Enhancement of an anti‐tumor effect of interferon by dipyridamole in established human malignant melanoma cell lines
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Abstract Enhancement of the anti‐proliferative effect of human interferon (HuIFN) preparations (α, β and γ) by dipyridamole was detected in a human malignant melanoma cell line, MMICB, which we originally established. Cell growth was inhibited by HulFN alone, but a marked increase in inhibition was noted in vitro and in vivo when dipyrydamole was added. Cellular DNA synthesis, as dletermined by 3 H‐deoxythymidine incorporation into the acid‐insoluble cellular fraction, was more inhibited by combined treatment than by any of the agents used alone. Two other melanoma cell lines that we established, MM‐2CB and MM‐3CB, also exhibited sensitivity to combined treatment both in vitro and in vivo. Furthermore, the HMV‐I and SEKI melanoma cell lines were susceptible to the combination. Even non‐cytotoxic concentrations of dipyridamole could enhance the effect of HulFN on MM‐ICB, MM‐2CB, and SEKI cells.Keywords:
Dipyridamole
Thymidine
Interferon alfa
Objective To discuss the method and the clinical value of dipyridamole stress test as a substitute for exercise test in ~(99m)Tc-MIBI myocardial perfusion imaging in coronary heart disease(CHD).Methods The resting tomography imaging of myocaidial was performed in 50 patients (30 patients with coronary heart disease,20 cases in control group),then the dipyridamole perfusion imaging was performed after 48 h.Results Tweenty nine of the 30 patients with CHD showed positive in dipyridamole ~(99m)Tc-MIBI myocardial perfusion imaging,and 16 of the 20 patients withont CHD showed normal images.Only one in the studies was discontinued due to dipyridamole side effect.The sensitivity of this test was 96.7%(29/30) and the specificity 80%(16/20).Conclusion Dipyridamole myocardial perfusion imaging is a safe and sensitive method in diagnosis of CHD.
Dipyridamole
Myocardial imaging
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Dipyridamole is a vasodilator that is used primarily in clinical practice as an antiplatelet agent. It increases coronary blood flow and was originally introduced as an antianginal agent. An ability to prolong a shortened platelet survival has been used to justify its value in preventing thromboembolic complications. Conditions characterized by a reduction in platelet survival and where dipyridamole has been used include heart valve replacement, arterial grafting, cerebrovascular disorders, and disorders of peripheral circulation. The in vivo effect of dipyridamole on platelet aggregation has not been well defined and may depend on additional factors. Prostaglandins appear to have important roles in platelet homeostasis; their relationships to the action of dipyridamole are discussed. Dipyridamole usually is combined with aspirin for synergistic anti-aggregatory purposes. However, the nature of the interaction has not been elucidated and benefit from the addition of dipyridamole has not been demonstrated in clinical studies. A review of clinical studies using dipyridamole indicates that it currently has limited value.
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Dipyridamole
Stress testing (software)
Thallium
Coronary angiogram
Coronary vasodilator
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The laboratory examination of Dipyridamole concerning its antiaggregation effect is presented in the paper. Thirty patients with coronary insufficiency have been taking Dipyridamole for one year. The average dosage was four pills à 75 mg of Dipyridamole a day. The aggregation and adhesion of the platelets with the examined patients was checked before and during the treatment. The inhibition platelet aggregation with Dipyridamole has been confirmed in vitro.
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Pill
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Dipyridamole stress is the forerunner and prototype of pharmacological stress echo tests in the diagnosis of coronary artery disease. Among the various stress echo tests, it is probably the least technically demanding to perform and the easiest to interpret. Its accuracy is similar to dobutamine stress echocardiography but its feasibility is higher. The prognostic impact of dipyridamole stress echo has also been proven for presentation of hard end-points such as cardiac death. The safety and prognostic value of this test has been conclusively demonstrated as a result of extensive experience in large scale multicentre projects. Dipyridamole stress is many different tests in one: dipyridamole—atropine is best for diagnosis; dipyridamole—dobutamine or dipyridamole—exercise is highly sensitive for the detection of minor forms of coronary artery disease; low and high dose dipyridamole is best suited for prognostic stratification; infra-low dipyridamole with low dose dobutamine administration is probably best suited for selective myocardial viability identification. Each patient should have their own test, tailored on the basis of the clinical picture and the diagnostic issue.
Dipyridamole
Stress Echocardiography
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客观 neutrophils 在 ischemic-reperfusion 损害起一个重要作用，这被知道。在这研究，我们在 neutrophils 上测试了效果和它 dipyridamole 的机制。由 neutrophils 的方法氢过氧化物(H2O2 ) 生产用发光氨被决定放大化合光和活动的百分比被观察不受禁令约束的山峰高度计算。结果 Dipyridamole 本身由刺激的 formyl-MetleuPhe (fMLP ) 生产了 H2O2 的集中依赖者抑制 neutrophils。在低集中的 Dipyridamole (0.3 渭摩尔路 L ? 1 ) 那本身仅仅稍微影响了 neutrophils，显著地在 neutrophils 上提高了腺苷的效果。在另一方面， dipyridamole 没在 neutrophils 上改变 NECA (5 鈥 ? N-ethylcarboxamidoadenosine ) 的禁止的效果。然而， propentofylline，腺苷举起的一个已知的禁止者，也得到一样的结果。结论 Dipyridamole 由刺激 fMLP 的 neutrophils 禁止了 H2O2 的生产。在低集中的 Dipyridamole 在 neutrophils 上提高了腺苷的禁止的效果。包含的机制可能由于腺苷举起上的 dipyridamole 的效果。
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To assess the feasibility, safety and usefulness of dipyridamole stress echocardiography for the detection of coronary artery disease we evaluated 194 patients (124 men, 70 women) with effort chest pain. All patients underwent electrocardiographic submaximal bicycle exercise testing and 2-dimensional echocardiography after dipyridamole injection. Echocardiographic test was considered positive when new wall motion abnormalities were observed after dipyridamole i.v. injection (0.56 mg/kg b.m.). Sensitivity and specificity of electrocardiographic exercise test and dipyridamole stress echocardiography were assessed in 37 persons who underwent selective coronary angiography. The sensitivity and specificity of dipyridamole stress echocardiography, were respectively 85.0% and 91.7% and were higher than those of exercise electrocardiography. 2-dimensional echocardiography after dipyridamole injection is a well tolerated, feasible and effective test in the diagnosis of coronary artery disease.
Dipyridamole
Stress Echocardiography
Stress testing (software)
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Objective To establish a method for mensuration of dipyridamole in Dipyridamole Tablets.Methods Taking advantage of electrode's response to dipyridamole to mensurate the content of dipyridamole in Dipyridamole Tablets.Results The electrode showed Nernst response within the range of 1.0×10-2~4.8×10-5 mol·L-1 with a slope of 40 mV/pC.The detection limit is 3.2×10-5 mol·L-1.Conclusions The method is simple,rapid and accurate,which can be used to mensurate the contents of dipyridamole in tablet.
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Interferon alfacon-1 differs from other interferon alfa preparations by only a few amino acids. In patients with chronic active hepatitis C, two comparative trials show that interferon alfacon-1 has the same activity and adverse effects as other interferons alfa. Interferon alfacon-1 is less easy to administer.
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There is limited evidence that dipyridamole is generally an effective antithrombotic agent when used alone, nor is there convincing evidence that the combination of aspirin and dipyridamole is more effective than aspirin alone, except perhaps in cerebrovascular disease. There is no consistent evidence to support the routine use of dipyridamole after coronary artery bypass grafting and in patients with occlusive peripheral vascular disease, although these remain common reasons for its use. Dipyridamole is a useful agent in ‘pharmacological stress’ testing in nuclear cardiology imaging and may be valuable when combined with warfarin in certain patient groups, such as those with prosthetic heart valves. When combined with aspirin, dipyridamole may be of value in the secondary prophylaxis of cerebrovascular disease, although further studies are clearly needed. In a significant proportion of cases, evidence‐based medicine cannot support the current widespread continued prescription of dipyridamole in cardiological practice, but the jury is still out on cerebrovascular disease.
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