Comparative analysis of gastric bacterial microbiota in Mongolian gerbils after long-term infection with Helicobacter pylori
Takako OsakiTakahiro MatsukiTakashi AsaharaCynthia ZamanTomoko HanawaHideo YonezawaSatoshi KurataTimothy Derg-hoong WooKoji NomotoShigeru Kamiya
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Keywords:
Eubacterium
Prevotella
Helicobacter
Summary Significant associations have been reported between (a) specific bacterial species isolated from root canals and (b) between individual bacterial species and endodontic symptoms and signs. The prime objective of this study was to determine whether particular combinations of specific bacteria are associated with individual endodontic symptoms and signs. Seventy root canals were investigated microbiologically taking care to maintain the viability of obligate anaerobes, which accounted for 64% of the total species isolated, including Peptostreptococcus micros, Prevotella melaninogenica, Prevotella oralis, Eubacterium aerofaciens, Eubacterium lentum, Fusobacterium nucleatum, Prevotella buccae and Prevotella intermedia. Significant associations were found between individual clinical features and the following pairs of species: (a) pain (37 cases) and Peptostreptococcus spp./Prevotella spp., Peptostreptococcus spp./Prevotella melaninogenica, Pstr. micros/Prev. melaninogenica (all P < 0.01); (b) swelling (23 cases) and Pstr. micros/Prevotella spp. ( P < 0.01); (c)‘Wet’canal (57 cases) and Prevotella spp./Eubacterium spp. ( P < 0.01), Peptostreptococcus spp./Eubacterium spp. ( P < 0.05). Thus data from this investigation suggests that statistically significant associations exist between individual endodontic symptoms and signs and particular combinations of specific bacteria.
Peptostreptococcus
Prevotella
Eubacterium
Fusobacterium nucleatum
Prevotella intermedia
Fusobacterium
Obligate anaerobe
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The aim of this study is to compare between the count of Bifidobacterium spp. and Lactobacillus spp. as probiotic in stool of both autistic and non-autistic children. Bifidobacterium spp. and Lactobacillus spp. were assessed in stool samples of 40 autistic children and 20 healthy typical children of similar ages which consider as control group. The study indicated that the counts of both Bifidobacterium spp. and Lactobacillus spp. were lower in the stool of autistic children than that of their control group. It was significant in the case of Lactobacillus spp. with (p value 0.05) and highly significant in the case of Bifidobacterium spp. with (p value 0.000) and the count of bacteria wasn't affected by sex (male or female).
Bifidobacterium breve
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Objective:To observe the effect of inhibition and disinfection of Enteropathogens by Bifidobactium QJ405 and Lactobacillus QJ405 in vitro.Methods:The inhibiting test was performed on two groups:One was the testing group in which Bifidobacterium QJ405 and Lactobacillus QJ405 were cutured dlone respectively.The change of number of the bacteria had been observed at an inter of two of four hours,and the curve of bacterial grouth bad been drawn.In the disinfecting test,the metabolite of Bifidobecterium QJ405 and Lactobacillus QJ405 quantitatively inoculated respectively on the EMB agqar medium's surface which was spreaded by the quantative enteropathogens in advance,and the growing state of these bacteria was observed in the inoculated region at the following step.Results:It is obvious in our test that the grouth of hour strains of Enteropathogens and the two standard strains(ATCC2592,ATCC25923)were inhibited by Bifidobacterium QJ405 and Lactobacillus QJ405 and the merabolite of Lactobacillus QJ405 could destroy the Eneropthogens.Conclution:Bifidobacterium QJ405 and Lactobacillus QJ405 have some antibiotic effects as the probiotics.
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The nutritional and healthy solidified yoghurt containing Bifidobacterium adolescentic has been made,using fresh milk and sugar as the main raw materials and using Bifidobacterium adolescentic,Lactobacillus delbrueckii subsp.bulgaricus and Streptococcus thermophilus as starter cultures.The optimum technological condition of solidified yoghurt of Bifidobacterium adolescentic was determined:they are respectively 8% of sugar,3% of inoculum and three lactic acid bacteria which are Lactobacillus delbrueckii subsp.bulgaricus,Streptococcus thermophilus and Bifidobacterium adolescentic and the percentage of these three lactic acid bacteria is 1:1:4,the time of fermentation is 4 h.The number of Lactobacillus delbrueckii subsp.bulgaricus,Streptococcus thermophilus and Bifidobacterium adolescentic could be counted in solidified Bifidobacterium adolescentic.Solidified yoghurt contain the number of viable Lactobacillus delbrueckii subsp.bulgaricus is 8.0×10 8 cfu·mL-1,the number of viable Streptococcus thermophilus is 8.5×10 8 cfu·mL-1,the number of viable Bifidobacterium adolescentic is 2.0×10 6 cfu·mL-1.
Fermentation starter
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Bifidobacterium and Lactobacillus are beneficial for human health, and many strains of these two genera are widely used as probiotics. We used two large datasets published by the American Gut Project (AGP) and a gut metagenomic dataset (NBT) to analyze the relationship between these two genera and the community structure of the gut microbiota. The meta-analysis showed that Bifidobacterium, but not Lactobacillus, is among the dominant genera in the human gut microbiota. The relative abundance of Bifidobacterium was elevated when Lactobacillus was present. Moreover, these two genera showed a positive correlation with some butyrate producers among the dominant genera, and both were associated with alpha diversity, beta diversity, and the robustness of the gut microbiota. Additionally, samples harboring Bifidobacterium present but no Lactobacillus showed higher alpha diversity and were more robust than those only carrying Lactobacillus. Further comparisons with other genera validated the important role of Bifidobacterium in the gut microbiota robustness. Multivariate analysis of 11,744 samples from the AGP dataset suggested Bifidobacterium to be associated with demographic features, lifestyle, and disease. In summary, Bifidobacterium members, which are promoted by dairy and whole-grain consumption, are more important than Lactobacillus in maintaining the diversity and robustness of the gut microbiota.
Robustness
Dominance (genetics)
Gut bacteria
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Objective:To draw a distinct gut microbiota pattern of children with moderate-severe dust mite-induced allergic rhinitis（DAR） and healthy children. Methods:3-10 years old moderate-severe DAR children（68 cases） and healthy children（38 cases） were involved in this study. General information was collected through questionnaires, and fecal samples were collected for metagenomic sequencing. MetaPhlAn3 was used to generate the microbiota composition abundance in detail, and Alpha and Beta diversity changes were calculated. The difference in species abundance at different taxonomic levels were compared. Differences in functional pathways were compared by LEfSe analysis. Results:The diversity of gut microbiota in children with moderate-severe DAR didn't change significantly compared with healthy children. A total of 37 microbial communities or species with significant abundance difference were found, mainly included Lachnoclostridium, Prevotella, Blautia wexlerae, Prevotella copri, Eubacterium eligens, Eubacterium sp CAG 180, etc. However, the metabolism functions of gut microbiota in children with moderate-severe DAR changed compared with healthy children. Various of fatty acids anabolism enhanced in DAR children. Conclusion:Compared with healthy children, there was no significant difference in gut microbial diversity in moderate-severe DAR children. The abundance of a series of specific microbe species had a marked alteration in DAR, accompanied with changes in certain microbial functional pathways.目的：探讨中-重度尘螨变应性鼻炎（DAR）儿童的肠道微生物多样性和结构特征。 方法：选取中-重度DAR患儿68例为DAR儿童组及相匹配的健康儿童38例为健康儿童组，收集一般资料信息，采集粪便样本行宏基因组测序。使用MetaPhlAn3生成样本的菌群组成丰度表，计算Alpha及Beta多样性变化。比较两组间不同分类水平上的物种丰度差异，LEfSe分析检验组间功能通路差异。 结果：DAR儿童组肠道微生物群的物种多样性与健康儿童组比较无明显变化。相对丰度具有显著性差异的微生物群落或种属主要包括蓝绿藻菌属、普雷沃菌属、Blautia wexlerae、Prevotella copri、Eubacterium eligens、Eubacterium sp CAG 180等。中-重度DAR儿童与健康儿童肠道内的微生物功能代谢存在显著差异，DAR儿童的多种脂肪酸合成代谢增强。 结论：中-重度DAR儿童的肠道微生物多样性与健康儿童相比未见明显变化，但菌群结构比例发生失衡，多种特定微生物的丰度发生显著改变，并伴有部分肠道微生物功能通路的改变。.
Prevotella
Eubacterium
Dysbiosis
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Bifidobacterium longum
Actinomycetaceae
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Dysbiosis
Bifidobacterium breve
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