A simple alternative determination of the formation constant for the inclusion complex between rutin and β-cyclodextrin
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Inclusion compound
Constant (computer programming)
The composition and stability constant of a UO2(II)-rutin complex in 70% ethanol were determined by suitable spectrophotometric methods and pH measurement. UO2(II) ion and rutin (3,3',4',5,7-pentahydroxyflavone-3-rhamnoglucoside) form a 1:1 complex in which the UO2(II) ion is linked to rutin through the carbonyl and 5-hydroxyl group. The concentration stability constant of the complex, log β1, ranged from 6.57 at pH 4.00 to 4.72 at pH 7.00. Conditions for spectrophotometric determination of rutin, by complex formation with UO2(II) ion, were investigated. Beer's law was obeyed in the concentration range from 1.0 × 10-5 to 2.0 × 10-4 M for rutin. Determination of rutin in Rutinion forte tablets was demonstrated. Keywords: Complex; rutin; uranilnitrate; stability constant; spectrophotometric methods
Complex formation
Spectrophotometry
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[Objective] To compare the inclusion effect of different types of cyclodextrin(α- cyclodextrin,β- cyclodextrin,γ- cyclodextrin) on zedoary turmeric oil,study the best preparation technology of zedoary turmeric oil and cyclodextrin inclusion compound.[Methods] Using the saturated water solution method,α-cyclodextrin,β-cyclodextrin,γ-cyclodextrin zedoary turmeric oil inclusion compound were prepared and using X ray diffraction method the inclusion effect was identified;using L9(34) orthogonal experimental design,the best preparation technology of zedoary turmeric oil and cyclodextrin inclusion compound was optimized.[Results] Under the same conditions,α-cyclodextrin and zedoary turmeric oil could not form inclusion compound,the inclusion rate of γ-cyclodextrin and β-cyclodextrin inclusion rate were almost the same.Taking the inclusion rate and oil utilizing rate as the indexes,the optimum process of zedoary turmeric oil and β-cyclodextrin inclusion compound was 8∶1 of A3B1C2: β-cyclodextrin: Oil(g/mL),the inclusion temperature was 40 ℃ and the inclusion time was 90 min.According to the best prescription process parameters 3 experiments were repeatedly performed.It was found that the volatile oil inclusion rate was 90.90% and the oil utilizing rate was 66.67%,RSD2.7%.The results showed that physical mixture of X ray spectrum was different between inclusion complexes with cyclodextrins and volatile oil with cyclodextrin.[Conclusion] The technology is reasonable and the cost can be saved when selecting β-cyclodextrin inclusion zedoary turmeric oil.
Inclusion compound
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Aim To establish a method to measure the inclusion constant of supramolecular compound by capillary electrophoresis. In addition, by employing this method to investigate the intensity of interaction of antibacterial sodium houttuyfonate and β-cyclodextrin, and to measure the stability constant of the inclusion compound formed. Methods The mobilities of houttuyfonate were measured in solutions with different concentrations of β-cyclodextrin by capillary electrophoresis. The stability constant of the inclusion compound was calculated by peak-shift model mathematically. Results The mobility of houttuyfonate shifts from its original value with the increased concentration of β-cyclodextrin. When the limit was reached, the mobility of sodium houttuyfonate did not change even by increasing the concentration of β-cyclodextrin. The reproducibility is well, and the logarithm of inclusion constant obtained was 3.81 regardless of β- cyclodextrin concentration added. The interaction of the sodium houttuyfonate and β-cyclodextrin is strong. If they blended at a radio of 1∶1 , the transform rate of sodium houttuyfonate is 98%. The value of stability constant of supramolecular inclusion compound is independent of concentration of β-cyclodextrin. Conclusion This technique of capillary electrophoresis can be used to the measurement of inclusion constant of drug with β-cyclodextrin. Furthermore, it can be applied to research the stability of inclusion compound drugs in physiological condition.
Inclusion compound
Binding constant
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Objective To develop a new method for the preparation of a solid inclusion compound and investigate the effects of temperature,time,crystallinity of β-cyclodextrin on inclusion compound formation by the heating method.Method A mixture of benzoic acid(BA) and β-cyclodextrin was sealed in a container,and heated at the desired temperature for the specified time.Results The results indicate that BA molecules were included in the β-cyclodextrin cavity by sealed heating.The results also revealed that mixing ratio,temperature,time,crystallinity of β-cyclodextrin are important parameters which affect the formation of inclusion compound by the sealed heating method.Conclusions BA is included in β-cyclodextrin molecules by heating at (90 ℃) for (3 h) in ampoules,and amorphous β-cyclodextrin system shows higher combing ratio than the crystalline β-cyclodextrin system.
Benzoic acid
Inclusion compound
Ampoule
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In order to improve the solubility of Rutin in aqueous solution and the stability, the inclusion compound of Rutin with beta-CD was manufacted by saturated aqueous solution method. The formation of the inclusion compound was confirmed by IR spectroscopy and differential scauning calorimetry. The content of Rutin in inclusion compound was determined by UV. The results indicated that the inclusion compound of Rutin with beta-CD was formed. Quantitative analysis demmonstrated that the molecular ratio of Rutin to beta-CD in the complex was 1:1 with the inclusion constant of 283.79 L/mol at 30 degrees C. The dissolubility of Rutin in water has added to 643.19 mg/L (20 degrees C).
Inclusion compound
Beta-Cyclodextrins
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The technological process of β-cyclodextrin inclusion compound of volatile oil in Xiangsha Yangwei Preparation was studied by orthogonal experiment design.The optimum preparation condition was a proportion of 1∶8(ml:g) for oil to β-cyclodextrin,stirring for 1 hour at 55℃.Then the characters of volatile oil before and after being included were analyzed by GC-MS.It is indicated that β_cyclodextrin inclusion compound can more effectively protect the active components of volatile oil.
Inclusion compound
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Inclusion compound
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The effect of cyclodextrins on the chemical stability of some mitomycins in acidic medium has been investigated. A stability indicating UV spectrophotometry method has been used to determine overall degrad ation rate constants and Lineweaver-Burk plots were used to calculate the stability constants of the mitomycin-cyclodextrin complexes as well as the rate constants for degradation of the mitomycin guest molecules in the various inclusion complexes. The influences of various parameters such as the structures of the cyclodextrins and mitomycins, the cyclo dextrin concentration and the pH are investigated. It appears that in acidic media (pH region 1-5) mitomycin-cyclodextrin complexation occurs. Mitomycin-y-cyclodextrin complexation is most favourable. All mitomycin y-cyclodextrin complexes studied degrade with lower velocities compared to the free drugs. The degradation of the mitomycin guest molecules seems to decrease with higher values of the stability constants of the mitomycin-cyclodextrin inclusion complexes. However, this straight for ward relationship may be complicated by changes in the inclusion mode on changing the chemical structures of the mitomycin guest compounds.
Mitomycin C
Inclusion compound
Degradation
Chemical Stability
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