Shape-dependent electron transfer kinetics and catalytic activity of NiO nanoparticles immobilized onto DNA modified electrode: Fabrication of highly sensitive enzymeless glucose sensor
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Keywords:
Non-blocking I/O
Glucose oxidase
Nanorod
Linear range
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The principle,character and classification of enzyme biosensor are introduced.The ways to achieve electric signal of the first generation biosensor,the superiorities of the second generation biosensor with medium and the methods and materials to fixate enzyme of the third generation enzyme biosensor are expounded.The present conditions of the third generation enzyme biosensor are commented on.Finally,the progressive directions of biosensor have also been prospected.
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Non-blocking I/O
Tandem
Hydrothermal Synthesis
Morphology
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我们准备了硅石， SiO2 涂的 NiO 和 NiO 由大音阶的第五音胶化的涂的 SiO2 方法。需要的材料的物理化学的性质被表面充电性质调查，扫描电子显微镜学(SEM ) ，精力散 X 光检查(EDX ) 光谱学，表面区域大小和 X 光检查衍射(XRD ) 分析。固体的零费用(PZC ) 的点被盐增加方法决定。在涂的材料，二 PZC 价值被注意代表他们的对应物的表面费用。， SiO2 涂的 NiO 的 SEM 图象在 NiO 涂的 SiO2 的情况下在 NiO 的表面上显示硅石的一致涂层，象外观一样的一个蜂房与高度多孔的结构被观察。在 NiO 的 diffractograms，然而，典型山峰在 NiO 涂的硅石被压制没有衍射山峰能在 SiO2 涂的 NiO 被看见。批吸附技术被申请从水的答案的 Pb2+ 离子的移动。为 Pb2+ 离子的吸着趋势在 NiO 涂的 SiO2 的顺序被观察 > SiO2 涂的 NiO > NiO > SiO2。这个趋势证实涂的材料比他们的父母对应物有更多的吸着能力。
Non-blocking I/O
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Love waves, a variety of surface acoustic waves (SAWs), can be used to detect very small biological surface interactions and so have a wide range of potential applications. To demonstrate the practicality of a Love wave SAW biosensor, we fabricated a 155-MHz Love wave SAW biosensor and compared it with a commercial surface Plasmon resonance (SPR) using glycerol-water solution with known densities and viscosities to calibrate the response signals of the biosensors. And the mass per unit area of anti-mouse IgG bound with protein G onto the sensitive layer of the biosensor was calculated on the basis of the calibration result. The sensitivity of the Love wave SAW biosensor was the same as or greater than that of the SPR biosensor. Furthermore, the Love wave SAW biosensor was capable of measuring a much wider range of viscosities than the SPR biosensor. Although the operating principle of the Love wave SAW biosensor is completely different from that of the SPR biosensor, the subtle changes in the viscoelastic properties of the biological layer that accompany biological binding reactions on the sensitive layer can be monitored and measured in the same ways as with the SPR biosensor.
Love wave
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担体付のNiO, WO3, NiO-WO3触媒上で,エチレンとプロピレンの共二量化反応を行ない,シリカおよびアルミナの担体効果と共二量化反応の機構を推察した。反応は常圧,固定層流通系で行なった。NiO-WO3-Al2O3触媒が共二量化反応に有効であり,NiO-Al2O3より活性が大きかった。また,WO3-Al2O3上では共二量化反応は起こらなかった。また生成物の異性化反応も合わせて検討し,共二量化反応による一次生成物はn-ペンテン(1または2)であり,イソペンテンはn-ペンテンの異性化によるものと結論した。また,エチレン,プロピレンの各種触媒に対する反応性は エチレン:NiO..WOs.A1203>NiO-A1203 WO3-A120s rO プピレソ:NiO-WOs-AI203>WO3-A1203>NiO-A120sであった。またこれらの触媒は二量化活性ばかりでなく,異性化活性も有し,とくにNiO-Al2O3, NiO-WO3-Al2O3は二重結合の異性化能が大きく,生成物はほとんど平衡組成に近かった。
Non-blocking I/O
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Basic aspects and examples of biosensors, including definitions and applications, response of enzyme-based biosensors, examples of biosensor configurations, ferrocene-mediated amperometric glucose sensor, potentiometric biosensor for phenyl acetate, potentiometric immunosensor for digoxin, evanescent-wave fluorescence biosensor for bungarotoxin, optical biosensor for glucose based on fluorescence energy transfer, piezoelectric biosensor for nucleic acid detection, enzyme thermistors, evaluation of biosensor performance.
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Non-blocking I/O
Partial pressure
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采用水热法，制得了管径约为10~15 nm、管长约为10~300 nm、管壁上附着NiO纳米颗粒的TiO2纳米管复合光催化剂（NiO/TiO2）.采用X射线衍射、透射电子显微镜、扫描电镜、X射线光电子能谱及紫外-可见漫反射光谱等技术对催化剂进行了表征；以甲基橙为模拟污染物，评价了纳米管的光催化活性.结果表明：NiO晶粒与TiO2晶粒结合形成p-n异质结，有效地促进了光生电子和空穴的分离；NiO对可见光有强烈的吸收，使复合TiO2纳米管在整个可见光区域均有很好的光吸收；以上两点使NiO/TiO2纳米管可见光下的光催化活性大幅提升，500℃煅烧后纳米管对甲基橙1 h分解比由纯TiO2纳米管的7.0%提升至NiO/TiO2纳米管的95.6%.
Non-blocking I/O
Hydrothermal Synthesis
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