Thermo-responsive multilayer films from ionic polymers with elastin-like peptide as graft chains
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Abstract:
Here we describe that graft polyions (elastin-like peptide (ELP)-grafted polyacrylic acid and polyallylamine) are successfully prepared and the ELP graft chains display thermo-responsive conformational change from hydrous coil to dehydrated β-turn structure similar to that of the polymer-free ELP. The layer thickness in the layer-by-layer films from these graft polyions is significantly affected by the deposition temperature because of the existence of ELP, and the resultant layer-by-layer films show layer-number dependent structural color changes that are completely thermo-reversible.Keywords:
Polyacrylic acid
Layer by layer
Deposition
Matrix (chemical analysis)
Fibrillin
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ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElastin mRNA levels and insoluble elastin accumulation in neonatal rat smooth muscle cell culturesLeesa M. Barone, B. Leslie Wolfe, Barbara Faris, and Carl FranzblauCite this: Biochemistry 1988, 27, 9, 3175–3182Publication Date (Print):May 3, 1988Publication History Published online1 May 2002Published inissue 3 May 1988https://pubs.acs.org/doi/10.1021/bi00409a008https://doi.org/10.1021/bi00409a008research-articleACS PublicationsRequest reuse permissionsArticle Views59Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Tropoelastin
Northern blot
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Thoracic aorta
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Abstract —The elastin content in the thoracic aorta of male Brown-Norway (BN) rats is 31.4±1.2% (dry weight), whereas that of male LOU rats is 37.2±1.0%. A similar difference in the elastin content of the thoracic aorta is also observed in female animals. Furthermore, in the thoracic aorta of young, growing rats as well as in cultured aortic smooth muscle cells, the steady-state level of elastin mRNA is significantly lower in the BN than in the LOU strain. These results suggested that 1 or more genes control the elastin mRNA level and the elastin content in the aortas of BN and LOU rats. A possible relationship between a polymorphism in the elastin gene and the elastin content of the aorta was tested. For this purpose, the aortic elastin content was measured in F 1 and F 2 generations bred from LOU and BN rats and was compared with that of the F 0 (parental) generation. A polymorphic marker located in intron 25 of the elastin gene has been used to genotype the F 2 rats. The degree of genetic determination of aortic elastin content was estimated to be 73% in the F 2 cohort, but the elastin locus accounts for only 3.9% of the total variance in aortic elastin content. Other genes are thus responsible for the major part of the observed interstrain difference by regulating the transcription of the gene, the stability of elastin mRNA, and/or posttranslational events.
Thoracic aorta
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Utilizing Layer-by-Layer (LbL) assembly, a fabrication method for the controlled creation of porous structures on Polyallylamine Hydrochloride (PAH) and Polyacrylic Acid (PAA) coatings was developed. These LbL coatings, generated through an acid corrosion process, resulted in the formation of distinctive porous structures. Experimental parameters, specifically temperature gradients from ambient to 60°C, and immersion time were systematically manipulated, enabling the successful generation of porous structures with sizes of 300 nm, 800 nm, 1200 nm, 1600 nm, and 2000 nm.
Polyacrylic acid
Layer by layer
Hydrochloride
Acrylic acid
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The relative levels of elastin-specific mRNA were used as a measure of tropoelastin expression in uteri from pregnant Sprague-Dawley rats. The levels of elastin-specific mRNA were also correlated with values for net tropoelastin production and net deposition of mature, crosslinked elastin. The total content of uterine elastin increased throughout gestation, reaching maximal levels at Day 19 of gestation, which were three times those of nongravid tissue. Following involution, the elastin content decreased rapidly to near baseline values by 5 days postpartum. The content of soluble elastin, estimated using an enzyme-linked immunosorbent assay, paralleled in part the increase in elastin deposition and elastin mRNA levels. Uterine elastin metabolism appears to be unlike that in other elastic tissues, e.g., lung and large blood vessels. In most elastin containing tissues, the protein is synthesized during discrete developmental periods and is not readily degraded. However, uterine elastin is continuously expressed, and appears to be in a continual cycle of degradation and replacement.
Tropoelastin
Desmosine
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The presence of elastin layers in the aortic walls of twelve human fetuses was confirmed with scanning electron microscope pictures after hot alkali treatment and histochemical examination. In addition, the number of elastin layers in aortic walls of 5 different segments were compared in fetuses of varying ages. Aldehyde fuchsin stained slides of elastin ascending aortas showed a range between 27 and 55 layers of elastin in fetuses of 8 weeks to 32 weeks. However, in the lower abdominal aortas, elastin layers decreased from 28 to only 3 layers for fetuses of the same age. Furthermore, as elastin layers decreased from ascending aorta to abdominal aorta with the progression of fetal life, similar changes in the elastin lamellae were observed. These results suggest that while aortas grow rapidly in length, the medial elastin thickens slowly, perhaps due to slow development of hydrodynamic forces and pressures. Also the adventitial elastin appears to lose out gradually along the length from ascending aorta to abdominal aorta.
Abdominal aorta
Elastic fiber
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The electrostatic layer-by-layer (LbL) assembled multilayer films were widely used in the biomedical technology such as drug delivery. In this work, loading capabilities and release behavior of the multilayer films chitosan (Cts) and poly(acrylic acid) (PAA) were studied. The multilayer films were assembled by LbL technique through alternating deposition of Cts and PAA on glass slides, using methylene blue (MB) as a model drug. All the results showed that the LBL film’s loading and release efficiency greatly controllable by pH and ionic strength of the solution. It suggested that the Cts/PAA LBL film had potential applications in drug delivery and controlled release studies.
Polyacrylic acid
Acrylic acid
Layer by layer
Methylene blue
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Thoracic aorta
Homeostasis
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