Function of the transplanted canine heart after prolonged preservation by simple immersion
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Washout
Viaspan
瞄准：在人的阴茎海绵体在试管内上评估 verapamil 的弛缓的效果并且为可勃起的机能障碍(编辑) 作为一个处理估计这药的潜力。方法：人的阴茎海绵体的准备从最近被获得有正常的死亡的年轻人可勃起的功能。当 10 micromol/L 脱羟肾上腺素导致的收缩被 verapamil 或车辆控制(无菌的水) 的不同剂量减少时，等轴的紧张和详细曲线被记录。人的阴茎海绵体准备的紧张在增加 verapamil 或车辆前被描述为他们的最高的紧张的一个百分比。ANOVA 和最少的有效差量测试被用于统计分析。结果：1 micromol/L， 10 micromol/L 和 100 micromol/L verapamil 的剂量导致了松驰(35.28+/-7.96 )% ，(55.91+/-6.41 )% ，(85.68+/-4.16 ) 在 30 min 以后的 % 分别地。车辆控制同时削尖生产松驰(-0.06+/-10.57)%(P<0.05 ) 。结论：Verapamil 在放松脱羟肾上腺素在试管内导致的正常人的语料库多孔的平滑肌是显著地有效的，弛缓的效果取决于 verapamil 的集中。
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Initial flush for cooling and coronary vascular washout using University of Wisconsin (UW) solution has presented some problems. We used a separate solution for initial flush and for subsequent storage, and evaluated the effect of initial flush for prolonged heart preservation. Following potassium arrest, the initial flush was performed using UW solution (UW group) or Physiol · 3® solution with additional potassium (PK group). In the PK group, an additional flush with the UW solution was done prior to preservation. The hearts of both groups were stored in the cold UW solution for 12 hours. Myocardial high-energy phosphates were measured using phosphorus 31 nuclear magnetic resonance spectroscopy (UW group: n = 7, PK group: n = 5). Adenosine triphosphate (ATP) levels in the UW group decreased to 23% of the control after storage. ATP levels in the PK group were well maintained for up to 6 hours, and then decreased to 51% (p < 0.01). Orthotopic transplantation was performed in 10 pairs of dogs (UW group: n = 7, PK group: n = 3). Preserved grafts in the PK group (74%) tended to show better recovery of left ventricular pressure than those in the UW group (52%). These results suggest that the introduction of an initial flush may prevent ATP level reduction during cold storage, and may provide good results after transplantation. An initial flush for cooling and coronary vascular washout is important for prolonged heart preservation.
Viaspan
Washout
Adenine nucleotide
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Drug overdose
Calcium channel blocker
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当教室で樹立した人培養腎癌細胞株 (NUR) および人正常腎細胞 (PHK) を用い, ビンクリスチン (VCR), アドリアマイシン (ADM), シスプラチン (CDDP) に対する verapamil の効果増強作用について検討した. 殺細胞効果については, 細胞の増殖曲線, 3H-thymidine incorporation 法で検討した. また細胞内における3H-VCR, 3H-daunomycin の取り込みを経時的に測定し, 取り込みおよび放出に対する verapamil の影響をみた. verapamil の濃度は細胞毒性を示さない1.0μg/mlとした. verapamil は NURcell の増殖曲線, 3H-thymidine incorporation のいずれにおいてもADM, VCR, CDDPの殺細胞効果を増強させ, 3H-thymidine incorporation の抑制でみるとIC50 (drug concentration for 50% inhibition) は各々制癌剤単独で0.095, 4.81, 1.01μg/mlであったものが, verapamil 1.0μg/mlの併用で, 0.039, 1.29, 0.508μg/mlとなった. またNUR cell に対し, ADMの効果増強を生じる verapamil の最小濃度は, 約0.1μg/mlであり, その増強効果は濃度依存性に高められた. 3H-VCR, 3H-daunomycin のNUR cell への取り込みは verapamil によって, いずれも増加した. しかし細胞外放出に対しては, 3H-VCRは verapamil によって抑制される傾向を示したが, 3H-daunomycin については, その影響を認めなかった. PHK cell についても 3H-thymidine incorporation によってVCR, ADM, CDDPに対する verapamil の影響を検討したが, いずれも軽度であった. また, PHK cell への3H-VCR, 3H-daunomycin の取り込みに対する verapamil の影響も極軽度であった.
Thymidine
IC50
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The antihypertensive effects of immediate-release (IR) verapamil were compared with those of sustained-release (SR) verapamil in 58 patients. After an open-label (IR verapamil) study, patients were randomized into a double-blind study to continue receiving the same dose of IR verapamil three times daily or an equivalent dose of SR verapamil (240 to 480 mg) once daily. Blood pressure decreased from 149/98 to 139/90 mmHg (p less than 0.01) with IR verapamil and from 150/98 to 136/88 mmHg (p less than 0.01) with SR verapamil. Ambulatory blood pressure monitoring showed a similar response for the two formulations. Diastolic pressure was less than 90 mmHg in approximately 67% of the IR verapamil group and 61% of the SR verapamil group. Mean trough plasma concentrations of verapamil were 70 and 59 ng/ml at 2 and 4 weeks, respectively, after treatment with IR verapamil; the corresponding values were 70 and 94 ng/ml for the SR verapamil group. SR verapamil administered once daily is an effective antihypertensive medication in a selected group of patients and could afford better compliance.
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The aim of this work was to determine the influence of ethanol on the antiarrhythmic activity of verapamil in the model of calcium arrhythmia in rats non-dependent and dependent on ethanol. The results of the experiment show that a combined, single administration of ethanol and verapamil attenuates in a statistically significant manner the antiarrhythmic effect of verapamil. Ethanol administered repeatedly together with verapamil does not diminish the antiarrhythmic activity of verapamil.
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Objective:To observe the influence of VP-16 on Tca8113,the cells of carcinoma of tongue,and the enhancement effect of verapamil in the environment of the laboratory,.Methods: The VP-16's effect on Tca8113 cells by MTT was studied and attempt was made to combine verapamil and VP-16 on Tca8113 cells to evaluate the influence of verapamil on VP-16.Results: VP-16 could markedly inhibit the growth of Tca8113.Verapamil in itself could not inhibit the growth of Tca8113 cells.5mg/l verapamil performed for 1h in advance could increase the effect of VP-16.Conclusion: VP-16 can markedly inhibit the growth of Tca8113 cells,and verapamil can obviously intensify the VP-16 effect.
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Stereospecificity
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Fifty-two cases of hypertension were treated with verapamil. The obvious effective rate was 86.5% and the total effective rate 98.1%. The antihypertensive effect of verapamil was confirmed, as compared with nifedipine. A preliminary approach of the dosage of verapamil showed that a total dose of 120-240 mg daily could achieve satisfactory result clinically in most patients.
Therapeutic effect
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