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    A plasmid rescue technique for the recovery of plant DNA disrupted by T-DNA insertion
    Plant Molecular Biology Reporter (1992)
    Friedrich J. BehringerJune I. Medford
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    Keywords:
    In vitro recombination
    T-DNA Binary system
    Transfer DNA
    Cloning vector
    Topics:
    Plant tissue culture and regeneration
    Plant Genetic and Mutation Studies
    Chromosomal and Genetic Variations
    Plasmid incompatibility: cloning analysis of an inc FII determinant of R6-5
    Nature (1978)
    Kenneth N. TimmisIsabel AndrésPatrick M. Slocombe
    PstI
    Cloning vector
    T-DNA Binary system
    Autonomously replicating sequence
    Restriction map
    Citations (59)
    Plasmid pBRINT: a vector for chromosomal insertion of cloned DNA
    Gene (1993)
    Paulina BalbásXóchitl AlvaradoFrancisco BolívarFernando Valle
    Cloning vector
    In vitro recombination
    Multiple cloning site
    Cloning (programming)
    Citations (12)
    EXPERIMENTAL TECHNIQUES AND STRATEGIES FOR DNA CLONING
    Elsevier eBooks (1979)
    Stanley N. Cohen
    In vitro recombination
    Extrachromosomal DNA
    Replicon
    Cloning vector
    Cloning (programming)
    T-DNA Binary system
    Multiple cloning site
    Plasmid preparation
    Citations (0)
    Structure and expression of DNA transferred to tobacco via transformation of protoplasts with Ti-plasmid DNA: co-transfer of T-DNA and non T-DNA sequences
    Plant Molecular Biology (1985)
    Frans A. KrensRuud M.W. MansTruus M. S. van SlogterenJ. Harry C. HogeG. J. Wullems
    Transfer DNA
    T-DNA Binary system
    In vitro recombination
    Ti plasmid
    Library
    Protoplast
    Citations (35)
    Site-specific insertion of genes into T-DNA of the Agrobacterium tumor-inducing plasmid: an approach to genetic engineering of higher plant cells.
    PubMed (1981)
    Matzke AjChilton
    This paper presents a method for insertion of genetic material into a specific site in T-DNA, the portion of Agrobacterium tumor-inducing (Ti) plasmid that becomes incorporated into the nuclear DNA of transformed plant cells when crown gall tumors are incited by this plant pathogen. The three stages of our procedure are as follows: 1. A T-DNA subfragment cloned in pBR322 is cleaved by a restriction endonuclease at a unique central site and target DNA (a kanamycin resistance marker) is ligated into this site. 2. The resulting recombinant plasmid is purified and cleaved with EcoRI; the resulting fragment bearing the kanamycin resistance marker is ligated into the unique EcoRI site of pRK290, a wide-host-range plasmid. 3. The pRK290 recombinant plasmid is transformed into an agrobacterium tumefaciens strain containing a wild type Ti plasmid. Double recombination between the altered T-DNA fragment of the clone and its wild-type counterpart in the Ti plasmid is selected for by introduction of R751-pMG2, a plasmid incompatible with pRK290. The approach described here can be adapted for introducing genes into higher plant cells with the Ti plasmid as vector. It can likewise be used for site- or fragment-specific mutagenesis of the Ti plasmid as a means of detailed functional analysis.
    T-DNA Binary system
    Ti plasmid
    Transfer DNA
    In vitro recombination
    Citations (85)
    Direct cloning of cDNA inserts from λgt11 phage DNA into a plasmid vector by a novel and simple method
    Gene Analysis Techniques (1990)
    Ing‐Ming ChiuKirsten Lehtoma
    Cloning vector
    Insert (composites)
    Subcloning
    In vitro recombination
    Phagemid
    Multiple cloning site
    Cosmid
    Cloning (programming)
    Citations (5)
    A modified pBR322 vector with improved properties for the cloning,recovery, and sequencing of blunt-ended DNA fragments
    Gene (1982)
    Pierre PrentkiHenry Krisch
    Cloning vector
    In vitro recombination
    Cloning (programming)
    Multiple cloning site
    Citations (67)
    Cloning of Herpes simplex virus type 2 DNA fragments in a plasmid vector
    Gene (1980)
    Denise A. GallowayM A Swain
    Cloning vector
    In vitro recombination
    Restriction map
    Restriction fragment
    Cloning (programming)
    Citations (24)
    The Plasmid, pBR322
    Elsevier eBooks (1988)
    Paulina BalbásXavier SoberónFrancisco BolívarRaymond L. Rodriguez
    Cloning vector
    Extrachromosomal DNA
    Cloning (programming)
    In vitro recombination
    Multiple cloning site
    Autonomously replicating sequence
    Citations (26)
    Highly Efficient Yeast-Based in Vivo DNA Cloning of Multiple DNA Fragments and the Simultaneous Construction of Yeast/ Escherichia Coli Shuttle Vectors
    BioTechniques (2006)
    Ei’ichi IizasaYukio Nagano
    In vivo recombinational cloning in yeast is a very efficient method. Until now, this method has been limited to experiments with yeast vectors because most animal, insect, and bacterial vectors lack yeast replication origins. We developed a new system to apply yeast-based in vivo cloning to vectors lacking yeast replication origins. Many cloning vectors are derived from the plasmid pBR322 and have a similar backbone that contains the ampicillin resistance gene and pBR322-derived replication origin for Escherichia coli. We constructed a helper plasmid pSU0 that allows the in vivo conversion of a pBR322-derived vector to a yeast/E. coli shuttle vector through the use of this backbone sequence. The DNA fragment to be cloned is PCR-amplified with the addition of 40 bp of homology to a pBR322-derived vector. Cotransformation of linearized pSU0, the pBR322-derived vector, and a PCR-amplified DNA fragment, results in the conversion of the pBR322-derived vector into a yeast/E. coli shuttle vector carrying the DNA fragment of interest. Furthermore, this method is applicable to multifragment cloning, which is useful for the creation of fusion genes. Our method provides an alternative to traditional cloning methods.
    Shuttle vector
    Multiple cloning site
    Cloning vector
    In vitro recombination
    Cloning (programming)
    Autonomously replicating sequence
    Citations (31)