A case study in converting disposable process scouting devices into disposable bioreactors as a future bioprocessing tool
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In this study, we perform mass transfer characterization (k(L) a) on a novel mechanically driven/stirred Process Scouting Device, PSD, (SuperSpinner D 1000®, SSD) and demonstrate that this novel device can be viewed as disposable bioreactor. Using patch-based optical sensors, we were able to monitor critical cell culture environmental conditions such as dissolved oxygen (DO) and pH in SSD for comparison to a 1 L standard spinner (SS) flask. We also coupled these mass transfer studies with mixing time studies where we observed relative high mixing times (5.2 min) that are typically observed in production scale bioreactors. Decreasing the mixing time 3.5-fold resulted in 30% increase in k(L) a (from 2.3 to 3.0 h(-1) ) and minimum DO level increased from 0% to 20% for our model hybridoma cell line. Finally, maximum viable cell density and protein titer stayed within ±20% of historical data, from our standard 5 L stirred bioreactor (Biostat®) operated under active DO control.Keywords:
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Abstract Two different designs of stirred‐tank bioreactor, the ‘conventional’ continuously stirred‐tank bioreactor (CSTR, 42 dm 3 and 300 dm 3 ) and a horizontal‐loop bioreactor (TORUS, 114 dm 3 ) were used for the cultivation of Xanthomonas campestris , and their performances with respect to oxygen‐transfer rates and xanthan production were compared. The strictly aerobic yeast Trichosporon cutaneum was also cultivated in the TORUS bioreactor in a synthetic medium with up to 3% (w/v) xanthan added. Xanthan solutions are pseudoplastic and therefore an apparent viscosity at a shear rate (y) of 28.8 s −1 was used to compare the rheological behavior of the different test media. Maximal apparent viscosities of 1100mPa s were measured with xanthan concentrations between 20 and 25 g dm −3 . With apparent viscosities of up to 800 mPa s, the performance of the TORUS bioreactor was found to be equivalent to the performance of the CSTR bioreactor in terms of oxygen transfer and xanthan production rates. However, the amount of glucose converted to xanthan was greater in the TORUS bioreactor. Since the power consumption for the TORUS is lower compared to that for the CSTR, this new design is an interesting alternative to the CSTR for the production of xanthan.
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This chapter explores the use of bioreactors for cell multiplication in cultured meat product development. The chapter begins by first providing an overview of the principles and structure of a bioreactor, which is followed by a breakdown of bioreactor operation modes: batch cultivation, fed-batch cultivation and continuous cultivation. The chapter also highlights the important bioprocess parameters that need to be considered when creating a stem cell niche in a bioreactor, such as temperature, oxygen, pH level, lactic acid and culture mixing. The chapter considers cultivation anchorage-dependent cells in stirred tank bioreactors, the scale-up of a bioprocess in bioreactors and also highlights areas which could help reduce cost and optimise processing within bioreactors.
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Bioprocessing addresses the phases, developments, and process unit operations required to take a technology from the conceptual or benchscale stages of development to the pilot, demonstration, and commercial development stages. These critical steps in technology development o ften stand between the invention and the ultimate goal of commercial success.
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This review is related to bioreactors for plant suspension culture and its products. Bioreactor plays an important role in bioprocess engineering. The core of bioprocessing technology is the bioreactor. A bioreactor is basically a device in which the organisms are cultivated and helps in production of desired products in a contained environment. Bioreactors are usually a containment which provides optimal condition for microorganisms in order to produce desired products. In this review, the bioreactor’s principle, working and its types are discussed. Enclosed by unit operations that carry out physical changes for medium preparation and recovery of products, the reactor is where the major chemical and biochemical transformations occur. In many bioprocess, characteristic of the reaction determined to a large extent the economic feasibility of the project. The integration of biosynthesis and separation is considered as a possible approach towards more efficient plant cell and tissue culture. In this review article, the aspects of bioprocess engineering for plant suspension culture and its products, bioreactor types, optimized strategies for production of secondary metabolites also and its industrial applications.
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