Variability in platelet responses to collagen — comparison between whole blood perfusions, traditional platelet function tests and PFA-100
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Adenosine diphosphate
Objective To study the effects of Morroniside on platelet aggregation in rabbits induced by Adenosine diphosphate(ADP),Arachidonic acid(AA),Platelet activating factor(PAF).MethodsThe platelet aggregation was determined by Born's method.ResultsCompared with the controls,Morroniside inhibited platelet aggregation induced by ADP,AA,PAF(P0.001)and especially selective on ADP.ConclusionMorroniside inhibited platelet aggregation in rabbits,especially induced by ADP.
Adenosine diphosphate
Platelet-activating factor
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OBJECTIVE: To explore the effect of Huangzhi oral liquid on platelet aggregation.METHODS: The platelet aggregation in rabbits was determined by turbidimetry and the bleeding time of mice was measured. RESULTS: Huangzhi oral liquid inhibited the platelet aggregation induced by adenosine diphosphate (ADP), arachidonic acid (AA) or PagVR (P0.01) and prolonged the bleeding time of mice.CONCLUSION: The platelet function can be inhibited by Huangzhi oral liquid.
Adenosine diphosphate
Turbidimetry
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Purpose To investigate the effects of Rongban Tongmai Granules on platelet aggregation in rabbits.Methods Anticoagulants,such as Adenosine diphosphate(ADP),Arachidonic acid and collagen,were utilized to promote platelet aggregation,and the effects of Rongban Tongmai Granules on platelet aggregation in rabbits were observed using Born′s method,compared with those in the control groups and in aspirin groups.Results By intragastric administration of Rongban Tongmai Granules for 7 days,they reduced the platelet aggregation rates that collagen(P0.05-0.01),ADP(P0.05) and AA induced.Conclusion Rongban Tongmai Granules can inhabit platelet aggregation in rabbits,and the study has provided the experimental basis of them for treatment of patients with ischemic heart diseases.
Adenosine diphosphate
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Turbidimetry
Adenosine diphosphate
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Measurement of in vitro induced platelet aggregation by turbidimetric method is a very important investigative tool when patients are evaluated for bleeding diasthesis due to possible underlying platelet functional disorders. As commercial controls are not available, it is important that each laboratory establishes its own normal pattern of platelet aggregation induced by standard aggregating agents. The aggregation curves in terms of maximal % aggregation, Vmax and lag time in 50 normal Chinese controls are reported. The final concentrations of the aggregating agents used, namely adenosine diphosphate, adrenaline, collagen and ristocetin, are 20 microM, 10 microM, 0.2 mg/ml and 1.0 mg/ml, respectively. 20% of the controls had either no aggregation or only primary aggregation with adrenaline. Lag time by collagen-induced aggregation is significantly longer, but other parameters of platelet aggregation by adenosine diphosphate, adrenaline and collagen in Chinese are comparable to one reported control study. The maximal % aggregation induced by ristocetin in Chinese does not differ significantly from the reported values in whites but is significantly higher than in Blacks at a concentration of 1.0 mg/ml. Interpretation of platelet aggregometry in patients with bleeding tendency should be based on the normal patterns established from a group of normal controls in each laboratory rather than by comparison with a single control alone.
Ristocetin
Adenosine diphosphate
Adenosine triphosphate
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Human blood
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Adenosine diphosphate
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Adenosine diphosphate
Ex vivo
Human blood
Platelet-poor plasma
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Efficient hemostasis in human body depends on a complicated series of events which actively involve blood platelets. Platelets exhibit diverse responses in a variety of agonists. In vivo, most of the agonists act in synergism, causing aggregation of platelets.In this study, the synergism between ADP-Epinephrine and Epinephrine-Collagen has been determined by means of turbidometric method, which measures changes in optical density of platelet suspension. The study was carried out at the department of Hematology, Armed Forces Institute of Pathology, Rawalpindi. The subthreshold concentrations of each of the agonists were established with the help of dose response curve. By adding these agonists in subthreshold concentrations, the synergism between ADP-Epinephrine and Epinephrine-Collagen was determined.The combinations of these agonists in subthreshold levels showed the synergistic responses in causing platelet aggregation.These findings indicate that the optimal platelet aggregation does occur between the above mentioned pairs of agonists even when each of the agonist is added to the other in subthreshold doses.
Adenosine diphosphate
Ristocetin
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ADP-induced platelet aggregation of normal individuals and of patients with unstable angina were studied. Abnormalities of platelet function included increased aggregability. The ADP concentration required for half-maximal aggregation was significantly lower (4.28±0.30μM) in the unstable angina patients compared with that for normal individuals (5.24±0.15μM). The results are suggestive of platelet aggregation as a useful prognostic indicator for unstable angina.
Unstable angina
Adenosine diphosphate
Stable angina
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