A cautionary note on the use of certain restriction endonucleases with methylated substrates

The restriction endonuclease BsiI from Bacillus sphaericus was isolated. The recognition sequence and cleavage point of enzyme BsiI have been determined as (sequence: see text). This restriction endonuclease is not an isoschizomer of any known restriction endonucleases and differs from other enzymes: it hydrolyses DNA into unsymmetrical recognition sequence.

Isoschizomer

Recognition sequence

Cleavage (geology)

Bacillus sphaericus

Restriction site

Sequence (biology)

Restriction fragment

Source

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Citations (2)

Type II restriction endonucleases from Helicobacter pylori include an enzyme with a novel recognition sequence

FEMS Microbiology Letters (1999)

A. Ivic

Type II restriction endonuclease activities of Helicobacter pylori strain Roberts and of the type strain H. pylori NCTC 11637 were detected and analysed by conventional techniques. The endonucleases were partially purified, their optima for activity and their recognition and cleavage sites were determined. H. pylori (Roberts) contained at least two enzymes: HpyBI was an isoschizomer of RsaI (GT/AC) and HpyBII was of a novel specificity (GTN/NAC). H. pylori NCTC 11637 was found to contain an isoschizomer of EcoRV (HpyCI: GAT/ATC) and at least one other enzyme which was too unstable to characterise.

Isoschizomer

EcoRV

Recognition sequence

Helicobacter

10.1016/s0378-1097(99)00409-7

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Citations (0)

[Specificity of restriction endonuclease VneI].

PubMed (1987)

Degtiarev SKh Rechkunova Ni Netesova Na Chizhikova Ve Malygin Eg

The recognition sequence and cleavage point of restriction endonuclease VneI have been determined as 5'-G decreases TGCAC. This enzyme is not isoschizomer of any known restriction endonucleases and therefore may be widely used in investigation of DNA structure.

Isoschizomer

Recognition sequence

Cleavage (geology)

Restriction fragment

Source

Cite

Citations (1)

The inhibition of restrication endonuclease Pcull cleavage activity by methylaytion outside its recognitionm sequecne

Nucleic Acids Research (1991)

Defeng Chen Qiang Liu Xiongwei Chen Xi-Lin Zhoa Ya‐Wen Chen

The recombinant plasmid pGEM4Z-ras DNA which was methylated on dam and dcm sites outside the PvuII recognition sequence was digested with restriction endonuclease PvuII, and one of the three PvuII sites was about 16-fold less efficient to cleave than either of the other two. On the contrary, the three PvuII sites were cleaved at about the same rate on the unmethylated DNA molecule. The results show that the cleavage inhibition of the methylated DNA on the certain PvuII site was caused by methylation outside the PvuII recognition sequence. Maybe a adjacent methylated dam site *A was responsible for the less efficient cleavage. This observation suggests that methylation outside the recognition sequence may be considered a new factor in the kinetic experiment of restriction endonuclease.

Cleavage (geology)

Recognition sequence

Cleave

10.1093/nar/19.20.5703

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Citations (9)

[Substrate specificity of restriction endonuclease Eco781].

PubMed (1985)

Viktoras Butkus R Kazlauskene R. Gilvonauskaité M Piatrushite A A Ianulaĭtis

The recognition sequence and cleavage point of restriction endonuclease Eco781 have been determined as 5'-GGCGCC-. There are several known enzymes recognizing the same sequence, although the prototype NarI and isoschizomers NdaI and NunII cleave the substrate to produce 5'-protruding ends, whereas cleavage with isoschizomer BbeI results in 3'-protruding ends. Therefore, restrictase Eco78I, generating flush ends, may be regarded as an enzyme with new specificity among the restriction endonucleases recognizing the 5'-GGCGCC-sequence.

Isoschizomer

Cleave

Recognition sequence

Cleavage (geology)

Homing endonuclease

Sequence (biology)

Source

Cite

Citations (1)

[Bsp153AI and BspM39I--new isoschizomers of PvuII restriction enzyme].

PubMed (1996)

A A Kalugin M. Rina Michael A. Eldarov В. Ф. Зарытова Maria Markaki

New restriction endonucleases, Bsp153AI and BspM39I, were isolated from Bacillus species strains 153A and M39, respectively. The enzymes recognize and cleave the nucleotide sequence [sequence: see text] and are true isoschizomers of restriction endonuclease PvuII.

Isoschizomer

Cleave

Recognition sequence

Homing endonuclease

Sequence (biology)

Source

Cite

Citations (0)

[Substrate specificity of restriction endonuclease VspI].

PubMed (1987)

Degtiarev SKh Prikhod'ko Gg Rechkunova Ni

The recognition sequence and cleavage point of restriction endonuclease VspI have been determined as 5'-AT decreases TAAT. This enzyme is not isoschizomer of any known restriction endonucleases. DNA pBR322 contains a single VspI recognition sequence in position 3539. Therefore this enzyme may be used for cloning DNA in the VspI site in AmpR-gene of pBR322.

Isoschizomer

Recognition sequence

Homing endonuclease

Cleavage (geology)

Restriction site

Source

Cite

Citations (3)

Three site-specific endonucleases from thermophilic strain Bacillus species LA are isoschizomers of HhaI, AsuII, and HindIII.

PubMed (1997)

Sergey A. Shiryaev L. A. Zheleznaya N. I. Matvienko

Isoschizomer