Intestinal Amebiasis: A Diagnosis not to be Missed
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Amoebiasis
There are a large number of species of amoebae which parasite the human intestinal tract. Of these Entamoeba histolytica / dispar is the only species found to be associated with intestinal disease. Although many people harbour this organism world wide. Amoebiasis is an infection usually caused by the pathogenic Entamoeba histolytica / dispar, and is commonly an infection of the colon. It has a world wide distribution where environmental sanitation is poor. The parasite may behave as a commensal (causing no harm to the host) or it may act as a parasite (harming the host). It is a disease of human beings, although some monkeys can become infected and the infection is then transmissible to humans.. So we used the Lemon juice ( Citrus ) at different concentration against Entamoeba histolytica to treat the amoebiasis disease. Entamoeba histolytica shows the variety of growth due to the effect of Lemon juice ( Citrus ). Lemon juice is ingredient and it has antiamoebic properties against Entamoeba histolytica a causative agent of amoebiasis.
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Antiprotozoal activity against Entamoeba histolytica of furocoumarins isolated from Ruta chalepensis
Amoebiasis caused by Entamoeba histolytica, a protozoan of the family Endomoebidae is associated with high morbidity and mortality and is therefore considered as the third parasitosis of medical importance after malaria and schistosomiasis [1, 2]. Currently metronidazole is the therapeutic drug of choice for the treatment of amoebiasis, but is experiencing drug resistance by E. histolytica, resulting in the need for increased doses to overcome the infection and thus causing unpleasant side effects. For this reason new, more effective and safer antiprotozoal agents are urgently required [3].
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Stake holders meet on "Identification and Detection of Entamoeba histolytica" was conducted on July 21, 2019 at Sri Balaji Vidyapeeth Deemed-to-be-University, Pondicherry. This programme was of national importance, since the amoebiasis is being increasingly reported from different parts of India because of poor socioeconomic conditions and sanitation levels. Experts in amoebiasis research across India attended this meeting. This meeting was conducted with an objective to frame the guidelines on the identification and detection of E. histolytica with reference to conventional diagnostic methods and molecular diagnosis targeting appropriate genes of E. histolytica. The recommendations of the panel were released as declaration on the diagnosis of amoebiasis and were circulated to various administrative and scientific bodies in India as reference policy document on the diagnosis of amoebiasis.
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Amoebiasis
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Amoebic liver abscess
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Entamoeba histolytica, the causative agent of human amoebiasis remains a significant cause of morbidity and mortality in developing countries and is responsible for up to 100,000 deaths worldwide each year. Entamoeba dispar, morphologically indistinguishable from E. histolytica is more common in humans in many parts of the world. Similarly Entamoeba moshkovskii, which was long considered to be a free-living amoeba is also morphologically identical to E. histolytica and E. dispar, and is highly prevalent in some E. histolytica endemic countries. Humans are the host of infection and there would not appear to be other meaningful animal reservoirs of E. histolytica. Entamoeba. histolytica can be present in sewage and contaminated water. The infection is mainly transmitted via ingestion of water or food contaminated by faeces containing E. histolytica cysts. Clinical features of amoebiasis range from asymptomatic colonization to amoebic dysentery and invasive extraintestinal amoebiasis, which is manifested most commonly in the form of abscesses in liver and lungs. The epidemiology of amoebiasis has dramatically changed since the separation of E. histolytica and E. dispar species and the worldwide prevalence of these species has not been estimated until recently. Morever, E. moshkovskii, another morphologically indistinguishable human parasitic Entamoeba was not mentioned or considered as a contributor to the prevalence figures in endemic areas. Amoebiasis is still a major health problem especially in aboriginal settlements and amongst people living in remote area in Malaysia. However, until now there is only one data currently available to indicate the true prevalence and incidence of E. histolytica and E. dispar. Further studies are needed to determine the burden of E. histolytica, E. dispar and E. moshkovskii infections in Malaysia. In the present review, we briefly summarize all methods use in diagnosing Entamoeba species, ranging from microscopic identification to molecular detection such as culture and isoenzyme analysis, antibody detection tests, antigen detection tests, immunochromatographic assays, conventional PCR, real-time PCR and loop-mediated isothermal amplification (LAMP).
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Amoebiasis is a food-borne protozoan infection, caused by Entamoeba histolytica. Here a case of hepatopleuropulmonary amoebiasis, which was detected after fibre-optic bronchoscopy is reported. Bronchial aspirate showed trophozoites of Entamoeba histolytica. The patient was treated with tinidazole and responded favourably.
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Amoebiasis is an infectious disease caused by Entamoeba histolytica (E. histolytica), the common symptoms are cramping, abdominal pain, watery or bloody diarrhea, and weight loss. Sometimes patients suffer amoebiasis as an asymptomatic behavior. In 1859 scientist W.D. Lambl first described the amoebiasis vector E. histolytica. In developing countries, millions of people die due to amoebiasis. A doctor diagnosed the disease detection of E. histolytic antigen in stool or antibodies against the parasite in serum. Metronidazole, tinidazole, lodoquinol, diloxanide furoate are given for amoebiasis patients. In this review, we have summarized the statistics, pathogenesis, diagnosis, prevention, and treatment strategy of amoebiasis disease. It also gives information about the life cycle of E. histolytica.
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Entamoeba histolytica is the pathogenic species of Entamoeba that causes amoebic dysentery and other invasive disease. The morphologically similar species, E. dispar, is non-pathogenic and accounts for about 90% of the previously estimated 500 million E. histolytica infections world-wide. Because of the recent redefinition of E. histolytica and E. dispar, and the limited number of drugs available to treat amoebiasis, a new approach to treatment of individuals carrying these parasites is necessary. A meeting of eminent scientists has recently agreed that on no account should prophylaxis against amoebiasis be given, and no treatment without symptoms should be administered. The expense of treating asymptomatic individuals, both monetary and at the risk of over-use of precious drugs, does not appear to be justified. It would seem wise that we preserve currently effective anti-amoebic drugs and avoid the development of drug-resistant E. histolytica.
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