In vivo adaptive control of β-receptors and adenylate cyclase during short-term heat exposure in rat parotid glands

Adenylate cyclase activity in platelet membrane preparations was measured in the presence of prostaglandin E1 (PGE1), GTP and a non-hydrolysable analogue of GDP, guanosine 5'-[beta-thio]diphosphate (GDP[beta S]). A dose-dependent inhibition of adenylate cyclase by GDP[beta S] was observed that could be reversed either by adding increased amounts of GTP or of PGE1.

Prostaglandin E1

Guanosine diphosphate

BETA (programming language)

10.1042/bj2140231

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Citations (13)

N-Chloroacetylhydrazone of oxo-GTP irreversibly inhibits the activating function of GTP-binding protein coupled with adenylate cyclase.

PubMed (1985)

Perfilyeva Ea Skurat Av Yurkova Ms Khropov YuV Bulargina Tv

Guanine nucleotides are successfully used in the studies of regulatory N-proteins coupled with adenylate cyclase. In the present work N-chloroacetylhydrazones of oxo-GTP and oxo-GDP are described. After 4 hr preincubation of these nucleotides with plasma membranes from bovine brain caudate nucleus, the ability of adenylate cyclase to be activated by guanylyl-5'-methylene-diphosphonate is blocked. The degree of inhibition depends on preincubation time and increases in the presence of Mg2+. Guanylyl-5'-methylenediphosphonate protects adenylate cyclase from the action of N-chloroacetylhydrazone of oxo-GTP. These findings suggest that adenylate cyclase activation is diminished as a result of covalent modification of the Ns. N-chloroacetyl-hydrazone of oxo-GDP also causes a loss of the adenylate cyclase sensitivity to the fluoride ion and cholera toxin.

Cholera toxin

Source

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Consequences of hormone-induced desensitization of adenylate cyclase in intact cells

Biochemical Journal (1980)

S. Swillens Émilie C. Lefort R Barber R.W. Butcher J.E. Dumont

A hypothesis on the role of the hormone-induced desensitization of adenylate cyclase is proposed. It is suggested that the desensitization process could provide the cell with a highly efficient cyclic AMP system for transmitting hormone stimulus without requiring a large energy consumption. Theoretical considerations show that in fact the desensitization phenomenon allows the cyclic AMP system to present a good compromise between the efficiency and economy requirements of the cells.

Stimulus (psychology)

10.1042/bj1880169

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Citations (25)

Effects of GTP,GDP[βS] and glucose on adenylate cyclase activity of E. coli B

FEBS Letters (1985)

Janet M. Stein H.L. Kornberg Bronwen Martin

Adenylate cyclase activity was measured in suspensions of E. coli B, rendered permeable with toluene. The enzyme was activated in a dose‐dependent manner by GTP and by its non‐hydrolysable analogue, GTP[γS]. In contrast, incubation with GDP[βS], a non‐phosphorylatable analogue of GDP, caused a dose‐related inhibition of adenylate cyclase; this was partially overcome by addition of GTP. GTP did not relieve, and GDP[βS] augmented, the non‐competitive and dose‐related inhibition of E.coli adenylate cyclase by glucose.

Guanosine diphosphate

10.1016/0014-5793(85)80348-3

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Citations (9)

Regulation of the synthesis of adenylate cyclase in Escherichia coli by the cAMP — cAMP receptor protein complex

MGG Molecular & General Genetics (1981)

Irene Majerfeld Diann Miller Eva Spitz H.V. Rickenberg

cAMP receptor protein

Growth-hormone-releasing hormone receptor

10.1007/bf00428738

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Citations (44)