Aberrations of the MYC gene in unselected cases of diffuse large B-cell lymphoma are rare and unpredictable by morphological or immunohistochemical assessment
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Abstract:
Diffuse large B-cell lymphomas (DLBCL) with aberrations of MYC probably represent a distinct clinicopathological entity following an aggressive course. Their incidence in unselected DLBCL collectives is debatable and the identification of such cases may be difficult. Therefore, the molecular epidemiology of MYC aberrations in DLBCL and whether they can be predicted by morphology and immunohistochemistry were studied on tissue microarrays containing 333 cases. Evaluation of MYC by fluorescence in situ hybridisation was successful in 220/333 (66%) cases. 9/220 (4%) cases showed MYC breaks. Re-evaluation of these tumours did not show any specific morphological and/or immunohistochemical features. The median survival time was 9 months for the respective patients, as opposed to 80 for patients without MYC breaks. The presence of MYC breaks in DLBCL cannot be reliably predicted by conventional methods. Since such patients might profit from different forms of treatment, routine testing of all DLBCL for MYC aberrations is suggested.Keywords:
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'HER2-low' breast cancer is an emerging issue as the clinical trials for anti-HER2 antibody-drug conjugates (trastuzumab deruxtecan) are making progress. A reliable method to identify HER2-low cancers is needed. This study aimed to evaluate immunohistochemistry (IHC) and in situ hybridisation (ISH) in detecting HER2-low status.We evaluated the HER2 ISH data grouped by the IHC consensus in proficiency tests (PTs), and compared the HER2 ISH results between HER2 IHC scored 0 (IHC-0) and IHC scored 1+ (IHC-1+) tumours from in-house tissue microarrays (TMAs). Since benign/normal glands have HER2 expression and ideally should not be affected by targeted therapy, we evaluated HER2 ISH results in peritumoural benign glands of 52 breast cancers as reference values.None of the 565 tissue cores in PTs achieved an 80% participant agreement of IHC-1+. In PTs and in-house TMAs, HER2 signals of the IHC-1+cores (median: 2.6 and 2.0, respectively) were significantly higher than those of IHC-0 cores (median: 2.0 and 1.7, respectively). But the ranges of HER2 signals had a considerable overlap between IHC-1+and IHC-0 cores. The HER2 signals and HER2:CEP17 ratios of peritumoural benign glands exhibited normal distributions, and their upper bounds of the 95% reference intervals were 2.10 and 1.30, respectively.Current HER2 testing algorithms are unsatisfactory in detecting HER2-low cases. Using ISH to detect tumour with HER2 signals and HER2:CEP17 ratio higher than the upper bound of the benign glands can be an alternative method.
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Objective To examine the reliability of tissue microarrary(TMA) for detecting tumor marker in soft tissue sarcoma compared to the immunohistochemistry.Method Tissue microarray technology was used to detect the expression of Ki-67,PCNA and P53 in 80 cases of STS and compared the results with the immunohistochemistry.Results No difference was found between the immunohistochemistry results by 1.5 mm tissue microarray and the negligible difference was found between by traditional pathological technology and by tissue microarray in soft tissue sarcoma.Conclusion On the positive rate of Ki-67,PCNA and p53,no significant difference is found between the results with the traditional pathological investigation and with the tissue microarray(P0.05).It has important practical significance and broad application prospect in pathology.
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Aims: To improve the interpretation of immunohistochemistry (IHC) staining results the use of a tissue microarray technique was established in a routine setting. Methods: A tissue microarray was constructed by harvesting 600 μm tissue cores from paraffin wax embedded samples available in a routine pathology department. The punches originating from non-tumorous tissue were placed on host paraffin wax blocks. The microarray contained 12 different tissue samples, with a wide antigen profile and a dimension of 3.5 × 3 mm. One section of the multitissue array was placed as an "internal" positive control on each slide of the patient tissue to undergo identical immunohistochemical procedures. Results: Using the tissue microarray technique as a tool for internal quality control, the interpretation of immunohistochemical staining of more than 20 different antigens in routine IHC was improved. The tissue microarray did not influence the staining results in conventional IHC or in different automated IHC settings. Conclusion: The regular use of an institution adapted tissue microarray would be useful for internal positive control in IHC to enable different laboratory demands. Furthermore, this technique improves the evaluation of staining results in IHC.
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Objective To evaluate the significance of the appliance of tissue microarray and immunohistochemistry technique on detecting the gene expression in endometrial cancer.Methods The expressions of P16,PTEN,E-cadherin and CtBP1 of the 60 patients with endometrial cancer(experimental group) and 30 patients with normal endometrial(control group) were observed by tissue microarray technology and immunohistochemichal methods.Results The Positive expressions of P16,PTEN and E-cadherin of the patients in the experimental group were significantly higher than those in the control group(P 0.05),while there was no difference of CtBP1 expression between the two groups(P 0.05) .Besides,there were no differences of the expressions of 4 indicators of immunohistochemistry in different cancer cell differentiation(P 0.05) .Conclusion Tissue microarray and immunohistochemistry technique could be useful and reliable in the quick diagnosis of endometrial cancer in the mass samples,which indicates that there are relationships between P16,PTEN,E-cadherin and endometrial cancer and they are of reference values for pathologic diagnosis.
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Aim: To establish the tissue microarray (TMA) technique and to explore its application in immunohistochemical staining. Methods:A total of 82 samples from paraffin embedded blocks of mazoplasia and breast cancer were applied in this study.Routine 4 μm sections were cut from the TMA blocks. HE staining and immunohistochemical staining were performed. P53 protein expression was detected using TMA and rountine sections. Results: The dots on TMAs were regularly arranged and their morphology were well preserved.HE and immunohistochemical staining showed that 100% samples in paraffin section of TMA could be analyzed morphologically, which was in accordance with that of routine sections.Conclusion: The established TMA technique with manual method is feasible and economic.
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Traditional techniques in clinical diagnostic pathology have some limitations. Here we tried to develope frozen tissue microarray as a fast, simple and economical technique.A total of 48 dots frozen tissue microarray were constructed in this study including primary 19 lung cancer samples and 5 normal lung tissue samples from primary lung cancer. Hematoxylin-Eosin and immunohistochemical stains were performed on the sections and we assess the feasibility on morphology and genetic expression.The cores on frozen tissue microarray of OCT block were regularly arranged and had no shift and distort. After HE-stained, morphology of the cores was well preserved. They were answered for the request of research.Immunohistochemistry of array slides: we performed immunohistochemistry on the tumor tissue microarray with antibodies for EMA. EMA staining is uniform across the sample and gives the expected cytochylema-associated staining. There is no background staining. We compared the staining of immunohistochemistry on section of frozen tissue microarray to the results on normal slides. The results showed the accord rates were 94.7% (18/19), and there were no significant differences between them each other (P >0.05).The frozen tissue microarray is feasible. HE and immunohistochemical stains showing this methodology will be useful for morphology and immunohistochemical based protein analyses.
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The objective of this study was to investigate the relationship between BRCA1 protein expression, as determined by immunohistochemistry, and clinical outcome in uterine serous carcinoma (USC).A tissue microarray containing duplicate cores of 73 cases of USC was immunohistochemically stained with mouse anti-BRCA1 (Ab-1) mouse monoclonal (MS110) antibody. The cores were scored in a semiquantitative manner evaluating both the distribution and intensity of nuclear staining. BRCA1 protein expression was correlated with progression-free survival.Seventy-two of 73 cases were assessable, and there was a statistically significant decreased progression-free survival for those cases exhibiting tumor cell nuclei staining of 76% or greater (P = 0.0023).Our study illustrates that a low level of BRCA1 protein expression is a favorable prognostic indicator in USC, similar to what is observed in high-grade serous ovarian carcinoma. Further studies should focus on the BRCA1 status of USCs at a molecular level and also investigate whether BRCA1 protein expression is associated with response to chemotherapy in USC.
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