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    Abstract:
    We aimed to assess the distribution of human papillomavirus (HPV) genotypes in high-grade cervical lesions in Taiwan. The study included 1,086 paraffin-embedded, formaldehyde-fixed cervical intraepithelial neoplasia (CIN) 2/3 specimens. HPV genotyping was performed using polymerase chain reaction (PCR)-based methods. Multiple HPV types were validated by E6 type-specific PCR, direct sequencing and/or real-time PCR. HPV DNA was detected in 995 (91.6%) specimens, and multiple HPV types were identified in 192 (19.3%) samples. The leading HPV types were HPV16 (24%), HPV52 (20%), HPV58 (20%), HPV33 (13%), HPV31 (8%) and HPV18 (4.6%). Although the leading six types consisted of 87.6%, HPV16 or 18 comprised only 30.9%. The prevalence of different HPV types showed a significant association with age. In women older than 50 yr, HPV16 and 18 comprised 21.3% (83/389), while HPV52, 58 and 33 represented 55.5% (216/389). In women aged less than 50 yr, HPV16 and 18 comprised 32.1% (224/697, p < 0.0001), while HPV 52, 58 and 33 represented 47.9% (334/697, p = 0.02). The distribution of HPV genotypes was compared with previously reported findings for Taiwanese women with cervical cancer (CC). The overall HPV16 positivity rate was significantly higher in CC than in CIN 2/3 (odds ratio: 2.14, 95% CI: 1.91-2.40). In addition, HPV18, 39 and 45 were significantly overrepresented in CC, whereas HPV52, 58, 33, 31, 35, 51 and 53 were underrepresented. We concluded that an effective vaccine against the most common HPV types could prevent a significant proportion of cervical cancer cases that occur in Taiwan.
    Knowledge of the risk factors for cervical cancer (CC) and the benefits of cervical cancer prevention motivates women to participate in preventive screening. However, several studies indicate that thereis a significant lack of knowledge about the human papillomavirus (HPV) around the world. This study examines the level of knowledge about the prevention of HPV and cervical cancer in the contextof socio-demographic and behavioral characteristics of women in Uzbekistan.Key words: questioning, cervical cancer, Human Papillomavirus, prevention
    Cancer Prevention
    Objective To analyze the expression of eIF 4A in cervical cancer and different levels of cervical neoplasia tissues and its clinical significance. Methods 80 cases of cervical cancer were included in the diagnosis of cervical cancer, 40 patients with high cervical intraepithelial neoplasia (CIN grade II and grade III) and 40 cases of low grade cervical intraepithelial neoplasia (CIN I) were incorporated into the experimental group between February 2012 and March 2015 in hospital, and patients with total hysterectomy, pathological diagnosis of normal cervix were included in the control group. Expression level of eIF-4A protein in cervical cancer tissues, different levels of cervical neoplasia tissues and normal tissue was determined by immunohistochemical method, to investigate the relationship between the pathology and clinical grading and staging of cervical cancer. Results The expression of eIF-4A proteins in the different tissues were normal cervical tissue (0.0%), CIN I (52.5%), CIN II and III (77.5%), cervical cancer(81.8%). The positive expression rate was gradually increasing trend, the difference was statistically significant (χ2=77.874, P 0.05). The expression of eIF-4A proteins in cervical cancer were independent of the type of organization(χ2=0.779, P=0.377); related to its histological grad(χ2=8.042, P=0.018); and related to its clinical staging(χ2=6.765, P=0.009). Conclusion The expression of eIF-4A proteins may be associated with the occurrence and development of cervical cancer, it may be plays a role in the cervical cancer initiation process. Key words: Cervix neoplasms; Intraepithelial neoplasia; Immuno ochemistry; eIF-4A
    Grading (engineering)
    Clinical Significance
    Carcinoma in situ
    To explore the dynamic changes of human papillomavirus (HPV) type 16 E5 gene in the development of cervical cancer and the significance of E5 mRNA in early screening of cervical cancer.Paraffin specimens of cervical lesions were collected from 49 cases (HPV positive) during September 2015 to December 2017 According to the standard of FIGO, all cervical lesions were diagnosed as: 13 cases of cervicitis, cervical intraepithelial neoplasia disorders (CIN) Ⅰ in 5 cases, CIN Ⅱ in 18 cases, CIN Ⅲ in 5 cases, 8 cases of cervical cancer. Real-time fluorescence quantitative PCR was used to detect the integrity of E5 gene and the mRNA expression levels of E5, E6 and E 7in cervical tissues.All the 49 cases showed positive HPV16 infection. E5 genetic integrity in CINⅠwas higher than that in cervical inflammation, CIN Ⅱand cervical cancer (P < 0.05), which was also higher than that in CIN Ⅲ, but without statistically significance (P>0.05). The mRNA levels of E5, E6, E7 were the highest in CIN Ⅲ. Compared with E6 and E7, E5 presented superior expression in all types of cervical lesions (P < 0.05), while E 6and E7 mRNA expressions only increased in CIN Ⅲ and cervical cancer.In the patients with HPV16 infection, the integrity of E5 gene in cervical tissues may be related to the occurrence and development of cervical diseases. E5 gene is expected to be the target gene for early screening of cervical cancer.
    Cervicitis
    Koilocyte
    Citations (0)
    Human papillomavirus is recognised as the main cause of cervical cancer. Vaccines to protect against specific HPV types have been developed and a national HPV vaccination programme is due to begin in the UK in September 2008. Girls aged 12 to 13 years of age will be offered one of the vaccines, Cervarix, and it is hoped that it will offer them protection against over 70% of cervical cancer.
    HPV vaccines
    Citations (4)
    Objective To investigate the expressions and clinical significance of DCC and Ki67 in cervical intraepithelial neoplasia(CIN) and cervical cancer. Methods Immunohistochemistry(SP method) was used to detect the expressions of DCC and Ki67 in 16 cases of CIN and 49 cases of cervical cancer.Results The positive expression rate of DCC and Ki67 in chronic cervicitis,CIN Ⅰ~Ⅱ,CIN Ⅲ and cervical cancer were 75.0%,50.0%,37.5%,24.2% and 10.0%,43.8%,75. 0%,87.9% respectively. The positive expression rate of DCC was significantly different between cervical cancer,CIN Ⅲ and chronic cervicitis(P0.05). The positive expression rate of DCC was not related with age,clinical stage and pathologic grade of cervical cancer(P0.05).The expressions of Ki67 in CIN Ⅰ~Ⅱ,chronic cervicitis and cervical cancer were positively correlated with clinical stage and pathological grade of cervical cancer (P0.05). Conclusion DCC test is helpful to the early diagnosis of cervical cancer,while the detection of Ki67 is not only contribute to the early diagnosis of cervical cancer,but also to monitor the development of cervical cancer,which also is an important indicator for patients 'prognosis.
    Cervicitis
    Clinical Significance
    Citations (0)
    Infection with human papillomavirus (HPV) is the first necessary cause for cervical cancer development. Although cervical cancer can be prevented and treated if detected early enough, it is the second biggest cause of female cancer mortality worldwide. Early detection of precursor cervical lesions by screening methods based on cytological and/or HPV testing should be encouraged because it offers protection against invasive cervical cancer. This review discusses the essential issues concerning cervical cancer and provides future prospectives.
    Citations (0)
    Objective:To explore the expressions of 14-3-3σ in cervical cancer and cervical intraepithelial neoplasia(CIN).Methods:The mRNA expression of 14-3-3σ was evaluated by means of real-time fluorogenetic quantitative polymerase chain reaction (RFQ-PCR) in cervical tissues of 28 cervicitis,30 CIN1,30 CIN2,30 CIN3 and 16 cervical cancers.Results:RFQ-PCR showed that the average quantity of 14-3-3σ mRNA in cervical cancer tissues was significantly higher than that in cervicitis tissues(P0.05).While the average quantities of 14-3-3σ mRNA in CINs were similar with cervicitis tissues.Conclusion:14-3-3σ is overexpressed in cervical cancer tissues on transcriptional level,but not in CINs tissues.So 14-3-3σ may play an important role in the pathogenesis of cervical cancer.
    Cervicitis
    Pathogenesis
    Citations (0)
    Objective:To explore the expressions of P16INK4A and Ki-67 in cervical intraepithelial neoplasia(CIN) and cervical cancer and their relationships with HPV16/18 infection.Methods:94 paraffin-embedded cervical samples were selected,including 20 samples of chronic cervicitis,13 samples of CIN Ⅰ,17 samples of CIN Ⅱ,20 samples of CIN Ⅲ and 24 samples of cervical cancer,immunohistochemical method was used to detect the expression levels of P16INK4A and Ki-67,and PCR technology was used to detect HPV16/18 DNA.Results:The positive expression rates of P16INK4A in chronic cervicitis group,CIN Ⅰ group,CIN Ⅱ group,CIN Ⅲ group and cervical cancer group were 0.0%,53.8%,76.5%,95.0% and 100.0%,respectively,there was significant difference in positive expression rate of P16INK4A between CIN Ⅲ group,cervical cancer group and the other groups(P0.05);the positive expression rates of Ki-67 in chronic cervicitis group,CIN Ⅰ group,CIN Ⅱ group,CIN Ⅲ group and cervical cancer group were 0.0%,69.2%,88.2%,95.0% and 91.7%,respectively,there was significant difference in positive expression rate of Ki-67 between CIN Ⅲ group,cervical cancer group and chronic cervicitis group;HPV16/18 mainly infected the patients with CIN of high grade and cervical cancer;the expression levels of P16INK4A and Ki-67 were correlated positively with the degree of cervical lesions and HPV16/18 infection.Conclusion:HPV16/18,P16INK4A and Ki-67 are related to the occurrence and development of cervical cancer.
    Ki-67
    Cervicitis
    Citations (0)
    Objective To discuss the expression of p16 protein in cervical cancer,cervical precancerosis and cervical inflammation,explore the biological marker of diagnosis early in cervical cancer and cervical precancerosis.Methods The expression of p16 protein in 23 cases of cervical cancer,63 cases of cervical intraepithelial neoplasia(CIN) and 20 cases cervical inflammation were detected by immunohistochemical staining,and to analyse and compare the results.Results(1)The positive rates of p16 protein in cervical cancer and CINⅠ,CINⅢ and CINⅠ showed significant difference(P0.05);the positive rates of p16 protein in cervical cancer and cervical inflammation,CIN and cervical inflammation showed significant difference too(P0.05);(2)With the development of cervical lesion,the expression intensity and the positive expression of p16 were elevated uncreasingly(P0.001).Conclusion The expression of p16 is related to cervical cancer and the extent and development of CIN lesion,it is suggested that p16 protein expression may be taken as a biomarkers for early diagnosis of cervical cancer and CIN.
    Koilocyte
    Citations (0)