Differential effects of divalent cations on specific 3H-GR 65630 binding to 5-HT3 receptors in rat cortical membranes
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1 Concanavalin A: An Introduction.- I. Background Information.- II. Physical and Chemical Properties of Concanavalin A.- III. Biological Properties of Concanavalin A.- References.- 2 Structure and Function of Concanavalin A.- Abstract.- I. Introduction.- II. Structure of Concanavalin A.- III. Saccharide Binding Function of Concanavalin A.- IV. Multivalent Nature of Con A.- V. A Model for Interactions between Cell Surface Receptors and Cytoplasmic Structures.- References.- 3 Studies on the Combining Sites of Concanavalin A.- Abstract.- I. Introduction.- II. Methods, Results, and Discussion.- References.- 4 13C NMR Studies of the Interaction of Concanavalin A with Saccharides.- Abstract.- I. Introduction.- II. Methods.- III. Results and Discussion.- References.- 5 Self-Association, Conformation and Binding Equilibria of Concanavalin A.- Abstract.- I. Introduction.- II. Methods, Results, and Discussion.- III. Conclusion.- References.- 6 Studies on the Interaction of Concanavalin A with Glycoproteins.- Abstract.- I. Introduction.- II. Methods.- III. Results.- References.- 7 Interaction of Concanavalin A with the Surface of Virus - Infected Cells.- Abstract.- I. Introduction.- II. Methods, Results, and Discussions.- References.- 8 Concanavalin A as A Quantitative and Ultrastructural Probe for Normal and Neoplastic Cell Surfaces.- Abstract.- I. Introduction.- II. Methods.- III. Results and Discussion.- References.- 9 Microtubular Proteins and Concanavalin A Receptors.- Abstract.- I. Introduction.- II. Methods.- III. Results.- IV. Discussion.- References.- 10 Effects of Concanavalin a on Cellular Dynamics and Membrane Transport.- Abstract.- I. Introduction.- II. Methods of Procedure.- III. Results.- IV. Discussion.- References.- 11 The Characteristics of Succinylated Con a Induced Growth Inhibition of 3T3 Cells in Tissue Culture.- Abstract.- I. Introduction.- II. Methods.- III. Results.- IV. Discussion.- References.- 12 Cell Cycle Dependent Agglutinability, Distribution of Concanavalin a Binding Sites and Surface Morphology of Normal and Transformed Fibroblasts.- Abstract.- I. Introduction.- II. Materials and Methods.- III. Results.- IV. Discussion.- References.- 13 Concanavalin A and Other Lectins in the Study of Tumor Cell Surface Organization.- Abstract.- I. Introduction.- II. Methods.- III. Results.- IV. Discussion.- References.- 14 Modification of the Biological Activities of Concanavalin A by Anti-Concanavalin A.- Abstract.- I. Introduction.- II. Methods, Results, and Discussion.- References.- 15 Concanavalin A as A Probe for Studying the Mechanism of Metabolic Stimulation of Leukocytes.- Abstract.- I. Introduction.- II. Materials and Methods.- III. Results.- IV. Discussion.- References.- 16 Enhanced Cytotoxicity in Mice of Combinations of Concanavalin A and Selected Antitumor Drugs.- Abstract.- I. Introduction.- II. Materials and Methods.- III. Results.- IV. Discussion.- References.- 17 Effect of Concanavalin A and Phytohemagglutinin on the Modification of Immunogenicity of Canine Kidney Allografts.- Abstract.- I. Introduction.- II. Materials and Methods.- III. Results.- IV. Discussion.- References.- Abstracts.- Evidence for Conformational Changes in Concanavalin A upon Binding of Saccharides as Determined from Solvent Water Proton Magnetic Relaxation Rate Dispersion Measurements.- Magnetic Resonance Studies of Concanavalin A: Location of The Binding Site of Methyl-D-Mannopyranoside.- The Metal Ion Requirements of Concanavalin A.- The Kinetics of Cellular Commitment during Stimulation of Lymphocytes by Concanavalin A.- Isolation of A Glycoprotein Receptor for Concanavalin A from the Outer Surface of Mouse L Cells.- Electron Microscopic Study on Interaction of Concanavalin A with Mouse Lymphosarcoma Cells in Tissue Culture and in Ascites form.- The Effect of Glutaraldehyde Fixation on the Agglutination of Human Erythrocytes by Concanavalin A and Soybean Agglutinin.- Altered Net Cation Transfer Across the Ehrlich Mouse Ascites Tumor Cell During Exposure to Concanavalin A.- Effects of Con A on Frog Nerve and Muscle.- Modification of the Surface Characteristics of Developing Hemopoietic Cells from Normal Human Bone Marrow Revealed Ultrastructurally by the Concanavalin A-Peroxidasediaminobenzidine Technique.- Effects of Succinyl-Con A on the Growth of Normal and Transformed Mouse Fibroblasts.- Endotoxin-Like Activities in Concanavalin A Preparations.- Concanavalin A Induced Inflammation.- Enhanced Immunogenicity of Con A Coated El-4 Leukemia Cells.- List of Contributors.
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Concanavalin A covalently bound to acrylic polymer particles (enzacryl AA), which cannot enter the cells, stimulated rat lymphocytes into DNA synthesis. Evidence was obtained that concanavalin A binds to the lymphocyte membrane specifically through its sugar‐binding sites. No nonspecific adsorption was found. The equilibrium constant found for the binding of concanavalin A to the cell was 12 μ −1 at 37°C and 3° −l at 0°C. No heterogeneity in the affinity of the binding sites was detected. The average maximum number of concanavalin A molecules (Mol. wt 108000) that could be bound was about 5 ×10 6 per cell. Optimum stimulation of DNA synthesis was obtained when about 5%, of the available sites on the cells were occupied by concanavalin A.
Binding constant
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Splenocyte
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Dictyostelium discoideum amoebae have two classes of binding sites for concanavalin A, one accessible to a competitive inhibitor of concanavalin A binding and one inaccessible. Uptake of concanavalin A occurs and is associated with a transfer from accessible to inaccessible binding sites. Low amounts of concanavalin A bound to cells produce a slight stimulation of differentiation, and this is associated with an increase in production of phosphodiesterase. Above a critical amount of concanavalin A bound, cell differentiation and phosphodiesterase production are inhibited. The relationship of concanavalin A-induced membrane changes to cell differentation is discussed.
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Abstract: In the present study results are reported concerning the effects of several divalent cations on the binding characteristics of [ 3 H]‐cyclohexyladenosine on A1 adenosine receptors and of [ 3 H]‐N‐ethylcarboxamidoadenosine on non‐A1/non‐A2 sites in membranes from cerebral cortex of the rat. The [ 3 H]‐cyclohexyladenosine binding to A1 receptors was dose‐dependently increased by Mn 2+ , Co 2+ , Ca 2+ . The binding characteristics of the agonist were differently affected by Ca 2+ /Mn 2+ and Mg 2+ . Ca 2 and Mn 2+ increased the B max value without any change in K d , whereas Mg 2+ decreased the K d value without changing the B max . In the presence of Ca 2+ and Mg 2+ the K d value was similar to that obtained in the presence of Mg 2+ , whereas the B max value was similar to the apparent number of binding sites calculated in the presence of Ca 2+ . The cations, Cu 2+ , Cd 2+ , Zn 2+ , decreased the Al binding with IC 50 , values of 19.6 μM, 39.2 μM and 103.9 μM, respectively. The binding characteristics of [ 3 H]‐N‐ethylcarboxamidoadenosine to non‐A1/non‐A2 sites were affected by Ca 2+ , Mn 2+ , Co 2+ and Mg 2+ in the opposite manner to A1 receptors. They decreased the binding with IC 50 values of 20.1 mM, 22.8 mM, 93.0 mM and 18.1 mM, respectively. This occurs through an enhancement in K d , values without changes in the number of binding sites. The findings on A1 receptor and non‐A1/non‐A2 binding site, taken together, suggest that cations could also exert a modulatory action via specific interactions with divalent cation binding sites on the receptor molecule.
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U937 cell
Respiratory burst
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Agglutination (biology)
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Using concanavalin A labeled with tritium and fluorescein isothiocyanate we studied the binding properties of this plant lectin to adult paired schistosomes. Using concanavalin A coupled to a sepharose column we attempted to isolate and characterize concanavalin A binding molecules from the epidermis of adult schistosomes. Our results indicate the presence of specific concanavalin A binding sites on the surface of adult Schistosoma mansoni. A significant percentage of the concanavalin A was specifically bound and showed characteristics similar to that identical in other concanavalin A binding tissues. The parasite's concanavalin A binding sites appear to be 2 or 3 high molecular weight glycoproteins. There is some indication that glycoproteins associated with the worm's epidermis function as enzyme(s). The immunological significance of these glycoproteins has not been determined.
Epidermis (zoology)
Fluorescein isothiocyanate
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