57. Anxiety about relationships is linked to alterations in cortisol production and cellular immunity
Lisa M. JaremkaR. GlaserTimothy J. LovingWilliam B. MalarkeyJeffrey R. StowellJanice K. Kiecolt‐Glaser
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Cellular immunity
Objective To study the anti-tumor effect of Huai Er,a Chinese prepared medicine,on the cellular immune function.Methods Forty cases of post-operative gastric cancer patients were divided into treatment group and control group randomly.Huai Er and 5-Fu were used respectively.Peripheral blood samples in the 40 patients were collected to detect the lymphocyte subsets during pre-operatively,2 weeks,6 weeks and 10 weeks after operation,respectively.The data were statistically analyzed by paired samples.Results The CD3+,CD3+CD4+,CD3+CD4+/CD3+CD8+,and NK cells increased remarkably 6 weeks and 10 weeks post-operatively compared with 2 weeks post-operatively(P0.05)in treatment group.Conclusions Huai Er may produce some anti-tumor effect by improving cellular immune function.
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Objective: To investigate the changes of tumor infiltrating T lymphocyte subsets in HCC patients.Methods: Obtained fresh liver tissues from thirty patients with primary liver cancer and thirty patients who underwent hepatectomy because of benign lesions,then prepared infiltrating lymphocytes from these tissues.Used the method of flow cytometry to detect the cell surface molecules of T lymphocytes: CD3,CD4 and CD8.Results: 1)The proportions of CD3+CD4+T lymphocytes in infiltrating lymphocytes in HCC tissues were(22.31±3.68)%,which were significantly higher than those in adjacent liver tissues(10.69±2.47)% and in normal liver tissues(4.21±4.26)%(P=0.000,P=0.001).The proportions of CD3+CD4+T lymphocytes in adjacent liver tissues were significantly higher than those in normal liver tissues too(P=0.019).2)The proportions of CD3+CD8+T lymphocytes in infiltrating lymphocytes in HCC tissues and in adjacent liver tissues were(26.10± 5.82)% and(21.82±2.7)%,which were significantly lower than those in normal liver tissues(41.31± 14.01)%(P=0.014,P=0.004).The proportions of CD3+CD8+T lymphocytes were similar between those in HCC tissues and in adjacent liver tissues(P=0.651).3)The ratio of CD3+CD4+T lymphocytes and CD3+CD8+T lymphocytes in infiltrating lymphocytes in HCC tissues(0.91±0.30)were significantly higher than those in adjacent liver tissues(0.47±0.11,P=0.003)and in normal liver tissues(0.11±0.13,P=0.000).Conclusion: The proportions of CD3+CD4+T lymphocytes in tumor infiltrating lymphocytes of HCC tissues increased evidently,while the proportions of CD3+CD8+T lymphocytes decreased markedly.The ratios of CD3+CD4+/CD3+CD8+T lymphocytes were evidently raised.
Tumor-infiltrating lymphocytes
Liver Cancer
Liver tissue
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Aim To investigate the effect of Panax Quinquefolium Saponin (PQS) on the cell immune system of the patients with Chronic Pulmonary Heart Disease (CPHD) and then to find out the relationship between the immune system and the mechanism of CPHD. Methods The T lymph subgroup and NK (CD3 -/CD16+56 +) cells in patients’ peripheral blood were detected by flow cytometry, and the expression of IL 2, IFNγ mRNA were analyzed by RT PCR. Results CD3 +, CD4 + and CD4/CD8 in patients with CPHD were significantly lower than those in the control, while CD8 + higher ( P 0 01). CD3 +, CD4 +, CD4/CD8 and NK (CD3 -/CD16+56 +) cells in the patients were increased after PQS treated, and CD3 +, CD4 + were significantly different from pre treated ( P 0 05), while CD8 + cells were significantly lower than those in pre treated ( P 0 05). PQS stimulated the expression of IL 2, IFNγ mRNA excreted by T lymph cells. Conclusions The results showed that PQS can improve the cell immune function, especially in patients with CPHD whose immune function are low.
CD16
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Objective To determine the cellular immunological abnormalities in aplastic anemia(AA). Methods ①The quantity of the subsets of T lymphocyte and CD8~+HLA-DR~+ cells in peripheral blood of AA patients at diagnosis were detected respectively by FACS.②Th1 and Th2 cells in peripheral blood mononuclear cells(PBMNC) of AA patients were counted by FACS. Results ①In aplastic anemia patients,the percentage of CD3~+CD4~+ cells,CD3~+CD8~+cells,CD8~+HLA-DR~+cells and the ratio of CD4~+/CD8~+ were (35.05±9.47)%,(36.29±6.81)%,(18.36±2.07)% and 1.0±0.33 respectively.In normal controls,they were (52.84±4.03)%,(24.69±3.90)%,(11.13±1.14)% and 2.14±0.24 respectively.The percentage of CD3~+CD4~+ cells and the ratio of CD4~+/CD8~+ of AA patients were significantly lower than that of controls;the percentage of CD3~+CD8~+ cells and CD8~+HLA-DR~+ cells were significantly higher than that of controls;while the percentage of CD3~+cells had no difference between AA patients and normal controls.②The percentage of Th1 cells and the ratio of Th1/Th2 of AA patients were significantly higher than that of controls,the percentage of Th2 cells had no difference between AA patients and normal controls.③The percentage of Th1 cells in AA patients were positively correlated with their CD3~+CD8~+cells and negatively correlated with their RC and ANC. Conclusion Disbalance of T lymphocyte subsets and increases in quantity of Th1 cells might be important for the development of bone marrow failure in aplastic anemia.
Aplastic anemia
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【Objective】To assay the expression of CD3~+、CD4~+、CD8~+ on T lymphocytes in patients with malignant carcinoma and investigate the relationship between T lymphocyte subset and the development and prognosis of malignant tumor.【Methods】APAAP methods were performed to detect the level of T lymphocyte subpopulation of 223 cases with malignant tumor and 220 cases of control group at the same time.【Results】The CD3~+、CD4~+、CD8~+T cell and the ratio of CD4~+/CD8~+ were significantly lower in patients than those in the control group(P0.01),while no significant difference of the number of CD8~+ T cell was found(P0.05).However,after treatment,the CD3~+、CD4~+、CD4~+/CD8~+ increased dramatically.【Conclusion】So to detect the value of CD3~+、CD4~+、CD8~+T cell and the ratio of CD4~+/CD8~+ can reflect the immunological state of malignant tumor patients and also there is some clinical value in estimating the prognosis of those patients.
Tumor-infiltrating lymphocytes
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Objective To observe the therapeutic effect of integrated therapy and the impact on T-lymphocyte subpopulation in Vitiligo patients. Methods 98 vitiligo patients were divided into integrated treatment group consisted of 65 cases and routine treatment group consisted of 33 cases, and then compared them with 25 healthy controls. The levels of T-lymphocyte subpopulation and NK cell in the peripheral blood of all patients were determined with flow cytometry. Results Effective treatment rates in treatment group, treatment control group were 75.38% and 42.42%(P0.001).Before the treatment, decrease of CD3, CD4 and CD4/CD8(P0.05) were found in two Vitiligo groups. After the treatment, CD3, CD4 and CD4/CD8 were increased in integrated treatment group compared with that of before treatment (P0.05), while there no marked change in treatment control group was observed. Conclusion Cellular immunity is impaired in vitiligo patients. Integrated treatment with thymus peptide can increase the cure rate and enhance cellular immunity.
Vitiligo
Cellular immunity
Therapeutic effect
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In 93 persons aged 90 to 103 years old the subpopulation compositon of lymphocytes and the level of thymic serum activity (TSA) was studied. In the presence of polymorbidity the low level of TSA was observed in 81% of examinees, with the quantitative deficiency in the relative and absolute number of CD3+CD8+ T-lymphocytes (62% and 78% respectively) and the excess in the number CD3+CD4-CD8- T-lymphocytes (42% and 57% respectively). Among CD3+CD4-CD8- T-lymphocytes cells with phenotypes CD3+/-CD4-CD8-CD16- and CD3+CD4-CD16+ were determined. In prolonged imunomonitoring the reciprocacity of changes in the amount of CD3+/-CD4-CD8-CD16- T-lymphocytes was established in relation to the level of TCA and the number of CD3+CD8+ T-lymphocytes. The reversibility of these changes is indicative of the potential of reactivity of the immune system in longlivers. The positive result of laser therapy in persons with quantitative deficiency of CD3+CD8+ T-lymphocytes and the presence of cells with phenotype CD3+/-CD4-CD8-CD16- was associated with the restoration of the size of CD3+CD8+ subpopulation and a decrease in an amount of CD3+/-CD4-CD8-CD16-. The unfavorable prognostic sign was, seemingly, the quantitative deficiency of CD3+CD8+ T-lymphocytes with the low level of TCA in the absence of CD3+/-CD4-CD8-CD16- T-lymphocytes.
CD16
Cellular immunity
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In order to study the immune modulation system in SLE patients, a comparative study of T lymphocyte subsets (CD3 +,CD4 +,CD8 +,CD4 +/CD45Ra +),NK cell(using flow cytometry) and sIL-2R(using ELISA method) in sixty SLE patients and thirty controlled subjects was conducted. The results showed that in SLE patients, CD8 + lymphocytes were significantly increased; while CD4 +,CD4 +/CD45Ra +,NK cell were significantly decreased; CD3 + was unaffected; sIL-2R was significantly increased. In conclusion, the risk of active SLE patients was correlated to the dysequilibrium of lymphocyte immune modulation.
Immune Modulation
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