Genomic constitution of a partial amphiploid OK7211542 used as a source of immunity to barley yellow dwarf virus for bread wheat
33
Citation
26
Reference
10
Related Paper
Citation Trend
Abstract:
Abstract A combination of genomic in situ hybridization (GISH) and meiotic pairing analysis of crosses between a series of 2 n = 56 partial amphiploids confirmed that the alien genome of the BYDV‐immune Agro‐tricum line OK7211542 is derived from Thinopyrum ponticum and not from Thinopyrum intermedium. The evidence from meiotic pairing analysis indicated that the chromosome constitution of OK7211542 is similar to another Agrotricum line, ORRPX, which was derived from a cross of wheat and Th. ponticum , but different from other Agrotricum lines, Zhong 5 and TAF 46 which were derived from the crosses between wheat and Th. intermedium. The GISH analysis confirmed that OK7211542 contained one complete set of 14 Th. ponticum chromosomes, in which no S chromosome was present in the alien genome. GISH also detected a small alien translocation attached to one of the wheat chromosomes, a result that was consistent with the pairing data.Keywords:
Barley yellow dwarf
Common wheat
Molecular cytogenetics
The foundation and development of molecular cytogenetics are closely related to cytogenetics and molecular genetics. It is microcosmic as well as macroscopic development in depth in studying cytogenetics and molecular genetics. The wide applications of molecular cytogenetics in animal heritable breeding are mostly displayed in gene locating and gene mapping, molecular markers and markers assistant selection, heterosis forecast and origin, classification and evolution of species.
Molecular cytogenetics
Molecular Genetics
Molecular marker
Cite
Citations (0)
Molecular cytogenetics
Molecular Genetics
Cite
Citations (5)
Cytogenetics is the study of chromosomes; their structure and properties, chromosome behavior during cell division, their influence on traits and factors which cause changes in chromosomes. Veterinary cytogenetics is the application of cytogenetics to clinical problems that occur in animal production. It has been applied to understand problems such as infertility and its types, embryonic and fetal death, abnormality in sexual and somatic development and hybrid sterility and also prenatal sex determination and other forms of chromosomal abnormalities. These are achieved through conventional and banded karyotyping techniques and molecular cytogenetic techniques. Although conventional techniques are still useful and very widely applied, the nature of cytogenetics has gradually changed as a result of advances achieved in the molecular cytogenetic techniques for example fluorescent in situ hybridization and array-based techniques. These changes are evident in both molecular diagnostics and basic research. The combination of conventional and molecular cytogenetics has given rise to high resolution techniques which have enabled the study of fundamental questions regarding biological processes. It enables the study of inherited syndromes, the mechanisms of tumorigenesis at molecular level, genome organization and the determination of chromosome homologies between species. It allows the ease with which animals are selected in breeding programs and other important aspects of animal production. In this paper we discussed a number of techniques employed in cytogenetics and their methodologies, and recommend where future focus should be for the benefits of animal production.
Molecular cytogenetics
Cite
Citations (2)
The aim of this article is not to present an exhaustive review of molecular cytogenetics applications in domestic animal species, but more to illustrate the considerable contribution of these approaches in diagnostics and research in economically important species. A short presentation of the main applications of molecular cytogenetics in humans points out the domains in which it has become an essential tool and underlines the specificities attached to this species in comparison to farm animals. This article is devoted to outlining the current resources available in domestic species and to some examples of fluorescence in situ hybridization applications in the cattle, pig, horse and avian species. From a clinical point of view, these examples illustrate the advantages of FISH for the study of chromosomal abnormalities (identification, characterization and estimation of their effects). Other applications of molecular cytogenetics are also illustrated, particularly ZOO-FISH, an approach which allows the determination of chromosome homologies between species. Finally, a specific emphasis was placed on the usefulness of molecular cytogenetics for the analysis of species such as poultry, which harbour a complex karyotype.
Molecular cytogenetics
Cite
Citations (32)
Cytogenetics and molecular cytogenetics are and will continue to be indispensable tools in cancer diagnostics. Leukemia and lymphoma diagnostics are still emphases of routine (molecular) cytogenetics and corresponding studies of solid tumors gain more and more prominence. Here, first a historical perspective of molecular tumor cytogenetics is provided, which is followed by the basic principles of the fluorescence in situ hybridization (FISH) approach. Finally the current state of molecular cytogenetics in cancer diagnostics is discussed. Nowadays routine diagnostics includes basic FISH approaches rather than multicolor-FISH. The latter together with modern high-throughput methods have their impact on research to identify new tumor-associated genomic regions.
Molecular cytogenetics
Molecular diagnostics
Molecular Genetics
Cite
Citations (21)
Cytogenetics is the study of chromosomes. The involved techniques have an indispensable role in chromosome aberration detection. Taking the invention of in situ hybridization (ISH) as a dividing line, all techniques are categorized into classical and molecular cytogenetics. Typical techniques in classical cytogenetics are chromosome banding and flow karyotyping. Branches of ISH, such as fluorescence in situ hybridization, spectral karyotyping, and comparative genomic hybridization, are often classified as molecular cytogenetics. All of these techniques have provided researchers with either quantitative or qualitative data to aid in the analysis of chromosomes and have made significant contributions to the fields of prenatal detection, prognostic, diagnostics, oncology, etc.
Molecular cytogenetics
Comparative genomic hybridization
Cite
Citations (1)
The effect of molecular cytogenetics in pathogenesis of multiple myeloma (MM) is fundamental.The risk stratification system that based on molecular cytogenetics of MM is widely applicable in the prognosis of MM.The aberration of molecular cytogenetics is the most important marker of MM.At the same time,high-risk MM is associated with intra-clonal tumor heterogeneity and clonal evolution.The research of intra-clonal tumor heterogeneity and clonal evolution which based on the aberration of molecular cytogenetics will provide some additional information of the biology of MM and contribute to the optimum treatment for MM patients.
Key words:
Multiple myeloma; Molecular cytogenetics; ASH annual meeting
Molecular cytogenetics
Pathogenesis
Molecular Genetics
Cite
Citations (0)
Fluorescence in situ hybridization (FISH) assays are indispensable in diagnostics and research. Routine application of this so-called molecular cytogenetic technique on human chromosomes started in 1986. Since then, a huge variety of different approaches for chromosomal differentiation based on FISH has been described. It was established to characterize marker chromosomes identified in conventional banding analysis as well as cryptic rearrangements not resolved by standard cytogenetics.Even though molecular cytogenetics, like banding cytogenetics for almost 40 years, is often called dead now, it offers unique possibilities of single cell analysis. Thus, a review is presented here on the available diagnostic-relevant FISH methods and probe sets applied in routine pre- and postnatal clinical as well as tumor cytogenetics.Molecular cytogenetics is a fast, straightforward and reliable tool that is indispensable in cytogenetic diagnostics. It is and will continue to be of high clinical impact in diagnostics, especially in the overwhelming majority of routine cytogenetic laboratories that cannot afford and do not need high-throughput chip-based platforms for their daily work.
Cite
Citations (2)
The concept of molecular cytogenetics, its history, and perspectives are introduced here. FISH applications in clinical and tumor genetic diagnostics, including diagnostic guidelines and quality control, are reviewed. The impact of molecular cytogenetics in nowadays' research is discussed, and finally a unique collection of internet pages is provided dealing with cytogenetics, molecular cytogenetics, as well as closely related fields.
Molecular cytogenetics
Cite
Citations (2)
Abstract In the pre‐genomics era, the field of comparative cytogenetics was restricted to the comparison of banded chromosome preparations of the species being studied. The development of molecular biology and subsequent convergence of this field with conventional cytogenetics led to the development of molecular cytogenetics. The application of molecular cytogenetics, in particular multicolour fluorescence in situ hybridization (MFISH), has revolutionized our ability to compare genomes at the chromosomal, subchromosomal and molecular levels, leading to much more defined knowledge about the processes of chromosomal rearrangement that have occurred during evolution. In this article we review very briefly some of the work that has led to a greater understanding of the comparative cytogenetics of mammals, in the context of their relationship to the human karyotype.
Molecular cytogenetics
Comparative genomic hybridization
Cite
Citations (23)