Technique and results of ovarian microsurgery in preparation for in vitro fertilization
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Surgical method of embryo collection and embryo transfer was used in rabbit. PMSG was used for superovulation. Out of the 41 embryos collected from 6 donors, 30 embryos were transferred in 7 recipients. Out of 7 embryo transferred recipients, 2 kidded giving birth to 7 kids.
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Embryo transfer of pre-implantation embryos to surrogate dams is a key technique for the hygienic sanitation of strains, cryopreservation, in vitro fertilization, genetic modification and engineering. However, the effects of several parameters, such as the number of transferred embryos, on the success of embryo transfer are not well studied. In this retrospective study, we reanalysed 1320 embryo transfers of two-cell embryos originating from genetically altered donors, which were performed under routine conditions in our facility over a period of 5 years. Of them, 453 embryo transfers were done with freshly collected embryos and 867 transfers were performed with cryopreserved embryos. Despite the fact that the genetic background of the embryo donors was quite heterogeneous, we found that the transfer of ≥ 21 embryos reduced the success of embryo transfers for freshly collected embryos in correlation with the number of pregnancies and born pups, whereas this was not the case for transfer in the cryopreservation group. Most pregnancies were achieved after embryo transfer of 10–20 freshly collected embryos (90.4%), which dropped to 37.5% if more embryos were transferred. The highest pregnancy rates in the cryopreservation group were achieved if 15–17 embryos were transferred (62.9%). Despite the fact that the precise substrains were only rarely defined, we confirmed that beside the number of transferred embryos, the genetic background of the donors had an influence on the success of embryo transfer. Significantly more embryos in a C57BL/6 background developed to term than embryos on a BALB/c background.
Embryo cryopreservation
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Summary. Two groups of men were retrospectively selected according to their observed success in in-vitro fertilization. Seminal and post-migration sperm samples from a low fertilization rate group (≤33% cleaved embryos) have been compared to results obtained from a high fertilization rate group (≥ 66%). It was found that a low mean value of the amplitude of lateral sperm head displacement and an increased percentage of abnormal acrosomes were related to in-vitro fertilization failure. None of the individual sperm factors studied was found to determine in-vitro fertilization success with certainty; only when they were considered in combination was it possible to predict the likelihood of successful in-vitro fertilization of human oocytes.
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AIM: To explore the relations of the strains of embryo and pseudopregnant recipient to live birth rate of embryo transfer and the feasibility of mixed transfer of different embryo strains. METHODS: A total of 362 embryos collected from 24 B6CF1 mice (F1 generation of Balb/c male and C578BL/6 emale mice) and 14 kunming (KM) mice were transferred into 24 mice of the 2 strains (8 B6CF1 mice and 16 KM mice)respectively for comparing the live birth rate. RESULTS: The birth rate of B6CF1 embryos transferred into the pseudopregnant mice of same strain were much higher (32.0%) than that of KM mice homogenous embryos (4.0%); the birth rate of mixed transferring of the 2 embryo strains were 25.0% of B6CF1 and 15.0% of KM mice. Except for homogenous embryo transfer, cross-strain embryo transfer was also performed in B6CF1 and KM mice. Among them, the total birth rate in embryo-transferred B6CF1 pseudopregnant mice (29.0%) was obviously higher than that in KM pseudopregnant mice (7.0%). As for embryo strain, the birth rate of B6CF1 strain embryo transferring (16.0%) was higher than that of KM embryo transferring (11.0%). CONCLUSION: The live birth rate of embryo transfer could be elevated by selecting mouse strain cautiously. Both cross-strain and mixed-strain embryo transfer are feasible, but homogenous embryo transfer is a priority. B6CF1 mouse is a better embryo transfer candidate than KM mouse.
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Mouse strain
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Objective To evaluate the influence of psychological intervention on pregnancy rate of in vitro fertilization-embryo transfer.Methods Using the key words,including in vitro fertilization-embryo transfer and psychological,the data were retrieved from CNKI,CBM,VIP,Wanfang and PubMed.The quality of eligible studies was evaluated by two reviews independently and Meta-analysis was conducted on studies.Results Totally thirteen Chinese studies and three foreign studies were included.Conclusions Psychological intervention using in the process of in vitro fertilization-embryo transfer can improve the pregnancy rate,which is worthy of clinical application.
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Psychological nursing; Fertilization in vitro; Embryo transfer, Meta analysis
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Embryo transfer is a crucial step in IVF-embryo transfer cycles. Several studies have explored transmyometrial embryo transfer, but although this procedure has several favourable characteristics, its role in assisted reproduction has not yet been established. Junctional zone (JZ) contractions during embryo transfer are associated with a negative outcome and factors which increase JZ contractions should be avoided.In this study, we have investigated the effect of transmyometrial embryo transfer on JZ contractions. Ten patients with a previously difficult embryo transfer, or a difficult mock embryo transfer, underwent transmyometrial embryo transfer. Before and after this procedure a transvaginal ultrasound scan was performed and this was recorded on videotape for 5 min. The recordings were digitized and then analysed for JZ contractions.Transmyometrial embryo transfer causes a significant increase in JZ contractions.The increase in JZ contractions after transmyometrial embryo transfer forms a theoretical objection to this procedure. However, its alternative, a difficult transcervical embryo transfer, is also associated with an increase in JZ contractions. We therefore suggest a large prospective study to investigate the most effective method of embryo transfer in cases where a difficult transcervical embryo transfer is anticipated due to cervical factors.
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Does ultrasound guided embryo transfer improve pregnancy rate in in vitro fertilization? Does ultrasound guided embryo transfer improve pregnancy rate in in vitro fertilization? Does ultrasound guided embryo transfer improve pregnancy rate in in vitro fertilization?
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