Antibacterial properties of silver nanoparticles synthesized by marine Ochrobactrum sp.
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Metal nanoparticle synthesis is an interesting area in nanotechnology due to their remarkable optical, magnetic, electrical, catalytic and biomedical properties, but there needs to develop clean, non-toxic and environmental friendly methods for the synthesis and assembly of nanoparticles. Biological agents in the form of microbes have emerged up as efficient candidates for nanoparticle synthesis due to their extreme versatility to synthesize diverse nanoparticles with varying size and shape. In the present study, an eco favorable method for the biosynthesis of silver nanoparticles using marine bacterial isolate has been attempted. Very interestingly, molecular identification proved it as a strain of Ochrobactrum anhtropi. In addition, the isolate was found to have the potential to form silver nanoparticles intracellularly at room temperature within 24 h. The biosynthesized silver nanoparticles were characterized by UV-Vis spectroscopy, transmission electron microscope (TEM) and scanning electron microscope (SEM). The UV-visible spectrum of the aqueous medium containing silver nanoparticles showed a peak at 450 nm corresponding to the plasmon absorbance of silver nanoparticles. The SEM and TEM micrographs revealed that the synthesized silver nanoparticles were spherical in shape with a size range from 38 nm - 85 nm. The silver nanoparticles synthesized by the isolate were also used to explore its antibacterial potential against pathogens like Salmonella Typhi, Salmonella Paratyphi, Vibrio cholerae and Staphylococcus aureus.Keywords:
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Abstract In this work, surface plasmon resonance (SPR) was investigated over the effect of concentration of metal precursor, concentration of reducing agent, reaction time and pH on formation of silver nanoparticles (AgNPs) using biological method. In this method, Murdannia loriformis extract (MLE) was used as reducing agent and silver nitrate as metal precursor. SPR of Murdania loriformis silver nanoparticles (MLE-AgNPs) was measured through UV-vis spectrophotometer over a range of 380 – 800 nm wavelength. It was found that, as the concentration of MLE, pH and reaction time increases, the SPR peak of MLE-AgNPs at 430 - 490 nm also increases. However, for effect of concentration of metal precursor, the result showed that SPR peak of MLE-AgNPs increases when 1 to 5 mM of silver nitrate was used but decreases for 10 and 15 mM. Significant of this study is to identify the optimum conditions for synthesis of MLE-AgNPs.
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Synthesis and antibacterial activity of silver nanoparticles (AgNPs) using tyrosine as a capping and reducing agent has been performed.The purpose of this research was to understand the effect of silver nanoparticles on antibacterial activity.AgNPs synthesis was performed by combining silver nitrate solution (AgNO3) as a precursor to amino acid tyrosine and heating it in a boiling water bath until the appearance of a color shift from colorless to yellow.Antibacterial activity test of silver nanoparticles (AgNPs) was performed with the bacteria Bacillus subtilis and Escherichia coli using the well diffusion method in solution.The well diffusion method was carried out by making a hole in the agar base media with a diameter of 6 mm.TEM characterization showed the formation of AgNPs has been successful.It was also known that the AgNPs have a round shape with an average particle size of 13.628 nm.AgNO 3 solution had antibacterial activity greater than silver nanoparticles.Ag + ions were converted into silver nanoparticles to reduce the toxic properties of Ag + so that it was safe for human health.The antibacterial activity in solution has a stronger effect against Bacillus subtilis (gram-positive) than Escherichia coli (gram-negative).
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This quasi experimental study was designed to evaluate antibacterial activity of chemically synthesized silver nanoparticles (AgNPs) from silver nitrate (AgNO3) solutions on gram negative bacteria like E.Coli using disc diffusion method. Different concentrations of AgNPs, AgNO3 and reference drug ciprofloxacin were used to find out the antibacterial activity which revealed that AgNPs possessed significant antibacterial effect compared to AgNO3 solutions but relatively less antibacterial effect than that of ciprofloxacin. So chemical synthesis guided AgNPs may have some antibacterial effects.TAJ 2016; 29(2): 37-41
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Silver chloride
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Nanorod
Localized surface plasmon
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Abstract The biosynthesis of silver nanoparticles using plants as reducing agents has been reported. In this study used plant extracts Myrmecodia pendans which function as reducing agents and AgNO3 as metal precursors. Silver nanoparticles were successfully synthesized by using plant extracts of Myrmecodia pendans by doing them on variations in concentration and stirring time. In this case a variation of 0.5 mM is used; 1mM; 1.5 mM; 2 mM; and 2.5 mM and the time variation used was 120 minutes and 150 minutes. Analysis using UV-Vis spectrophotometer to determine silver nanoparticles formed in a wavelength range of 400-500 nm. From the results of the study, it was obtained that the concentration of 1 mM with 120 minutes stirring time was the best concentration and the time of formation of silver nanoparticles without stabilizer with absorbance of 2.125 at a wavelength of 408 nm and at a concentration of 2.5 mM with 120 minutes stirring time was the best concentration and stirring time formation of silver nanoparticles with a stabilizer at a wavelength of 413.5 nm with absorbance of 2,636.
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Polyvinyl Alcohol
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Synthesis of silver nanoparticles was carried out using environmentally Beluntas (Pluchea Indica L.) leaf extract to minimize the use of harmful chemicals. Synthesis was carried out with the ratio of AgNO3 solution and the beluntas leaf extract 90 mL : 1 mL. The process of forming silver nanoparticles was studied and monitored by observing the absorption spectrum using UV-Vis spectrophotometer. The observations showed the silver nanoparticles that was formed had SPR (Surface Plasmon Resonance) absorbance peak values at the wavelength of about 445,5 nm with an absorbance value of 3,437. The absorbance value increased with the increasing reaction time from 1 hour to 168 hours. From the result of testing using PSA, the average size of silver nanoparticles was 109.1 nm which is dispersed between 1,8 nm – 127,3 nm.
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There is a worldwide interest in silver nanoparticles (AgNPs) synthesize by various chemical reactions for use in applications. Silver nanoparticles have gained significant interest due to their unique optical, antimicrobial, electrical, physical properties and their possible application. However, it is necessary to develop environmental friendly methods for their syntheses. To avoid chemically toxicity, biosynthesis of metal silver nanoparticles is proposed as a cost-effective and environmental friendly alternative. This study aimed to find out whether Alagaw plant can potentially act as a reducing agent for the biosynthesis of silver nanoparticles and whether the concentration of the leaf extract can affect the absorbance spectrum, size and shape of the synthesized silver nanoparticles. The synthesized silver nanoparticles were characterized using the UV-vis spectroscopy for its absorbance spectrum and Transmission Electron Microscope Analysis for its morphology and size. The experimental method of research was used using three treatments and replicates of the different concentrations of Alagaw leaf extract: Treatment A (0.2 g/mL), Treatment B (0.4 g/mL) and Treatment C (0.6 g/mL) with 10 minutes and 60 minutes interval of observation under UV-vis spectrophotometer. Based on the findings of the study, Alagaw plant can potentially act as a good reducing agent for the biosynthesis of silver nanoparticles. The results recorded from UV-vis spectrophotometer support the biosynthesis and characterization of silver nanoparticles that as the concentration of the leaf extract increases it significantly affect the wavelength peaks and absorbance peaks of the synthesized silver nanoparticles. Using the high-resolution Transmission Electron Microscopy, the size of silver nanoparticles measured 50 nm – 100 nm having near-spherical in shape.
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Antibacterial activity using silver nanoparticles synthesized using Murraya Paniculata (L) rinds extract has been carried out. This study aims to determine the antibacterial activity of green synthesized silver nanoparticles using Murraya Paniculata (L) rinds extract. Silver nanoparticles were synthesized by reacting AgNO3 with water extract of the Murraya Paniculata (L) rinds. Green synthesized silver nanoparticles were tested for antibacterial activity using Escherichia coli ATCC 8739. Silver nanoparticles synthesized using Murraya Paniculata (L) rinds extract were characterized using a UV-Vis spectrophotometer to confirm the formation of silver nanoparticles. The UV-Vis spectrophotometer results showed the formation of silver nanoparticles which was indicated by an absorption around 400-450 nm. The antibacterial activity test results of silver nanoparticles showed a strong inhibitory activity.
Keywords: Green Synthesis, Silver Nanoparticles, Antibacterial, Echerichia Coli
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단분산성 스타이렌-염화비닐벤젠 공중합체(PSBC) 나노입자의 표면을 개질하고 AgNO3과의 반응을 통하여 Ag 나노입자가 고정화된 100-200 nm 평균 입자직경을 가지는 Ag-고분자 혼성 나노입자를 제조한다. 먼저 무유화 에멀젼 중합 방법을 이용하여 단분산성 PSBC 공중합체 나노입자를 합성한 뒤, 이들 나노입자 표면의 chlorine그룹을 thiourea 등을 이용하여 thiol기로 변화, 개질(PSBSH)한다. 이들 PSBSH 고분자 나노입자와 AgNO3와의 반응을 통하여 Ag입자가 표면에 고정화된 고분자-Ag 복합체(PSBAg) 나노입자를 제조하였다. Ag입자의 고정화 과정은 PSBSH 나노입자 표면에서 Ag+ 이온과 표면의 thiol기와의 반응을 통하여 강한 Ag-S- 결합을 형성하고 반데르발스 힘에 의해 Ag 이온들이 고분자 표면에서 환원되면서 Ag 입자가 고정화된 PSBAg 복합나노입자를 제조하였다. 고분자 표면에 은 나노 입자들이 고정된 형태의 나노 복합체의 합성과 표면구조는 SEM, TEM, XRD, FTIR, UV-Vis spectroscopy 등 다양한 분석 방법을 확인하였고, PSBAg 복합나노입자는 표면에 존재하는 Ag 입자로부터 비롯된 표면 플라즈몬 공명(SPR) 현상을 확인할 수 있었고 고분자 입자의 크기와 PSBSH 고분자에 고정된 Ag 나노입자의 함량의 변화에 따른 SPR 현상의 변화를 관찰하였다.
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