Association of interleukin-1B and interleukin-1RN polymorphisms with gastric cancer in a high-risk population of Costa Rica
79
Citation
38
Reference
10
Related Paper
Citation Trend
Helicobacter pylori(Hp) infection can cause gastritis and peptic ulcer.The genotyping can show the essence of life.Different genotyping is correlated with different diseases and different genotyping correlated with different resistances.This article observed the Hp genotyping.There were four aspects to discuss:The correlation on different genotyping with the disease;different genotyping resistant;the different genotyping mucosal damage intensity;intervention link of traditional Chinese medicine.A brief summary of the nearly seven years of Hp genotyping research provides a new method to discover new drugs for the treatment of Hp with traditional Chinese medicine.
Cite
Citations (0)
Objective:Classified HBV DNA of AsC of children and their parents. Methods:Using a genotyping method of based on the restriction fragment length polymorphism (RFLP) of amplified segment of the HBV S region.Results: Among the AsC of children living in Guangzhou,genotype B is 62.7%,genotype C is 33.3%,genotype B and C is 3.3%,only 1.7% could not be classified.Conclusion:The method for genotyping is simple and conveniat,the prevalent HBV strain in Guangzhou is genotype B and genotype C.
Cite
Citations (0)
Cite
Citations (8)
The aim of the study was to determine AAT genotype by the simple DNA-based method in a group of ten Croatian families. AAT genotype was determined by PCR-RFLP in samples taken from each member of the ten families (mother, father and child/children). In the group of parents, five normal genotypes, Pi MM and fifteen Pi MZ genotypes, were detected. In the group of children, particular genotypes followed the mode of inheritance. There were eight Pi MZ, and five Pi ZZ genotypes. PCR-RFLP was found to be the method of choice for AAT genotyping. Determination of AAT genotype in family studies enables the risk of deficient allele inheritance to be followed-up and assessed. Early diagnosis of a deficient AAT genotype contributes to the success of currently widely available AAT replacement therapy.
Cite
Citations (0)
Cite
Citations (0)
Objective To establish a diagnostic method for ABO genotyping and to provide a simple and reliable technique for safe transfusion and identification of complicated blood group. Methods ABO genotyping method was developed by using multiplex-PCR-RFLP and PCR-SSP techniques. Results We developed a set of methods, which were stable and accurate for ABO genotyping. This diagnostic system were excellent for ABO genotyping after implementation. Conclusions We have established an reliable ABO genotyping diagnostic system.
Multiplex
Cite
Citations (0)
Objective Classified HBV DNA of AsC of children living in Guangzhou. Methods Using a genotyping method based on the restriction fragment length polymorphism (RFLP) of amplified segment of the HBV S region. Results Among the AsC of children living in Guangzhou ,genotype B is 62.7%,genotype C is 33.3% genotype B and C is 3.3%,only 1.7%couldn not be classified . Conclusions The method for genotyping is simple and convenient,The prevalent HBV strain of children in Guangzhou is genotype B and genotype C.
Cite
Citations (0)
Objective Using PCR-RDB to establish a new method for HBV genotyping, and to survey the distribution of HBV genotypes in the Foshan area. Methods Biotin-labeled primers for amplification of HBV region X (nt1550-1789) were used to amplify extracted HBV DNA. HBV was genotyped by hybridization of the PCR products with immobilized specific probes (genotype A to F) on C membrane. Color development was achieved by adding POD and TMB. A judgment was made according to color reactions. The reliability of this new method was verified by gene sequencing. 300 samples of HBV DNA-positive sera from the Foshan area were genotyped using this assay. Results Of the 300 sera genotyped by PCR-RBD, 147 (49.0%) cases were genotype B, 136 (45.3%) were genotype C, 1 (0.3%) genotype D, and 12 (4.0%) were mixtures of genotype B and C, and 4 (1.3%) were mixtures of genotype C and D. No genotype A, E or F were found. The results of PCR-RDB genotyping were consistent with the results obtained with sequence analysis. Conclusion This newly established HBV genotyping system proved to be sensitive, specific, precise and economic, and should be suitable for clinical practice and epidemic study. The results of HBV genotyping show that genotype B and C are the predominant genotypes in the Foshan area.
Cite
Citations (3)
Abstract Aggressive periodontitis (AgP) is a specific form of periodontal disease, with rapid destruction of the tissues supporting the teeth in otherwise young healthy individuals. We recently showed a higher frequency of the interleukin‐4 (IL‐4) −34TT and −590TT genotype in AgP patients compared to controls ( P < 0.05). Herein, we demonstrated that this specific IL‐4 genotype exerts its function by increasing expression of IL‐4 and STAT6, and producing higher concentrations of IL‐4 in activated CD4+ cells of patients with AgP. In the present study, we investigated the effects of the IL‐4‐specific genotype on IL‐13, IL‐2 and IFN‐γ expression and production in activated CD4+ cells of patients with AgP and healthy controls. Results revealed higher IFN‐γ and IL‐2 expression and significantly increased IL‐13 production in the cells of the patients who were homozygous for the −34T and −590T alleles in comparison with the patients who were homozygous for the −34C and −590C alleles ( P < 0.05). Results of controls with the −34C and −590C alleles were similar to those of AgP with the same genotype. To our knowledge, the present study is the first to show an effect of the −34TT and −590TT genotype on IL‐13 production. There is an increased production of IL‐13 by the T cells of aggressive periodontitis patients with the IL‐4 genotype.
Aggressive periodontitis
Cite
Citations (14)
Summary This paper provides an investigation into some of the key practical issues for minimizing the cost of DNA testing. Previous studies focused on maximizing the utility of genotyping by prioritizing individuals for genotyping. For logistical reasons, individuals may have to be genotyped in groups rather than individually, and the best group to genotype is expected to differ from the same‐sized group chosen when individuals are genotyped sequentially. In a calibration step, simulated populations and full knowledge of genotypes were used to discover the best group(s) to genotype. The characteristics of these groups were then targeted in an optimization step, using normally available information for group formation in targeted populations. Contrasts were made among predictive indices for: (i) individuals, with genotyping between each individual; (ii) individuals, with genotyping occurring group‐at‐a‐time; and (iii) groups, using group variables as criteria. The results of this investigation allow the determination of the value of moving from individual to group genotyping, reveal the favourable attributes of individuals for group formation, and lead to methods to form groups for genotyping. The approach used has applications in reducing genotyping costs in both experimental and commercial populations for both quantitative trait loci (QTL) detection and monitoring.
Cite
Citations (0)