RNA interference--significance and applications.
14
Citation
68
Reference
20
Related Paper
Citation Trend
Keywords:
RNA Silencing
Dicer
RNA-induced silencing complex
RNA-induced transcriptional silencing
Trans-acting siRNA
DNA-directed RNA interference
Argonaute
Cite
RNA interference (RNAi) is a phenomenon by which the double strande d RNA (dsRNA) induces targeted degradation of RNA molecules with homologous sequen ces in vitro and in vivo.RNAi is a powerful tool for silencing gene expression.E xtensive genetic and biochemical analysis revealed a two step mechanism of gene silencing by RNAi.The first step involves degradation of dsRNA into short or sm all interfering RNAs (siRNAs),19～25 nucleotides in length,by Dicer.In the follo wing step,the siRNAs are incorporated into a multiprotein RNA inducing silencin g complex (RISC),which acts on the homologous target mRNA and degrades it.RNAi i s currently used for both functional genomics and gene specific therapeutic inte rvention that target the mRNAs of pathogenic genes.In addition,the recent advanc es in siRNA design and delivery are reviewed as well.
RNA Silencing
Dicer
Trans-acting siRNA
RNA-induced transcriptional silencing
RNA-induced silencing complex
DNA-directed RNA interference
Functional Genomics
Cite
Citations (0)
RNA interference (RNAi) is the process of sequence-specific post-transcriptional gene silencing in animals and plants initiated by double-stranded RNA (dsRNA).It advanced at a miraculous pace since it was discovered in 1998. Here the mechanism and application of RNAi are reviewed.
RNA Silencing
RNA-induced transcriptional silencing
RNA-induced silencing complex
Cite
Citations (0)
RNA Silencing
Trans-acting siRNA
RNA-induced silencing complex
RNA-induced transcriptional silencing
Cite
Citations (366)
RNA interference (RNAi) is the process of sequence-specific post-transcriptional gene silencing in animals and plants initiated by double-stranded RNA (dsRNA) that is homologous in sequence to the silenced gene. RNAi in diverse organisms reveals the same highly conserved mechanism with an ancient origin. The basic process in RNAi involves a dsRNA that is processed by endonuclease into small interfering RNA (siRNA) that guides recognition and targeted cleavage of homologous target mRNA. The discovery of RNAi and the following studies on the molecular mechanism under RNAi lead to the development of new strategies for blocking gene function and may yield RNA-based drugs to treat human disease.
RNA Silencing
DNA-directed RNA interference
Trans-acting siRNA
RNA-induced silencing complex
RNA-induced transcriptional silencing
Cite
Citations (2)
RNA Silencing
Dicer
RNA-induced silencing complex
RNA-induced transcriptional silencing
Trans-acting siRNA
Argonaute
DNA-directed RNA interference
Endoribonuclease
Cite
Citations (0)
RNA interference(RNAi) is something of post-transcriptional gene silencing,specifically mediated by double-stranded RNA.RNAi was observed in many model organisms,such as Arabidopsis,C.elegans and fungi.The basicprocess in RNAi involvesa dsRNA that is processed by endonuclease into small interference RNA(siRNA) that guides recognition and targeted cleavage of homologous target mRNA.Being effective and highly specific,RNAi has becames a novel technique in knocking gene down plays important roles in gene function study and gene therapy diseases.
RNA Silencing
DNA-directed RNA interference
Trans-acting siRNA
RNA-induced silencing complex
RNA-induced transcriptional silencing
Cite
Citations (0)
A new phenomenon-RNA interference(RNAi) has been observed in many organisms recently, including eukaryote and prokaryote. When Double-stranded RNA(dsRNA) is introduced into cell, it would be decomposed to small interfering RNA(siRNA), and RNA-induced silencing complex(RISC) is formed out of siRNA and several enzymes. RISC can combine with mRNA and incise it, then the mRNA is disintegrated and the expression of gene is inhibited. The phenomenon is also called the sequence-specific post-transcriptional gene silencing(PTGS), and the process is charactered by high efficiency and peculiarity. RNAi has played an important role in the research of genic functions, and may yield RNA-based drugs to treat diseases,such as virus and tumor and genetc diseases.
RNA Silencing
DNA-directed RNA interference
Trans-acting siRNA
RNA-induced silencing complex
RNA-induced transcriptional silencing
Cite
Citations (0)
RNA silencing, named as co-suppression or post transcriptional gene silencing (PTGS) was found in transgenic plants which was the result of cellular mRNA degradation and silencing of gene expression. RNA interference (RNAi) is a specific technique using only a few double stranded RNA (dsRNA) molecules to stop the expression which has made it one of the important areas in molecular biology. By introducing a gene into the host genome which is highly homologous to an endogenous gene, the RNA silencing is initiated. Double-stranded RNA (dsRNA) is cut by the enzyme “Dicer” producing small interfering RNAs (siRNAs) which combine with RNA-induced silencing complex (RISC). RISC, a protein complex, binds one strand of siRNA with mRNA of native target gene for destruction, resulting in gene silencing. The mechanism of RNAi offers a quick and easy way to determine the function of a gene. In this review, we discuss the history, components, mechanism and the application of RNA interference.
RNA-induced silencing complex
RNA Silencing
RNA-induced transcriptional silencing
Trans-acting siRNA
Dicer
Argonaute
DNA-directed RNA interference
Cite
Citations (3)
RNA interference (RNAi) is an evolutionally conserved gene silencing mechanism present in a variety of eukaryotic species. RNAi uses short double-stranded RNA (dsRNA) to trigger degradation or translation repression of homologous RNA targets in a sequence-specific manner. Over the past decade RNA interference (RNAi) has emerged as a natural mechanism for silencing gene expression. This system can be induced effectively in vitro and in vivo by direct application of small interfering RNAs (siRNAs), or by expression of short hairpin RNA (shRNA) with non-viral and viral vectors. This review briefly describes the molecular principles underlying of RNAi phenomenon and discuss the main technical issues regarding optimization of RNAi experimental design. Keywords: RNA interference (RNAi), double strand RNA (dsRNA), short hairpin RNA (shRNA)
RNA Silencing
Trans-acting siRNA
DNA-directed RNA interference
RNA-induced silencing complex
RNA-induced transcriptional silencing
Cite
Citations (1)
RNA interference (RNAi), i.e. gene silencing, or gene expression down-regulation is the process whereby a double-stranded RNA (dsRNA) induces the homology-dependent degradation of cognate messenger RNA (mRNA). When dsRNA is introduced into cells, an RNA-induced silencing complex (RISC) is assembled. RISC serves as cellular machinery that is responsible for the specific mRNA degradation. This process results in the subsequent reduction of the specific protein translated from appropriate mRNA. Short RNA duplexes (21 nucleotide), called small interfering RNA (siRNA), have become the major tool for induction of gene silencing. With the human genome mapped and sequenced, attempts are currently being made to manipulate the expression of genes involved in viral diseases, carcinogenesis and other disorders with the aim of developing novel therapies.
RNA Silencing
RNA-induced silencing complex
Trans-acting siRNA
RNA-induced transcriptional silencing
DNA-directed RNA interference
Argonaute
Cite
Citations (0)