Determination of Guan-Fu Base A, a new anti-arrhythmic, in human plasma by gas chromatography and electron–capture detection

A method for analyzing belladonna alkaloids--hyoscyamine, scopolamine, anisodamine and anisodine, by means of capillary GC and GC-MSD was described. The retention data and characteristic ions of the parent and TMS derivatives of these alkaloids are given and will be very useful for identification of unknown alkaloids. The derivatization of different silylation reagents was compared, and the derivatization with MSTFA was found to be better than with BSA and BSTFA. The method has been used for studying biotransformation of these alkaloids in biosynthesis. Analysis of anisodamine and anisodine by CGC and GC-MS has not been found in the literature. In addition, the advantages of TMS derivatization are also discussed.

Anisodamine

BSTFA

Hyoscyamine

Scopolamine

Source

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Ultrasensitive analysis of microbial fatty acids using gas chromatography with electron capture detection

European Journal of Clinical Microbiology (1987)

Lennart Larsson Anders Sonesson J Jiménez

Flame ionization detector

Electron capture detector

Electron capture

Capillary gas chromatography

10.1007/bf02013086

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Citations (5)

Effects of Deproteinization and Derivatization in Serum Preparation on Serum Metabolome Based on Gas Chromatography-Mass Spectrometry

Cai Pin

The effects of different deproteinization and derivatization methods in serum sample preparation on metabolome were compared by gas chromatography-mass spectrometry.The relative standard derivations(RSD) of peak area and information content of profile were calculated.The principal components analysis(PCA),qualitative and quantitative analysis of serum samples and quality control samples(QC) were done.The RSD of QC and serum samples was less than 10%.The result of information content and PCA showed that these serum preparation methods were stable and reliable.The PCA result of profile produced by four preparation methods showed that the differences existed in serum metabolome when the samples were deproteinized and derivatizated by different reagents in preparation procedures.The influence of derivatization reagents on serum metabolome was great than that of deproteinization reagents.The information content of samples derivatizated by N-methyl-N-(trimethylsilyl) trifluoroacetamide(MSTFA)(8.9) was higher than that of samples derivatizated by BSTFA(8.7).Forty-four metabolites were obtained by qualitative analysis.Five metabolites were unique to samples derivatizated by MSTFA,and six metabolites were unique to samples derivatizated by N,O-Bis(trimethylsilyl) trifluoroacetamide(BSTFA).We proposed that when polar amino acids and short-chain fatty acids were concerned,BSTFA was recommended as derivatization reagent,and when nonpolar amino acids and long-chain fatty acids were concerned,MSTFA was recommended as derivatization reagent.

BSTFA

Metabolome

Trimethylsilyl

Sample Preparation

Quantitative Analysis

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Citations (1)

Optimization and validation of in-situ derivatization and headspace solid-phase microextraction for gas chromatography–mass spectrometry analysis of 3-MCPD esters, 2-MCPD esters and glycidyl esters in edible oils via central composite design

Food Chemistry (2019)

Minwei Xu Zhao Jin Zhongyu Yang Jiajia Rao Bingcan Chen

Solid-Phase Microextraction

Solid phase extraction

10.1016/j.foodchem.2019.125542

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Citations (24)