Flow cytometric analysis of BCL‐2 can distinguish small numbers of acute lymphoblastic leukaemia cells from B‐cell precursors
18
Citation
30
Reference
10
Related Paper
Citation Trend
Abstract:
Summary Flow cytometric identification of small numbers of precursor B‐cell acute lymphoblastic leukaemia (B‐ALL) cells in post‐treatment marrow specimens could benefit from the identification of additional, easily detectable markers that could be used in most cases. In this study, we evaluate whether bcl‐2 expression quantified by four‐colour flow cytometry can be effectively used to discriminate precursor B‐ALL blasts from normal B‐cell precursors (haematogones) and function as a leukaemia‐specific marker. Levels of bcl‐2 in the 22 precursor B‐ALL cases studied were found to be significantly higher (over sixfold higher on average) than those present in haematogone populations from 22 control marrow specimens. Higher relative levels of bcl‐2 expression in the B‐ALL cases were maintained with respect to both immature CD34 + and more mature CD34 − haematogone subsets, and appeared stable. Dilutional studies indicated that multiparameter flow cytometry analysis using bcl‐2 could identify precursor B‐ALL blasts representing as few as 1% of CD19 + cells or 0·01% of total leucocytes in bone marrow specimens containing substantial numbers of haematogones. This study suggests that bcl‐2 may be an important marker for flow cytometric detection and quantification of small numbers of residual precursor B‐ALL cells in bone marrow specimens.Keywords:
Acute lymphocytic leukemia
Flow cytometry를 이용하여 개 정자의 생존율 평가를 수행하고자 2-4세의 수캐 5두가 이용되었고, 분석을 위해 PI염색을 실시하였다. Flow cytometry를 이용한 개 신선 정액의 생존율 평가는 생존 정자와 죽은 정자의 비율을 1:0, 1:1, 1:3으로 조성하여 이를 flow cytometry로 평가하고 광학현미경검사, CFDA/PI 염색검사, HOS test에 의한 생존율과 비교하여 flow cytometry와의 상관관계를 알아보았다. 또한 개 정액을 동결하여 응해 후의 개 정자의 생존율 평가에도 동일한 방법으로 상관관계를 조사하였다. 신선 정액에서 생존 정자와 죽은 정자의 비율이 1:0, 1:1, 1:3 모든 경우에서 flow cytometry를 이용한 생존율은 HOS test에 의한 생존율과 높은 상관관계를 나타내었다 (p<0.01). 신선 정액에서 생존 정자와 죽은 정자의 비율이 1:0과 1:3일 때 광학현미경적 검사에 의한 생존율은 flow cytometry 분석에 의한 생존율과 유의 적인 상관관계를 나타내었으나 (p<0.05), 1:1 비율의 경우 상관관계를 보이지 않았다. 신선 정액에 생존 정자와 죽은 정자의 비율이 1:0과 1:1일 때 CFDA/PI 염색 검사에 의한 생존율은 flow cytometry분석에 의한 생존율과 높은 상관관계를 보였으며(p<0.01), 1:3 비율에서는 유의적인 상관관계를 보였다 (p<0.05). 동결 및 응해 후의 개 정자의 생존율 평가에서 HOS test 결과는 flow cytometry분석에 의한 생존율과 높은 상관관계를 보였으며 (p<0.01), 광학현미경적 검사를 통한 생존율은 유의적인 상관관계를 보였으나 (p<0.05), CFDA/PI 염색 검사결과는 상관관계를 보이지 않았다. 이상의 결과 flow cytometry는 신선정액 및 동결ㆍ융해 후 개 정자에 대한 생존율 검사에 정확한 평가 방법 인 것으로 판단되었다.
Cytometry
Cite
Citations (0)
Abstract Simultaneous monitoring of biomarkers as well as single‐cell analyses based on flow cytometry and mass cytometry are important for investigations of disease mechanisms, drug discovery, and signaling‐network studies. Flow cytometry and mass cytometry are complementary to each other; however, probes that can satisfy all the requirements for these two advanced technologies are limited. In this study, we report a probe of lanthanide‐coordinated semiconducting polymer dots (Pdots), which possess fluorescence and mass signals. We demonstrated the usage of this dual‐functionality probe for both flow cytometry and mass cytometry in a mimetic cell mixture and human peripheral blood mononuclear cells as model systems. The probes not only offer high fluorescence signal for use in flow cytometry, but also show better performance in mass cytometry than the commercially available counterparts.
Mass cytometry
Cytometry
Cite
Citations (3)
To analyse the amounts of various cellular components in cultured mammalian cells by flow cytometry, we tried to reduce the autologous fluorescence in human-human hybridoma HB 4 C 5 cells producing IgM. The autologous fluorescence of the cells decreased after repeated rinsing with phenol red-free MEM medium at 4°C before and after ethanol-fixation. The method could be useful in the analyses of various cellular components by flow cytometry.
Cytometry
Cite
Citations (0)
Flow cytometry analysis was applied to the measurement of Epstein-Barr virus (EBV) induction which is used as a short term assay for anti-tumor promoters. The data obtained by measurement with flow cytometry were parallel with those of the fluorescence microscopic method. Flow cytometry is rapid, quantitative and should be applicable for the EBV activating test.
Cytometry
Cite
Citations (0)
Isolation
Characterization
Cite
Citations (0)
The determination of antibody binding capacity is crucial in the development of drug antibodies. Despite its widespread use, flow cytometry may only be used to evaluate single cells in solution. In addition, analyzing the antibody localization in tissue sections of animal models after drug administration, as in immunohistochemistry (IHC), is desirable. In this study, antibody-treated cells were fixed on glass slides, and their analysis was directly compared with flow cytometry. Cell-bound antibodies were quantitatively detected using a method that relies on phosphor-integrated dots (PIDs), which are highly brilliant nanoparticles applicable to immunohistochemistry. The outcomes of this approach were highly correlated with flow cytometry, suggesting that it is able to quantitatively detect cell-bound antibodies at a level comparable with flow cytometry. Therefore, this novel IHC-based technique is valuable for quantitative analysis and localization of antibodies in tissue sections of drug administration.
Cytometry
Cite
Citations (1)
Cite
Citations (20)
Late relapse of childhood lymphoblastic leukemia 10 years after the end of treatment is rare. A young woman whose acute lymphoblastic leukemia recurred 11 years after the end of treatment for acute lymphoblastic leukemia is described, and the literature on late relapse of childhood acute lymphoblastic leukemia is reviewed.
Acute lymphocytic leukemia
Cite
Citations (3)
Immunoprecipitation
Cytometry
Cite
Citations (11)
Abstract We have determined the DNA content, the ploidy levels, and the percentages of different cell types present in small and large mouse mammary tumors as well as in young and old mouse livers by using absorption and flow cytometry. Absorption cytometry data indicated a significant increase in the proportion of transformed G0/G1 cells in the tumors as compared to that of the stromal G0/G1 cells with progressive tumor growth. This increase was not detected by flow cytometry. In both young and old mouse livers, a small number of cells of higher ploidy (8C and 16C) were detected by absorption cytometry but were not apparent in histograms obatined by flow cytometry. Furthermore, changes in the proportions of liver cells of different ploidy with age were apparent in absorption cytometry data but not in flow cytometry data. In one mouse liver experiment, a 6C cell peak appeared in the flow cytometry histogram, but a direct measurement of DNA content by absorption cytometry failed to detect cells with such a peak. We therefore believe that some caution may be warranted in the use of flow cytometry alone for evaluation of DNA distributions and of the proportions of different types of cells in complex solid tissues.
Cytometry
Cite
Citations (11)