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    The effect of insulinomimetic agents on protein degradation in H35 hepatoma cells
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    Keywords:
    Proteolysis
    Protein Degradation
    Oxidizing agent
    Mechanism of Action
    A simple and sensitive fluorimetric method for determination of phenylhydrazine was described. This method was based on the inhibitory effect of phenylhydrazine on the reaction of pyronine Y with nitrite in sulfuric acid medium. The detection limit for phenylhydrazine was 7.2 ng/mL, the linear range of the determination was 8.0~60 ng/mL. The method was used to determine phenylhydrazine in water samples with satisfactory results.
    Linear range
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    Reactions of various 3-aroylmethylene-indol-2-ones with hydrazine and phenylhydrazine under exactly similar conditions have been carried out. The reaction with phenylhydrazine has not been investigated earlier. It was found that although the reaction with hydrazine hydrate afforded a spiro derivative viz., spiro[3H-indole-3,3'-(3H)pyrazol]-2(1H)-one, that with phenylhydrazine yielded simply a hydrazone derivative. Representative spiro compounds have been screened for antifertility activity but none was found active at a dose of 10 mg/kg in adult female rats.
    Hydrazine (antidepressant)
    Hydrazone
    Derivative (finance)
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    Abstract The compound obtained by Hess, the condensation product of phenylhydrazine and ω‐chloroacetophenone has been investigated and its properties can be explained by assigning to it the constitution 1: 3‐diphenyl‐(1: 2‐diazacyclobutene‐2). The structure of the compound obtained by Scholtz from phenylhydrazine and ω‐chloroacetophenone, has been elucidated and proved to be the bis‐phenylhydrazone of diphenacyl.
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