of the area of necrosis, microangiographic findings, and histological findings in the flap were carried out. The area of necrosis in the AGHM‐150 group was significantly smaller than that in the other groups. Microangiographic and histological analyses showed that neovascularization of the ischemic skin flap significantly increased in the AGHM‐150 group as compared with the Sol‐150 group and the AGHM‐Cont group. These findings suggest that continuous delivery of bFGF to the recipient bed by bFGF‐impregnated AGHMs enhances the viability of an ischemic skin flap.
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Objective: To explore the molecular mechanism of the proliferation of human umbilical vein endothelial cells induced by AngⅡ.Methods: The lines of human umbilical vein endothelial cell cultured in vitro were divided into 3 groups which were treated by AngⅡ, AngⅡ+NAC, and normal culture medium respectively. First we observed the proliferous effect of human umbilical vein endothelial cells induced by AngⅡ at different concentration at different time with biochemical methods. Then the contents of ROS(·OH) in 3 groups were detected by spectrophotometer. Finally we detected the related gene expression in the process of human umbilical vein endothelial cells proliferation via the technique of genechips.Results: Human umbilical vein endothelial cells incubated with AngⅡ(0.03125～1μmol/L ) for 12 hours increased the proliferation rate (P0.05);the negative correlation between proliferation rate and the contents of ROS(·OH)(r=-0.8, P0.01) was significant;NAC can reduce the content of ROS(·OH) and inhibit the proliferation of human umbilical vein endothelial cells;The technique of genechips analysis suggests that the genes expression related to proliferation such as ERK, Akt, CCN and PCNA increased, while the gene expression related to apoptosis such as DR6, Caspases6 BAK1 and PDCD8 decreased when human umbilical vein endothelial cell were induced by AngⅡ(0.0625μmol/L) for 12 hours; In contrast, when human umbilical vein endothelial cells were induced by AngⅡ(1μmol/L) for 12 hours, we can obtain reverse results. Conclusion: Human umbilical vein endothelial cells induced by AngⅡcan produce ROS(·OH).The target gene expression related to proliferation of human umbilical vein endothelial cells may be mediated by ROS(·OH), ROS(·OH) may be the molecules which play an important role in the signal transduction and gene expression of proliferation.
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Objective To investigate the proangiogenic effects of total flavone of Abelmoschus manihot(L.) Medic.(TFA) in human umbilical vein endothelial cells(HUVEC).Methods The HUVEC cultured in vitro served as the model to determine the cell proliferation with MTT.The effect of TFA on HUVEC migration was detected by Transwell chamber migration assay,and cell differentiation was examined by endothelial tube formation assay.Results Treatment with 5-20 μg/ml TFA for 72 h had a marked effect on the proliferation of HUVECs,and strongly promoted the migration,10-20 μg/ml TFA could promote tube formation of HUVEC.Conclusion TFA at certain concentrations exerts a potent proangiogenic effect on HUVEC in vitro.
Manihot
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AIM: To investigate the effect of basic fibroblast growth factor (bFGF) on C-type natriuretic peptide (CNP) production, release and mRNA expression. METHODS: Human endothelial cell cultured; CNP was measured by radioimmunoassay method;CNP mRNA expression was determined by RT-PCR technique. RESULTS: bFGF could augment CNP synthesis in human endothelial cells. Compared with control group,25 ng, 50 ng, 100 ng bFGF increased CNP contents in endothelial cells by 88% (P0.05), 95% (P0.05), 187% (P0.01), respectively.100 ng bFGF also stimulated CNP release from cultured human endothelial cell. In addition, 25 ng, 50 ng and 100 ng bFGF stimulated CNP mRNA expression of cultured human endothelial cells in a dose-dependent manner. CONCLUSION: bFGF might regulate CNP synthesis,release and mRNA expression in cultured umbilical human endothelial cells.
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