Variability of inflammatory mediator production by human periodontal fibroblasts stimulated with bacterial lipopolysaccharide

In Vitro Cellular & Developmental Biology - Animal (1996)

Sreenivas Koka Richard Reinhardt Constance R. Maze John K. Dyer

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Inflammatory mediator

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Oral microbiology and periodontitis research

Proteoglycans and glycosaminoglycans research

Periodontal Regeneration and Treatments

10.1007/bf02722978

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Cell trafficking, mediator release, and articular metabolism in acute inflammation of innervated or denervated isolated equine joints

American Journal of Veterinary Research (1998)

Joanne Hardy Alicia L. Bertone Steven E. Weisbrode William W. Muir Thomas M. O’Dorisio

SUMMARY Objectives To describe the acute cellular response, inflammatory mediator release, and effect on chondrocyte metabolism of interleukin 1β (IL-1β) in isolated innervated or denervated equine metacarpophalangeal joints. Animals One metacarpophalangeal joint of 24 adult horses. Procedures The metacarpophalangeal joint was isolated for 6 hours in a pump-perfused, auto-oxygenated, innervated or denervated metacarpophalangeal joint preparation. Isolated joints were assigned to 4 groups: control, control-denervated, inflamed, and inflamed-denervated, and inflammation was induced by intra-articular injection of IL-1β. Synovial fluid was collected for cytologic examination and determination of IL (IL)-1β, (IL-6), prostaglandin E 2 (PGE 2 ), and substance P (SP) values. Synovial membrane was immunostained with SP and nerve-specific enolase (NSE) antibodies. Cartilage was collected for determination of proteoglycan (PG) synthesis and degradation. Results IL-1β induced significant neutrophilic leukocytosis in synovial fluid and synovial membrane. IL-1β concentration had returned to baseline by 5.5 hours, but IL-6 concentration significantly increased throughout the study. Total SP content was significantly higher in inflamed joints. There was a significant increase in 24- and 48-hour PG degradation in inflamed innervated joints. Conclusion Cellular response to IL-1β was rapid and sustained; joint clearance of IL-1β was rapid, and endogenous production of IL-1β did not follow. The IL-6 and PGE 2 concentrations significantly increased, and SP content was increased in association with inflammation but not denervation. A degradative response of cartilage to IL-1β was observed, and was enhanced by innervation. This model was useful for investigation of the articular response to acute inflammation and the influence of denervation in modulating this response. ( Am J Vet Res 1998;59:88–100)

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10.2460/ajvr.1998.59.01.88

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[Leukotrienes promote inflammation and proliferation. Interaction with other mediator systems--modulation by therapeutic measures].

PubMed (1998)

Claudio Denzlinger

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