EFFECT OF SPLENECTOMY BEFORE BONE-MARROW TRANSPLANTATION ON SURVIVAL IN CHRONIC GRANULOCYTIC LEUKAEMIA
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Debulking
Homing (biology)
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To study the effect of hot and humid environment (HHE) on the bone marrow and spleen in mouse exposed to gamma irradiation.After exposure to gamma irradiation at the dosage of 6.5 Gy, the mice were subjected to treatment in HHE for 1 h or at room temperature (RT) respectively. After exposure to HHE, the mice in HHE group were kept at RT. The bone marrow and spleen of the mice in both RT and HHE groups were examined at 1, 3, 5, 9 and 15 days after irradiation for bone marrow cellularity, DNA content in the bone marrow cells, and spleen/body weight ratio.Compared with the RT group, mice in the HEE group showed aggravation of decreases in bone marrow cells, DNA content and spleen/body weight ratio.HHE aggravates radiation injury of the bone marrow and spleen in mice.
Gamma Irradiation
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Debulking
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ABSTRACT After transplantation into rats lethally treated with cytotoxic chemicals both bone marrow and spleen CFU in the spleen and spleen derived CFU in the bone marrow expand with doubling times ( T d ) of approximately 18 hr. However, bone marrow derived CFU in the bone marrow have a T d of 36 hr. Evidence obtained using tritiated thymidine in vitro and methotrexate in vivo show that the proliferation rate of bone marrow derived CFU is similar in both the bone marrow and spleen and calculations suggest that the different T d between these two sites is due to the higher loss of CFU through differentiation in the bone marrow compared to the spleen. These findings further support the hypothesis of an environment in the spleen which favours CFU self‐maintenance over differentiation with the opposite situation occurring in the bone marrow.
Thymidine
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Bone marrow, spleen and liver of healthy and erythroleukemic mice have been studied by autoradiography. A peripheral zone, where the most of cells were involved in mitosis, and a central one, where the mitotic index was lower, were established in the bone marrow. Normally a zone of high proliferative activity was found in the subcapsular region of the spleen. Boundaries of such zones in the bone marrow and spleen of erythroleukemic mice disappeared. Most of the cells forming the leukemia infiltrates in the liver are in the phase of DNA-synthesis.
Mitotic index
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The occurrence of plaque-forming cells (PFC) in mouse bone marrow was studied during primary and secondary responses to the thymus-independent antigen Escherichia coli lipopolysaccharide (LPS). Anti-LPS responses were induced by various doses of LPS. During the primary response, doses of 1 and 10 mug LPS intravenously (i.v.) were found to evoke a distinct PFC response in both spleen and bone marrow. The spleen contained the majority of PFC until about 5 days after immunization. During the course of the reaction the number of PFC in the bone marrow rose to a level which equalled or surpassed the level in the spleen. LPS doses of 0-001, 0-01 and 0-1 mug i.v. only induced a PFC response in the spleen. Apparently there is a minimal threshold dose of LPS of about 1 mug for PFC to appear in the bone marrow. The secondary response was studied in mice primed with 1 mug LPS i.v. and boosted with either 0-001, 0-1 or 10 mug LPS i.v. 3 months later. After each dose tested the PFC activity in the spleen was several times higher than during the primary response. As was observed in the primary response doses of 0-001 and 0-1 mug LPS i.v. did not evoke a PFC response in the bone marrow. After boosting with 10 mug of LPS i.v. a significant PFC response was found in spleen, bone marrow, thymus, lymph nodes, Peyer's patches and blood. From about 5 days after the booster injection the number of PFC in the bone marrow exceeded the total number found in all other lymphoid organs. The results are discussed in relation to the bone marrow PFC response to the thymus-dependent antigen sheep red blood cells. To this antigen a clear PFC response in the bone marrow is found only during the secondary response.
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