Effect of powdered gloves, worn at the time of embryo transfer, on the pregnancy outcome of IVF cycles
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Single Embryo Transfer
Single Embryo Transfer
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Objective To study the embryo quality and pregnancy rate of single embryo transfer(SET)cycles.Methods Data of 797 SET cycles including 59 fresh cycles with elective single embryo transfer(eSET),542 fresh cycles with compulsory single embryo transfer(eSET)and 196 frozen-thawed cycles were collected.The pregnancy rate between eSET and eSET cycles,fresh and frozen-thawed cycles and also the pregnancy rate in the embryos with different number of blastomere were analyzed.Results The grade of embryo was significantly related to pregnancy rate.The pregnancy rate was significantly higher in the embryos with 7-9 cells comparing with the embryos with 4-5 cells on day 3(P0.05).Conclusions The clinical pregnancy rate is closely related to the quality of embryo in the SET cycles,eSET can be applied to women and have satisfactory outcomes.
Single Embryo Transfer
Embryo quality
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Single Embryo Transfer
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Single Embryo Transfer
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To study the relationship of the number and quality of embryos transferred with clinical pregnancy rate and multiple pregnancy rate in frozen-thawed embryo transfer (FET) cycle.Retrospective analysis of the clinical data of 995 FET cycles. Patients were stratified as age < 35 or > or = 35, Both groups' clinical pregnancy rate and multiple pregnancy rate were compared according to the number of embryos and high-quality embryos transferred.(1) The clinical pregnancy rates were 50.0%, 56.6% and 56.5%, and twin pregnancy rates were 6.3%, 43.8% and 30.6% respectively in < 35 group when one, two or three embryos were transferred. There is not any significantly statistic difference among these subgroups (P > 0.05). However, the twin pregnancy rate in one-embryo transferred subgroup was significantly lower than that of two-embryo transferred subgroup (P < 0.05). Moreover, the clinical pregnancy rate was not different among single high-quality embryo transferred subgroup and other high-quality embryo transferred subgroups, the multiple pregnancy rate was lower than the others. (2) The clinical pregnancy rates were 0, 47.3% and 53.8%, and twin pregnancy rates were 0, 25.7% and 25.7% respectively in > or = 35 group when one, two or three embryos were transferred. The clinical pregnancy rate and twin pregnancy rate between two-embryo and three-embryo transferred subgroups were not significantly different (P > 0.05). Moreover, the clinical pregnancy rate was not different among two-embryo transferred containing one high-quality embryo subgroup and other high-quality embryo transferred subgroups, the multiple pregnancy rate was lower than the others.There is a close relationship between the number and quality of embryos transferred and clinical pregnancy rate, multiple pregnancy rate in FET cycles. For the patient aged < 35, we recommend performing single high-quality embryo transfer in order to reduce the multiple pregnancy. For the patient aged > or = 35, two-embryo transfer containing one high-quality embryo is enough to obtain a satisfied clinical pregnancy rate and an acceptable multiple pregnancy rate.
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This study aimed to evaluate the effect of oestrous synchrony between donors and recipients and the embryo quality on the pregnancy rate in beef cow recipients. The experiment was performed over two years at an embryo transfer (ET) centre in Southern Brazil. Ninety Aberdeen Angus cows were subjected to superovulation (SOV) protocols, resulting in the recovery of 1,048 transferable embryos. Eleven groups were formed with intervals of 6 hr, from -30 to +30 hr, with respect to recipient versus donor oestrous detection. Evaluation of embryo quality was according to the IETS guidelines. The overall pregnancy rate was 52%. Effects related to donor and recipient oestrous synchronization on pregnancy rate were observed (p = .01), ranging from 36% to 50%. The embryo quality rate affected the pregnancy rate, where Grade I resulted in 57% and Grade III in 43% of pregnancy (p < .001). The embryonic state (frozen or fresh) showed no (p > .05) effect on pregnancy rate: 53% for fresh embryos and 44% for frozen embryos. The odds ratio for explanatory variables causing pregnancy indicated that Grade III embryos had 31% less chance of conception compared to Grade I. Thus, oestrous synchrony between donor and recipient, considering ±30 hr apart, can affect the pregnancy rate along with embryo quality.
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Blastocyst Transfer
Assisted Reproductive Technology
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Assisted Reproductive Technology
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Objective:To analyze the clinical pregnancy outcomes of non-elective single embryo transfer and dis-cuss the feasibility of elective single embryo transfer(SET).Methods:A retrospective analysis was carried out on in vitro fertilization-embryo transfer(IVF-ET) cycles of Center for Reproductive Medicine in Nanfang Hospital between January 1,2002 and August 31,2007.Only one embryo was available to transfer in 202 cycles,to analyze the relations of age,ovarian response,number of embryo blastomere,and clinical pregnancy rate.Results:The average clinical pregnancy rate was 19.8% in the 202 SET cycles.The pregnancy rates were 30.8%,19.4%,16.3%,and 9.5% in 30 yr,30～35 yr,36～40 yr,and 40 yr group,respectively,and there was no statistically significant difference(P=0.192).The pregnancy rates were 20.0% and 17.6% in normal response and low response with no statistically significant difference(P=0.673).The pregnancy rates were 7.7%,12.7%,15.6%,and 34.9% in the 2～3 cell embryo group,4～5 cell embryo group,6 cell embryo group,and 7～8 cell embryo group,respectively,and there was statistically significant difference among them(P=0.002).Conclusions:The clinical pregnancy rate closely related to the quality of embryo in the SET cycles,and the highest in the 7～8 cell embryo groups.For the ovarian normal response,elective SET can be applied to women under 35 years,and have satisfactory outcomes.
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