Is the lack of concurrence of bacterial vaginosis and vaginal candidosis explained by the presence of bacterial amines?
30
Citation
14
Reference
10
Related Paper
Citation Trend
Keywords:
Germ tube
Candida glabrata
Vaginal flora
Candida krusei
Non-albicans Candida (NAC) species are emerging as important opportunistic pathogens. This has significant clinical impact as, NAC species have decreased susceptibility to commonly used antifungal agents. Hence species identification in the Clinical Microbiology laboratory is essential. This study was conducted to assess efficacy of HiCrome agar to reliably identify Candida to the species level. Altogether 48 isolates of Candida were isolated during a period of one year constituting 24 isolates of Candida albicans, 13 of Candida tropicalis, 4 of Candida krusei, 4 of Candida glabrata, 2 of Candida parapsilosis and 1 isolate of Candida guillermondii. Identification was done based on microscopic morphology, germ tube test, growth at 45°C, morphology on corn meal agar and colony colour on HiCrome agar. HiCrome agar accurately identified all species of Candida albicans, Candida tropicalis, Candida krusei and Candida glabrata. Two isolates of Candida parapsilosis and one isolate of Candida guillermondii was misidentified as Candida glabrata. HiCrome agar can be used as a fairly reliable and time saving alternative to conventional methods with good sensitivity and specificity.
Candida krusei
Candida parapsilosis
Candida glabrata
Germ tube
Cite
Citations (11)
To examine variability in bacterial vaginosis (BV) over 6- to 12-month intervals.One thousand one hundred ninety-three women were followed for a median of 3 years with serial vaginal swab Gram stains for BV. Discrete time hazard models were fit to identify independent risk factors for BV.Women with BV at study entry were categorized as having normal flora at the next visit 20% of the time, and women with normal flora at study entry were categorized as having BV at the next visit 20% of the time. Among women with initially normal flora, factors associated with BV were black race, lower education, a history of BV, a history of chlamydial/gonococcal cervicitis, and lack of monogamy.About one fifth of women with normal flora develop BV over a given 6- to 12-month interval, and the modifiable risk factors of cervicitis and lack of monogamy contribute to the development of BV.
Vaginal flora
Cervicitis
Chlamydial infection
Flora
Cite
Citations (38)
Vaginal flora
Flora
Vaginal infections
Leukorrhea
Vaginal disease
Trichomonas Vaginitis
Cite
Citations (0)
Candida is normal flora of human skin, mouth, vagina, colon and causes mycosis worldwide. Candida causes oral thrush, vaginitis and other potentially life-threatening diseases. This study assesses anticandidal potential of essential oils against Candida species isolated from clinical samples obtained from Mahatma Gandhi Institute of Medical Sciences, Sewagram, Wardha, Maharashtra. On the basis of morphological characterization on Sabouraud dextrose agar, CHROMagar, Germ tube test and biochemical characterization, Candida species identified as Candida albicans, Candida krusei, Candida glabrata, Candida parapsilosis. Clove, thyme, cinnamon and eucalyptus essential oils with concentrations of 6.2, 12.5, 25, 50 and 100 μg/ml were used to study their anticandidal activity. C. albicans demonstrated zone of inhibition (ZOI) 29, 20, 32, 29 mm, Candida krusei 25, 25, 30, 21 mm, Candida glabrata 24, 22, 31, 32mm,Candida parapsilosis 33,31,33, 29mm towards clove, thyme, cinnamon and eucalyptus oil (each at 100 μg/ml) respectively which are highest ZOI as compared with antifungal agent Amphotericin-B, which showed 16, 13, 12, 8 mm against C. albicans, C. krusei, C.glabrata and C. parapsilosis respectively. All Candida spp. produced biofilms and enzyme lipase. This study will facilitate the development of novel broad-spectrum key molecules against a range of Candida species.
Candida krusei
Candida parapsilosis
Candida glabrata
Germ tube
Cite
Citations (0)
Vaginal flora
Flora
Gardnerella vaginalis
Sialidase
Cite
Citations (16)
Candida krusei
Candida glabrata
Melissa Officinalis
Cite
Citations (0)
ObjectivesInfection with Candida glabrata and Candida krusei represents a major challenge. We sought to describe outcomes for patients with candidaemia/invasive candidiasis (C/IC) due to these pathogens who were treated with micafungin.
Candida krusei
Candida glabrata
Invasive candidiasis
Candida infections
Cite
Citations (21)
Objective: To study the clinical distribution and drug resistance of fungus in our hospital and to provide a scientific theory to use anti-fungus. Methods: Fungus were identified by chomargar medium and ATB Fungus 3 were used for susceptibility test. Results: There were 1 217 elderly candida, Candida albicans were 598 strains(49.14%), non Candida albicans 619 strains(50.8614%); in non Candida albicans, the 3 most were Candida glabrata, Candida tropicalis, Candida krusei, and the proportion were 21.69%(264 strains), 19.64%(239 strains), 4.68%(57 strains)respectively. The position where isolated were the most was respiratory tract 77.98%(949 cases), then urinary tract 10.44%(127 cases), stool 9.61%(117 cases). The susceptibility test show Candida albicans, Candida glabrata, Candida tropicalis and Candida parapsilosis had high susceptibility rate to 5FC, AMB, VRC, But the susceptibility of Candida.krusei to 5FC, AMB were 38.90%, 89.70% respectively. To FCA, ITR, Candida glabrata, Candida krusei have some resistant rate and SDD. Conclusions: Nowadays the fungus in clinic were mostly candida, and Candida albicans has the highest isolation rate, and non Candida albicans were more than before, the maior were Candida krusei, Candida tropicali, Candida glabrata, at the same time the resistant rate to anti-fungus were higher than before.
Candida krusei
Candida glabrata
Candida parapsilosis
Cite
Citations (0)
Bacterial vaginosis (BV) is an extremely common health problem for women. In addition to the troublesome symptoms often associated with a disruption in the balance of vaginal flora, BV is associated with adverse gynecological and pregnancy outcomes. Although not technically a sexually transmitted infection, BV is a sexually associated condition. Diagnostic tests include real-time clinical/microbiological diagnosis, and the current gold standard, the standardized evaluation of morphotypes on Gram stain analysis. The inappropriate use of vaginal culture can be misleading. Future developments into molecular-based diagnostics will be important to further understand this complex endogenous flora disruption.
Vaginal flora
Gram staining
Flora
Gold standard (test)
Vaginal secretion
Vaginal discharge
Cite
Citations (61)
The interactions between pathogens during infection and the impact of these interactions on drug effectiveness are poorly understood, making polymicrobial infections challenging to treat. During an infection, cross-interactions between bacteria and fungi can strengthen virulence mechanisms and affect how the disease develops. The purpose of this study is to determine how Pseudomonas aeruginosa interacts with Candida glabrata, Candida albicans, Candida krusei, Candida parapsilosis, and Candida tropicalis in the development of polymicrobial biofilms. Pseudomonas aeruginosa, Candida albicans, Candida krusei, Candida parapsilosis, Candida glabrata, and Candida tropicalis isolates were used in this experimental investigation. After preparing a 0.5 Mc Farland suspension of each isolate, the gold standard for measuring biofilm was applied: the Tissue Plate Culture (TCP) method. After that, an ELISA reader with a wavelength of 595 nm was used to measure the optical density (OD) of the biofilm. SPSS 26.0 was then used for statistical analysis to compare the OD values between Pseudomonas aeruginosa that had not been exposed to Candida and those that had. Pseudomonas aeruginosa and Candida are found to interact synergistically if there is an increase in OD, and antagonistic interaction is discovered if there is a decrease in OD. In comparison to the group that was not exposed to Candida, Pseudomonas aeruginosa exposed to Candida albicans, Candida krusei, Candida parapsilosis, Candida glabrata, and Candida tropicalis showed an increase in the OD value of biofilm. Pseudomonas aeruginosa and Candida albicans, Candida glabrata, Candida krusei, Candida parapsilosis, and Candida tropicalis interact synergistically.
Candida krusei
Candida parapsilosis
Candida glabrata
Candida dubliniensis
Cite
Citations (0)