Three Dimensional Imaging of Maurer's Clefts in “Unroofed” Plasmodium Falciparum-Infected Erythrocytes by Transmission Electron Microscopy
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Intracellular parasite
Erythrocyte which is infected by Plasmodium falciparum will have various changes on its architecture, affinity, and biomolecular. Beside that, the infected erythrocyte also forms a knob at its surface. This knob are contained with various parasite proteins, one of them is Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP-1). Our previous study had been identified a protein with molecular weight 270 kDa at P. falciparum infected erythrocyte from Malang isolate that was playing a role in cytoadherence process. The aim of this study was to detect the possibility of 270 kDa protein expression at complicated malaria falciparum patient erythrocyte membrane. The method that used was immunocytochemistry with polyclonal antibody to 270 kDa protein. The results showed that two (2) erythrocyte samples from healthy people as control had negative reaction, and so did with five (5) erythrocyte samples of uncomplicated malaria patient, but there was positive reaction that shown at two (2) samples of complicated malaria patient erythrocyte. It can be concluded from the results that 270 kDa membrane protein of P. falciparum infected erythrocyte in complicated malaria patient might be a PfEMP-1. This protein can be detected by immunocytochemistry method using polyclonal antibody and can be used for the candidate of complicated malaria diagnostic
Keywords: Plasmodium falciparum, 270 kDA protein, immunocytochemistry, polyclonal antibody
Polyclonal antibodies
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Six culture-adapted knob-positive Plasmodium falciparum parasites, four of which were nonbinding in an in vitro cytoadherence assay, were tested for the presence of the knob-associated histidine-rich protein PfHRP1. Two knob-positive, strongly cytoadherence-positive P. falciparum strains from Aotus monkeys were also examined. All parasites expressed PfHRP1, suggesting that it plays a structural or functional role related to knobs but is not by itself sufficient for cytoadherence of P. falciparum-infected erythrocytes.
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Objective To determine the effect of proliferation of lymphocytes stimulated by Plasmodium falciparum HGXPRT protein. Methods Lymphocytes from BALB/c mice immunized with Plasmodium falciparum HGXPRT protein were prepared and the proliferation of lymphocytes was determined by CCK-8 after jointly cultured with corresponding stimulators. Results Effects of stimulation depended on the concentration of Plasmodium falciparum HGXPRT and the concentration of 5μg/ml was the most efficacious for the proliferation of lymphocytes. Conclusion Plasmodium falciparum HGXPRT can stimulate the proliferation of lymphocytes.
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